Terminal olefin (1-alkene) biosynthesis by a novel p450 fatty acid decarboxylase from Jeotgalicoccus species.

Abstract:

:Terminal olefins (1-alkenes) are natural products that have important industrial applications as both fuels and chemicals. However, their biosynthesis has been largely unexplored. We describe a group of bacteria, Jeotgalicoccus spp., which synthesize terminal olefins, in particular 18-methyl-1-nonadecene and 17-methyl-1-nonadecene. These olefins are derived from intermediates of fatty acid biosynthesis, and the key enzyme in Jeotgalicoccus sp. ATCC 8456 is a terminal olefin-forming fatty acid decarboxylase. This enzyme, Jeotgalicoccus sp. OleT (OleT(JE)), was identified by purification from cell lysates, and its encoding gene was identified from a draft genome sequence of Jeotgalicoccus sp. ATCC 8456 using reverse genetics. Heterologous expression of the identified gene conferred olefin biosynthesis to Escherichia coli. OleT(JE) is a P450 from the cyp152 family, which includes bacterial fatty acid hydroxylases. Some cyp152 P450 enzymes have the ability to decarboxylate and to hydroxylate fatty acids (in α- and/or β-position), suggesting a common reaction intermediate in their catalytic mechanism and specific structural determinants that favor one reaction over the other. The discovery of these terminal olefin-forming P450 enzymes represents a third biosynthetic pathway (in addition to alkane and long-chain olefin biosynthesis) to convert fatty acid intermediates into hydrocarbons. Olefin-forming fatty acid decarboxylation is a novel reaction that can now be added to the catalytic repertoire of the versatile cytochrome P450 enzyme family.

journal_name

Appl Environ Microbiol

authors

Rude MA,Baron TS,Brubaker S,Alibhai M,Del Cardayre SB,Schirmer A

doi

10.1128/AEM.02580-10

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

1718-27

issue

5

eissn

0099-2240

issn

1098-5336

pii

AEM.02580-10

journal_volume

77

pub_type

杂志文章
  • Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains.

    abstract::Listeria monocytogenes is a food pathogen that can attach on most of the surfaces encountered in the food industry. Biofilms are three-dimensional microbial structures that facilitate the persistence of pathogens on surfaces, their resistance toward antimicrobials, and the final contamination of processed goods. So fa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00255-08

    authors: Rieu A,Briandet R,Habimana O,Garmyn D,Guzzo J,Piveteau P

    更新日期:2008-07-01 00:00:00

  • Ammonium production by dissimilatory nitrate reducers isolated from baltic sea water, as indicated by N study.

    abstract::Bacteria able to perform dissimilatory nitrate reduction to ammonium were isolated from low-oxygen masses in the Baltic Sea. In liquid media enriched with NO(3) and incubated anaerobically, the NH(4)-producing isolates transformed 25 to 72% of the NO(3) to NH(4). ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.5.1241-1243.1982

    authors: Samuelsson MO,Rönner U

    更新日期:1982-11-01 00:00:00

  • Cold shock induction of thermal sensitivity in Listeria monocytogenes.

    abstract::Cold shock at 0 to 15 degrees C for 1 to 3 h increased the thermal sensitivity of Listeria monocytogenes. In a model broth system, thermal death time at 60 degrees C was reduced by up to 45% after L. monocytogenes Scott A was cold shocked for 3 h. The duration of the cold shock affected thermal tolerance more than did...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4345-4350.2000

    authors: Miller AJ,Bayles DO,Eblen BS

    更新日期:2000-10-01 00:00:00

  • Comparison of single- and multilocus genetic diversity in the protozoan parasites Cryptosporidium parvum and C. hominis.

    abstract::The genotyping of numerous isolates of Cryptosporidium parasites has led to the definition of new species and a better understanding of the epidemiology of cryptosporidiosis. A single-locus genotyping method based on the partial sequence of a polymorphic sporozoite surface glycoprotein gene (GP60) has been favored by ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01268-10

    authors: Widmer G,Lee Y

    更新日期:2010-10-01 00:00:00

  • Optimum pH levels for eluting enteroviruses from sludge solids with beef extract.

