Ecological constraints limit the fitness of fungal hybrids in the Heterobasidion annosum species complex.

abstract：:A colorimetric assay for NADPH-dependent, mercuric ion-specific oxidoreductase activity was developed to facilitate the investigation of mercuric reductase gene expression in polluted aquatic ecosystems. Protein molecules extracted directly from unseeded freshwater and samples seeded with Pseudomonas aeruginosa PU21 (...

journal_title：Applied and environmental microbiology

authors： Ogunseitan OA

更新日期：1998-02-01 00:00:00

abstract：:Simultaneous production of penicillic acid and patulin by an atypical strain of Penicillium roqueforti isolated from cheddar cheese is reported. Mycotoxin production was confirmed by thin-layer and gas-liquid chromatography and by ultraviolet, infrared, and mass spectral analyses. Culture extracts were toxic to Bacill...

journal_title：Applied and environmental microbiology

authors： Olivigni FJ,Bullerman LB

更新日期：1978-02-01 00:00:00

abstract：:Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypotheti...

journal_title：Applied and environmental microbiology

authors： Simmons K,Rempel H,Block G,Forgetta V,Vaillancourt R Jr,Malouin F,Topp E,Delaquis P,Diarra MS

更新日期：2014-03-01 00:00:00

abstract：:Rhizobiophage V, isolated from soil in the vicinity of soybean roots, was strongly lytic on Bradyrhizobium japonicum 123B (USDA 123) but only mildly lytic on strain L4-4, a chemically induced small-colony mutant of 123. Numerous bacteriophage-resistant variants were isolated from L4-4 infected with phage V; two were s...

journal_title：Applied and environmental microbiology

authors： Abebe HM,Sadowsky MJ,Kinkle BK,Schmidt EL

更新日期：1992-10-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00

abstract：:Similar populations of hypochlorite-treated spores were enumerated from two crops of Clostridium botulinum 12885A produced by the same procedure; however, germination required different L-alanine concentrations. Lactate permitted the germination of spores from both crops with suboptimal L-alanine concentrations. The d...

journal_title：Applied and environmental microbiology

authors： Foegeding PM,Busta FF

更新日期：1983-04-01 00:00:00

abstract：:The influence of modified-atmosphere packaging, storage temperature, and time on survival and growth of Escherichia coli O157:H7 inoculated onto shredded lettuce, sliced cucumber, and shredded carrot was determined. Growth of psychotrophic and mesophilic microorganisms and changes in pH and sensory qualities of vegeta...

journal_title：Applied and environmental microbiology

authors： Abdul-Raouf UM,Beuchat LR,Ammar MS

更新日期：1993-07-01 00:00:00

abstract：:The importance of viruses in marine microbial ecology has been established over the past decade. Specifically, viruses influence bacterial abundance and community composition through lysis and alter bacterial genetic diversity through transduction and lysogenic conversion. By contrast, the abundance and distribution o...

journal_title：Applied and environmental microbiology

authors： Williamson KE,Radosevich M,Wommack KE

更新日期：2005-06-01 00:00:00

abstract：:The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oysters and Greenshell mussels ...

journal_title：Applied and environmental microbiology

authors： Cruz CD,Hedderley D,Fletcher GC

更新日期：2015-04-01 00:00:00

abstract：:A Bacillus sp. capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from Cape Canerval soil samples. The organism was isolated on a glucose-mineral salts medium with phosphate deleted. Anaerobic cultivation of this isolate resulted in decreases in the hypophosphite or phosphite conc...

journal_title：Applied and environmental microbiology

authors： Foster TL,Winans L Jr,Helms SJ

更新日期：1978-05-01 00:00:00

abstract：:Variations in the thymidine incorporation rate, bacterial abundance, and mean cell volumes in the surface water (0.5 m) of the Baltic Sea in spring and summer were compared in studies with different spatial scales (570 nautical miles [nmi] [ca. 1056 km], 220 nmi [ca. 407 km], 24 nmi [ca. 44 km], 12 nmi [ca. 22 km], an...

journal_title：Applied and environmental microbiology

authors： Heinänen A,Kuparinen J

更新日期：1991-11-01 00:00:00

abstract：:Strain UHM-5, a pSym Exo derivative of the aluminum-tolerant Rhizobium leguminosarum bv. phaseoli strain CIAT899, was equally tolerant of aluminum (Al) as the parental culture. Dialyzed culture supernatants of the wild-type cells grown in YEM broth (10 cells ml) contained 185 mug of glucose equivalents ml whereas UHM-...

journal_title：Applied and environmental microbiology

authors： Kingsley MT,Bohlool BB

更新日期：1992-04-01 00:00:00

abstract：:Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >...

journal_title：Applied and environmental microbiology

authors： Stach JE,Maldonado LA,Masson DG,Ward AC,Goodfellow M,Bull AT

更新日期：2003-10-01 00:00:00

abstract：:Over 1,400 water samples were collected biweekly over 6 years from an intermittent stream protected and unprotected from pasturing cattle. The samples were monitored for host-specific Bacteroidales markers, Cryptosporidium species/genotypes, viruses and coliphages associated with humans or animals, and bacterial zoono...

