Biogenic mineral production by a novel arsenic-metabolizing thermophilic bacterium from the Alvord Basin, Oregon.

Abstract:

:The Alvord Basin in southeast Oregon contains a variety of hydrothermal features which have never been microbiologically characterized. A sampling of Murky Pot (61 degrees C; pH 7.1) led to the isolation of a novel arsenic-metabolizing organism (YeAs) which produces an arsenic sulfide mineral known as beta-realgar, a mineral that has not previously been observed as a product of bacterial arsenic metabolism. YeAs was grown on a freshwater medium and utilized a variety of organic substrates, particularly carbohydrates and organic acids. The temperature range for growth was 37 to 75 degrees C (optimum, 55 degrees C), and the pH range for growth was 6.0 to 8.0 (optimum, pH 7.0 to 7.5). No growth was observed when YeAs was grown under aerobic conditions. The doubling time when the organism was grown with yeast extract and As(V) was 0.71 h. Microscopic examination revealed Gram stain-indeterminate, non-spore-forming, nonmotile, rod-shaped cells, with dimensions ranging from 0.1 to 0.2 microm wide by 3 to 10 microm long. Arsenic sulfide mineralization of cell walls and extracellular arsenic sulfide particulate deposition were observed with electron microscopy and elemental analysis. 16S rRNA gene analysis placed YeAs in the family Clostridiaceae and indicated that the organism is most closely related to the Caloramator and Thermobrachium species. The G+C content was 35%. YeAs showed no detectable respiratory arsenate reductase but did display significant detoxification arsenate reductase activity. The phylogenetic, physiological, and morphological characteristics of YeAs demonstrate that it is an anaerobic, moderately thermophilic, arsenic-reducing bacterium. This organism and its associated metabolism could have major implications in the search for innovative methods for arsenic waste management and in the search for novel biogenic mineral signatures.

journal_name

Appl Environ Microbiol

authors

Ledbetter RN,Connon SA,Neal AL,Dohnalkova A,Magnuson TS

doi

10.1128/AEM.00371-07

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

5928-36

issue

18

eissn

0099-2240

issn

1098-5336

pii

AEM.00371-07

journal_volume

73

pub_type

杂志文章
  • Pseudomonas cepacia suppression of sunflower wilt fungus and role of antifungal compounds in controlling the disease.

    abstract::In a field experiment, Pseudomonas cepacia J82rif and J51rif increased sunflower emergence in the presence of the fungus Sclerotinia sclerotiorum. Pyrrolnitrin, aminopyrrolnitrin, and monochloroaminopyrrolnitrin were isolated from J82 and identified by using thin-layer chromatography, high-performance liquid chromatog...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.5.1760-1763.1992

    authors: McLoughlin TJ,Quinn JP,Bettermann A,Bookland R

    更新日期:1992-05-01 00:00:00

  • Two Naturally Occurring Nuclear Polyhedrosis Virus Variants of Neodiprion sertifer Geoffr. (Hymenoptera; Diprionidae).

    abstract::Two isolates of Neodiprion sertifer (Geoffr.) nuclear polyhedrosis virus from Britain and North America were compared using three biochemical techniques. Alkaline protease assays of polyhedra revealed the presence of endogenous enzyme activity in the British isolate but not in the North American isolate. Sodium dodecy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.1.65-69.1982

    authors: Brown DA

    更新日期:1982-01-01 00:00:00

  • Heteropolysaccharide Formation by Arthrobacter viscosus Grown on Xylose and Xylose Oligosaccharides.

    abstract::Arthrobacter viscosus NRRL B-1973 produces its viscous extracellular polysaccharides (EPS) when grown on media containing xylose or enzymatic xylan hydrolysates. Crude EPS formation from xylose averaged 12 g/liter when initial culture pH was adjusted to 8.0 and total nitrogen was limited to 0.03%. Purified EPS from pe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.11.3501-3507.1992

    authors: Novak JS,Tanenbaum SW,Nakas JP

    更新日期:1992-11-01 00:00:00

  • Modeling the lag time of Listeria monocytogenes from viable count enumeration and optical density data.