    abstract::This study demonstrates that elution of enteroviruses from a mixture of primary- and activated-sludge solids with beef extract at pH 9.2 +/- 0.2 may be less efficient than elution with beef extract at pH 7.2 +/- 0.2 and that elution of enteroviruses from extended-aeration-sludge solids with beef extract is at best no ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.7.1880-1881.1988

    authors: Berg G,Sullivan G

    更新日期:1988-07-01 00:00:00

  • Effect of benzoic Acid on growth yield of yeasts differing in their resistance to preservatives.

    abstract::Yeasts grown in the presence of benzoic acid tolerated 40 to 100% higher benzoic acid concentrations than did those grown in the absence of weak-acid-type preservatives. They also accumulated less benzoate in the presence of glucose. In chemostat cultures, benzoic acid reduced growth yield and the rate of cell product...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.8.2091-2095.1988

    authors: Warth AD

    更新日期:1988-08-01 00:00:00

  • Characterization of a granulovirus isolated from Epinotia aporema Wals. (Lepidoptera: Tortricidae) larvae.

    abstract::A granulovirus (GV) isolated from Epinotia aporema (Lepidoptera: Tortricidae)-a major soybean pest-was studied in terms of its main morphological, biochemical, and biological properties. The ovoidal occlusion bodies were 466 by 296 nm in size, and their most prominent protein had an apparent molecular mass of 29 kDa. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3702-3706.2001

    authors: Sciocco-Cap A,Parola AD,Goldberg AV,Ghiringhelli PD,Romanowski V

    更新日期:2001-08-01 00:00:00

  • Purification, characterization, and heterologous expression in Fusarium venenatum of a novel serine carboxypeptidase from Aspergillus oryzae.

    abstract::A novel serine carboxypeptidase (EC 3.4.16.1) was found in an Aspergillus oryzae fermentation broth and was purified to homogeneity. This enzyme has a molecular weight of ca. 67,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its specific activity is 21 U/mg for carbobenzoxy (Z)-Al...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.8.3298-3303.1999

    authors: Blinkovsky AM,Byun T,Brown KM,Golightly EJ

    更新日期:1999-08-01 00:00:00

  • shift from acetoclastic to H2-dependent methanogenesis in a west Siberian peat bog at low pH values and isolation of an acidophilic Methanobacterium strain.

    abstract::Methane production and archaeal community composition were studied in samples from an acidic peat bog incubated at different temperatures and pH values. H(2)-dependent methanogenesis increased strongly at the lowest pH, 3.8, and Methanobacteriaceae became important except for Methanomicrobiaceae and Methanosarcinaceae...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02413-06

    authors: Kotsyurbenko OR,Friedrich MW,Simankova MV,Nozhevnikova AN,Golyshin PN,Timmis KN,Conrad R

    更新日期:2007-04-01 00:00:00

  • Microbial community analysis of field-grown soybeans with different nodulation phenotypes.

    abstract::Microorganisms associated with the stems and roots of nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans [Glycine max (L.) Merril] were analyzed by ribosomal intergenic transcribed spacer analysis (RISA) and automated RISA (ARISA). RISA of stem samples detected no bands specific...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00833-08

    authors: Ikeda S,Rallos LE,Okubo T,Eda S,Inaba S,Mitsui H,Minamisawa K

    更新日期:2008-09-01 00:00:00

  • Ecological constraints limit the fitness of fungal hybrids in the Heterobasidion annosum species complex.

    abstract::The ability of two closely related species to maintain species boundaries in spite of retained interfertility between them is a documented driving force of speciation. Experimental evidence to support possible interspecific postzygotic isolation mechanisms for organisms belonging to the kingdom Fungi is still missing....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01135-07

    authors: Garbelotto M,Gonthier P,Nicolotti G

    更新日期:2007-10-01 00:00:00

  • Transformation of Escherichia coli with DNA from Saccharomyces cerevisiae cell lysates.