journal_title：Applied and environmental microbiology

authors： Wilkes G,Brassard J,Edge TA,Gannon V,Jokinen CC,Jones TH,Marti R,Neumann NF,Ruecker NJ,Sunohara M,Topp E,Lapen DR

更新日期：2013-10-01 00:00:00

abstract：:We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mappin...

journal_title：Applied and environmental microbiology

authors： Katzen F,Becker A,Ielmini MV,Oddo CG,Ielpi L

更新日期：1999-01-01 00:00:00

abstract：:The impact of host nutrition on symbiont regulation in the pea aphid Acyrthosiphon pisum was investigated. The population density of the obligate symbiont Buchnera aphidicola positively correlated with dietary nitrogen levels. In contrast, the population density of the facultative symbiont Serratia symbiotica increase...

journal_title：Applied and environmental microbiology

authors： Wilkinson TL,Koga R,Fukatsu T

更新日期：2007-02-01 00:00:00

abstract：:A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was al...

journal_title：Applied and environmental microbiology

authors： Heylen K,Ettwig K,Hu Z,Jetten M,Kartal B

更新日期：2012-04-01 00:00:00

abstract：:Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms in...

journal_title：Applied and environmental microbiology

authors： Wang IK,Lai HC,Yu CJ,Liang CC,Chang CT,Kuo HL,Yang YF,Lin CC,Lin HH,Liu YL,Chang YC,Wu YY,Chen CH,Li CY,Chuang FR,Huang CC,Lin CH,Lin HC

更新日期：2012-02-01 00:00:00

abstract：:We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty of the tool described here is the inclusion of a minimal bacteri...

journal_title：Applied and environmental microbiology

authors： Petersen KV,Martinussen J,Jensen PR,Solem C

更新日期：2013-06-01 00:00:00

abstract：:Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features (i) one short targ...

journal_title：Applied and environmental microbiology

authors： Maruyama F,Kenzaka T,Yamaguchi N,Tani K,Nasu M

更新日期：2005-12-01 00:00:00

abstract：:α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...

journal_title：Applied and environmental microbiology

authors： Nikitkova AE,Haase EM,Scannapieco FA

更新日期：2013-01-01 00:00:00

abstract：:This study investigated the abundance, distribution, and composition of microbial communities at the watershed scale in a boreal peatland within the Marcell Experimental Forest (MEF), Minnesota, USA. Through a close coupling of next-generation sequencing, biogeochemistry, and advanced analytical chemistry, a biogeoche...

journal_title：Applied and environmental microbiology

authors： Lin X,Tfaily MM,Steinweg JM,Chanton P,Esson K,Yang ZK,Chanton JP,Cooper W,Schadt CW,Kostka JE

更新日期：2014-06-01 00:00:00

abstract：:A recombinant Escherichia coli strain carrying a plasmid with an antibiotic resistance marker and expressing the green fluorescent protein was inoculated at a concentration of 3.8 x 10(8) CFU/g into direct-cut wheat (348 g of dry matter kg(-1)), wilted wheat (450 g of dry matter kg(-1)), and corn (375 g of dry matter ...

journal_title：Applied and environmental microbiology

authors： Chen Y,Sela S,Gamburg M,Pinto R,Weinberg ZG

更新日期：2005-09-01 00:00:00

abstract：:Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are...

journal_title：Applied and environmental microbiology

authors： McCabe PM,Van Alfen NK

更新日期：1999-12-01 00:00:00

abstract：:beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...

journal_title：Applied and environmental microbiology

authors： Araujo A,Ward OP

更新日期：1990-06-01 00:00:00

abstract：:Graded doses of pure ochratoxin A (0, 0.5, 1.0, 2.0, 4.0, and 8.0 microgram of toxin per g of feed) were incorporated into a commercial diet which was fed to chickens from hatching to 3 weeks of age, at which time the experiments were terminated. Liver glycogen levels were elevated significantly (P less than 0.05) by ...

journal_title：Applied and environmental microbiology

authors： Huff WE,Doerr JA,Hamilton PB

更新日期：1979-01-01 00:00:00

abstract：:Most of the edible mushrooms cannot be cultivated or have low yield under industrial conditions, partially due to the lack of knowledge on how basidioma (fruiting body) development is regulated. From winter mushroom (Flammulina velutipes), one of the most popular industrially cultivated mushrooms, a transcription fact...

journal_title：Applied and environmental microbiology

authors： Wu T,Hu C,Xie B,Zhang L,Yan S,Wang W,Tao Y,Li S

更新日期：2019-11-27 00:00:00

abstract：:1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli express...

journal_title：Applied and environmental microbiology

authors： Altaras NE,Cameron DC

更新日期：1999-03-01 00:00:00

abstract：:Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitat...

journal_title：Applied and environmental microbiology

authors： Hammes F,Boon N,Vital M,Ross P,Magic-Knezev A,Dignum M

更新日期：2011-02-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae DPM1 gene (coding for dolichylphosphate mannose synthase) in Trichoderma reesei (Hypocrea jecorina) increases the intensity of protein glycosylation and secretion and causes ultrastructural changes in the fungal cell wall. In the present work, we undertook further biochemical...

journal_title：Applied and environmental microbiology

authors： Perlińska-Lenart U,Orlowski J,Laudy AE,Zdebska E,Palamarczyk G,Kruszewska JS

更新日期：2006-12-01 00:00:00