Thymidine Incorporation by the Microbial Community of Standing Dead Spartina alterniflora.

Abstract:

:Thymidine incorporation by the microbial community on standing dead leaves of Spartina alterniflora did not obey many of the assumptions inherent in the use of the technique in planktonic systems. Incorporation rates of [methly-H]thymidine were nonsaturable over a wide concentration range (10 to 10 nM). Owing to metabolism by both fungi and bacteria, a major fraction of the radiolabel (mean, 48%) appeared in protein. Extraction of the radiolabeled macromolecules was inefficient, averaging 8.8%. Based on an empirically derived conversion factor, 4 x 10 cells . mol of thymidine, doubling times ranged from 4 to 69 h for the epiphytic bacterial assemblage.

journal_name

Appl Environ Microbiol

authors

Fallon RD,Newell SY

doi

10.1128/AEM.52.5.1206-1208.1986

keywords:

subject

Has Abstract

pub_date

1986-11-01 00:00:00

pages

1206-8

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

52

pub_type

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