    abstract::We developed a system to monitor the transfer of heterologous DNA from a genetically manipulated strain of Saccharomyces cerevisiae to Escherichia coli. This system is based on a yeast strain that carries multiple integrated copies of a pUC-derived plasmid. The bacterial sequences are maintained in the yeast genome by...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5303-5306.1999

    authors: Adam AC,González-Blasco G,Rubio-Texeira M,Polaina J

    更新日期:1999-12-01 00:00:00

  • Characteristics and Adaptability of Some New Isolates of Clostridium thermocellum.

    abstract::Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.3.475-477.1985

    authors: Bender J,Vatcharapijarn Y,Jeffries TW

    更新日期:1985-03-01 00:00:00

  • Purification and Characterization of Three Chitosanase Activities from Bacillus megaterium P1.

    abstract::Bacillus megaterium P1, a bacterial strain capable of hydrolyzing chitosan, was isolated from soil samples. Chitosan-degrading activity was induced by chitosan but not by its constituent d-glucosamine. Extracellular secretion of chitosanase reached levels corresponding to 1 U/ml under optimal conditions. Three chitosa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.844-848.1990

    authors: Pelletier A,Sygusch J

    更新日期:1990-04-01 00:00:00

  • Viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in Finland.

    abstract::During an outbreak of paralytic poliomyelitis in Finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater Helsinki district and in 13 other cities o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.2.371-374.1988

    authors: Pöyry T,Stenvik M,Hovi T

    更新日期:1988-02-01 00:00:00

  • Response of Archaeal communities in beach sediments to spilled oil and bioremediation.

    abstract::While the contribution of Bacteria to bioremediation of oil-contaminated shorelines is well established, the response of Archaea to spilled oil and bioremediation treatments is unknown. The relationship between archaeal community structure and oil spill bioremediation was examined in laboratory microcosms and in a bio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.5.2614-2620.2004

    authors: Röling WF,de Brito Couto IR,Swannell RP,Head IM

    更新日期:2004-05-01 00:00:00

  • Discrimination of psychrotrophic and mesophilic strains of the Bacillus cereus group by PCR targeting of major cold shock protein genes.

    abstract::Detection of psychrotrophic strains (those able to grow at or below 7 degreesC) of the Bacillus cereus group (Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides) in food products is at present extremely slow with conventional microbiology. This is due to an inability to discriminate these cold-adapted stra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.9.3525-3529.1998

    authors: Francis KP,Mayr R,von Stetten F,Stewart GS,Scherer S

    更新日期:1998-09-01 00:00:00

  • A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture.

    abstract::A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1383-1390.2003

    authors: Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

    更新日期:2003-03-01 00:00:00

  • Sporicidal activity of synthetic antifungal undecapeptides and control of Penicillium rot of apples.

    abstract::The antifungal activity of cecropin A(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. A set of 15 sequences was screened in vitro against Fusarium oxysporum, Penicillium expansum, Aspergillus niger, and Rhizopus stolonifer. Most compounds were hig...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00711-09

    authors: Badosa E,Ferré R,Francés J,Bardají E,Feliu L,Planas M,Montesinos E

    更新日期:2009-09-01 00:00:00

  • Anti-Candida albicans activity of Pichia anomala as determined by a growth rate reduction assay.

    abstract::Killer toxin activity of Pichia anomala WC65 appeared fungicidal for P. bimundalis WC38 and fungistatic for Candida albicans RC1. Inhibitory activity against sensitive C. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. The plot of toxin concentrations aga...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.5.1099-1103.1988

    authors: Sawant AD,Abdelal AT,Ahearn DG

    更新日期:1988-05-01 00:00:00

  • Role of chemotaxis in the ecology of denitrifiers.

    abstract::A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO(3) and NO(2) by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.1.109-114.1985

    authors: Kennedy MJ,Lawless JG

    更新日期:1985-01-01 00:00:00

  • Reduction of Escherichia coli O157:H7 populations in cattle by addition of colicin E7-producing E. coli to feed.