    abstract::The following two factors significantly influence estimates of the maximum specific growth rate ( micro (max)) and the lag-phase duration (lambda): (i) the technique used to monitor bacterial growth and (ii) the model fitted to estimate parameters. In this study, nine strains of Listeria monocytogenes were monitored s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.12.5816-5825.2002

    authors: Baty F,Flandrois JP,Delignette-Muller ML

    更新日期:2002-12-01 00:00:00

  • Biomineralization of uranium by PhoY phosphatase activity aids cell survival in Caulobacter crescentus.

    abstract::Caulobacter crescentus is known to tolerate high levels of uranium [U(VI)], but its detoxification mechanism is poorly understood. Here we show that C. crescentus is able to facilitate U(VI) biomineralization through the formation of U-Pi precipitates via its native alkaline phosphatase activity. The U-Pi precipitates...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01050-14

    authors: Yung MC,Jiao Y

    更新日期:2014-08-01 00:00:00

  • Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses.

    abstract::Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.4200-4204.2003

    authors: Halpern M,Gancz H,Broza M,Kashi Y

    更新日期:2003-07-01 00:00:00

  • Chromosomal location plays a role in regulation of aflatoxin gene expression in Aspergillus parasiticus.

    abstract::The nor-1 gene in the filamentous fungus Aspergillus parasiticus encodes a ketoreductase involved in aflatoxin biosynthesis. To study environmental influences on nor-1 expression, we generated plasmid pAPGUSNNB containing a nor-1 promoter-beta-glucuronidase (GUS) (encoded by uidA) reporter fusion with niaD (encodes ni...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.1.306-315.2002

    authors: Chiou CH,Miller M,Wilson DL,Trail F,Linz JE

    更新日期:2002-01-01 00:00:00

  • Reduction of Norwalk virus, poliovirus 1, and bacteriophage MS2 by ozone disinfection of water.

    abstract::Norwalk virus and other human caliciviruses (noroviruses) are major agents of gastroenteritis, and water is a major route of their transmission. In an effort to control Norwalk virus in drinking water, Norwalk virus reduction by bench-scale ozone disinfection was determined using quantitative reverse transcription (RT...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.3975-3978.2003

    authors: Shin GA,Sobsey MD

    更新日期:2003-07-01 00:00:00

  • Oxidation of chloroform in an aerobic soil exposed to natural gas.

    abstract::Acclimation of a sandy soil to an air-natural gas mixture stimulated the biological oxidation of chloroform to carbon dioxide. Acetylene and methane inhibited chloroform oxidation. Chloroform oxidation continued up to 31 days in the absence of methane. Chloroform oxidation rates increased at chloroform concentrations ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.1.203-205.1986

    authors: Strand SE,Shippert L

    更新日期:1986-07-01 00:00:00

  • Transcriptome and secretome analyses of Phanerochaete chrysosporium reveal complex patterns of gene expression.

    abstract::The wood decay basidiomycete Phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. A total of 545 genes were flagged on the basis of signific...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00314-09

    authors: Vanden Wymelenberg A,Gaskell J,Mozuch M,Kersten P,Sabat G,Martinez D,Cullen D

    更新日期:2009-06-01 00:00:00

  • Molecular characterization of races and vegetative compatibility groups in Fusarium oxysporum f. sp. vasinfectum.

    abstract::Restriction fragment length polymorphism (RFLP) and vegetative compatibility analyses were undertaken to assess genetic relationships among 52 isolates of Fusarium oxysporum f. sp. vasinfectum of worldwide origin and representing race A, 3, or 4 on cotton plants. Ten distinct vegetative compatibility groups (VCGs) wer...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.11.4039-4046.1994

    authors: Fernandez D,Assigbese K,Dubois MP,Geiger JP

    更新日期:1994-11-01 00:00:00

  • Growth temperature exerts differential physiological and transcriptional responses in laboratory and wine strains of Saccharomyces cerevisiae.

    abstract::Laboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00602-08

    authors: Pizarro FJ,Jewett MC,Nielsen J,Agosin E

    更新日期:2008-10-01 00:00:00

  • Evolutionary relationships of "Candidatus endobugula" bacterial symbionts and their Bugula bryozoan hosts.

