Abstract:
:Strain UHM-5, a pSym Exo derivative of the aluminum-tolerant Rhizobium leguminosarum bv. phaseoli strain CIAT899, was equally tolerant of aluminum (Al) as the parental culture. Dialyzed culture supernatants of the wild-type cells grown in YEM broth (10 cells ml) contained 185 mug of glucose equivalents ml whereas UHM-5 culture supernatants yielded 2 mug of glucose ml. The Exo derivative and the parental strain gave essentially similar growth in medium containing from 0 to 300 muM Al, indicating that the pSym of CIAT899, and extracellular polysaccharide, were not involved in the aluminum tolerance of this strain. However, increasing the level of Al from 80 to 150 muM increased the lag phase, induced a slight killing of the inoculum, and depressed the final populations by about fivefold. Doubling the aluminum concentration from 150 to 300 muM presented a severe aluminum stress to CIAT899 and UHM-5: the inoculum level dropped 10-fold, indicating killing of the inoculum, and remained depressed for ca. 4 days before continuing to grow slowly; the final population was decreased 15-fold relative to that of cultures grown in medium containing 80 muM Al. The production by CIAT899 of other extracellular or intracellular aluminum tolerance factors was investigated in culture by using aluminum-sensitive rhizobia as stress indicators. These experiments, conducted at 80 muM Al, demonstrated that CIAT899 produced neither extracellular nor intracellular products that could alleviate toxicity for the Al-sensitive indicator rhizobia.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kingsley MT,Bohlool BBdoi
10.1128/AEM.58.4.1095-1101.1992keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
1095-101issue
4eissn
0099-2240issn
1098-5336journal_volume
58pub_type
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