    abstract::A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6053-6060.2004

    authors: Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

    更新日期:2004-10-01 00:00:00

  • Raman spectroscopy-compatible inactivation method for pathogenic endospores.

    abstract::Micro-Raman spectroscopy is a fast and sensitive tool for the detection, classification, and identification of biological organisms. The vibrational spectrum inherently serves as a fingerprint of the biochemical composition of each bacterium and thus makes identification at the species level, or even the subspecies le...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02481-09

    authors: Stöckel S,Schumacher W,Meisel S,Elschner M,Rösch P,Popp J

    更新日期:2010-05-01 00:00:00

  • Volatile compounds originating from mixed microbial cultures on building materials under various humidity conditions.

    abstract::We examined growth of mixed microbial cultures (13 fungal species and one actinomycete species) and production of volatile compounds (VOCs) in typical building materials in outside walls, separating walls, and bathroom floors at various relative humidities (RHs) of air. Air samples from incubation chambers were adsorb...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.8.2914-2919.1998

    authors: Korpi A,Pasanen AL,Pasanen P

    更新日期:1998-08-01 00:00:00

  • Styrofoam cup-membrane assembly for studying microorganism-root interactions.

    abstract::An assembly consisting of Styrofoam cups with membranes of varying porosities was developed to study microorganism-root interactions. The assembly permitted uniform distribution of a bacterium in soil and was simple, easy to use, and disposable. In tests with the bacterium Pseudomonas solanacearum, little difference i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.5.1291-1294.1989

    authors: Hartel PG,Billingsley JW,Williamson JW

    更新日期:1989-05-01 00:00:00

  • Susceptibility of suspended and surface-attached Salmonella enteritidis to biocides and elevated temperatures.

    abstract::The differential resistance of substratum-attached, detached, and planktonic cells of Salmonella enteritidis phage type 4 was studied by using several inimical processes and in vivo bioluminescence as a nondestructive, real-time reporter of metabolic activity. Bioluminescence in this strain was mediated by a construct...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.5.1731-1738.1995

    authors: Dhir VK,Dodd CE

    更新日期:1995-05-01 00:00:00

  • Cometabolic degradation of dibenzofuran by biphenyl-cultivated Ralstonia sp. strain SBUG 290.

    abstract::Cells of the gram-negative bacterium Ralstonia sp. strain SBUG 290 grown in the presence of biphenyl are able to cooxidize dibenzofuran which has been 1,2-hydroxylated. Meta cleavage of the 1, 2-dihydroxydibenzofuran between carbon atoms 1 and 9b produced 2-hydroxy-4-(3'-oxo-3'H-benzofuran-2'-yliden)but-2-enoic acid, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4528-4531.2000

    authors: Becher D,Specht M,Hammer E,Francke W,Schauer F

    更新日期:2000-10-01 00:00:00

  • Terminal Olefin Profiles and Phylogenetic Analyses of Olefin Synthases of Diverse Cyanobacterial Species.

    abstract::Cyanobacteria can synthesize alkanes and alkenes, which are considered to be infrastructure-compatible biofuels. In terms of physiological function, cyanobacterial hydrocarbons are thought to be essential for membrane flexibility for cell division, size, and growth. The genetic basis for the biosynthesis of terminal o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00425-18

    authors: Zhu T,Scalvenzi T,Sassoon N,Lu X,Gugger M

    更新日期:2018-06-18 00:00:00

  • Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp.

    abstract::The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.9.2806-2814.1992

    authors: Schendel FJ,Flickinger MC

    更新日期:1992-09-01 00:00:00

  • Effects of High Pressure on Inactivation Kinetics and Events Related to Proton Efflux in Lactobacillus plantarum.

    abstract::Knowledge of the mechanism of pressure-induced inactivation of microorganisms could be helpful in defining an effective, relatively mild pressure treatment as a means of decontamination, especially in combination with other physical treatments or antimicrobial agents. We have studied the effect of high pressure on Lac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.2.509-514.1998

    authors: Wouters PC,Glaasker E,Smelt JP

    更新日期:1998-02-01 00:00:00