    abstract::Ribosomal gene sequences were obtained from bryozoans in the genus Bugula and their bacterial symbionts; analyses of host and symbiont phylogenetic trees did not support a history of strict cospeciation. Symbiont-derived compounds known to defend host larvae from predation were only detected in two out of four symbiot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02798-07

    authors: Lim-Fong GE,Regali LA,Haygood MG

    更新日期:2008-06-01 00:00:00

  • Chlorpromazine as permeabilizer and reagent for detection of microbial peroxidase and peroxidaselike activities.

    abstract::Chlorpromazine was used to perform a test for the detection of microbial peroxidase activities. The compound acts as both a cell permeabilizer and a reagent in the procedure developed which allows the detection of peroxidase and peroxidase like reactions both semiquantitatively in whole cell determinations and quantit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.6.1433-1435.1986

    authors: Galeazzi L,Turchetti G,Grilli G,Groppa G,Giunta S

    更新日期:1986-12-01 00:00:00

  • Citrate, a specific substrate for the isolation of Clostridium sphenoides.

    abstract::With a medium containing citrate as the carbon and energy source, 10 clostridial strains were isolated from various mud samples. Characterization of these strains revealed that they all belonged to the same species, Clostridium sphenoides. Strains of this organism obtained from culture collections were also able to gr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.4.955-962.1977

    authors: Walther R,Hippe H,Gottschalk G

    更新日期:1977-04-01 00:00:00

  • Isolation and Characterization of Marine Caulobacters and Assessment of Their Potential for Genetic Experimentation.

    abstract::A total of 25 marine caulobacters were isolated from littoral marine sources. Several aspects of their physiology and morphology were examined, as well as their suitability for genetic manipulation in laboratory cultivation. Caulobacters were readily isolated from all sources, including samples from areas containing p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.3.809-817.1988

    authors: Anast N,Smit J

    更新日期:1988-03-01 00:00:00

  • Starvation-Induced Changes in Motility, Chemotaxis, and Flagellation of Rhizobium meliloti

    abstract::The changes in motility, chemotactic responsiveness, and flagellation of Rhizobium meliloti RMB7201, L5-30, and JJ1c10 were analyzed after transfer of the bacteria to buffer with no available C, N, or phosphate. Cells of these three strains remained viable for weeks after transfer to starvation buffer (SB) but lost al...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.5.1708-1714.1998

    authors: Wei X,Bauer WD

    更新日期:1998-05-01 00:00:00

  • In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824.

    abstract::The restriction endonuclease Cac824I has been shown to be a major barrier to electrotransformation of Clostridium acetobutylicum ATCC 824 (L. D. Mermelstein, N. E. Welker, G. N. Bennett, and E. T. Papoutsakis, Bio/Technology 10:190-195, 1992). Methylation by the phi 3T I methyltransferase encoded by Bacillus subtilis ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.4.1077-1081.1993

    authors: Mermelstein LD,Papoutsakis ET

    更新日期:1993-04-01 00:00:00

  • Transformation of Rhodococcus fascians by High-Voltage Electroporation and Development of R. fascians Cloning Vectors.

    abstract::The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.9.2818-2825.1990

    authors: Desomer J,Dhaese P,Montagu MV

    更新日期:1990-09-01 00:00:00

  • Comparative analysis of antimicrobial activities of valinomycin and cereulide, the Bacillus cereus emetic toxin.

    abstract::Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02671-10

    authors: Tempelaars MH,Rodrigues S,Abee T

    更新日期:2011-04-01 00:00:00

  • Ecology of Vibrio parahaemolyticus and Vibrio vulnificus in the coastal and estuarine waters of Louisiana, Maryland, Mississippi, and Washington (United States).

    abstract::Vibrio parahaemolyticus and Vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea surface ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01296-12

    authors: Johnson CN,Bowers JC,Griffitt KJ,Molina V,Clostio RW,Pei S,Laws E,Paranjpye RN,Strom MS,Chen A,Hasan NA,Huq A,Noriea NF 3rd,Grimes DJ,Colwell RR

    更新日期:2012-10-01 00:00:00

  • Degradation of n-haloalkanes and alpha, omega-dihaloalkanes by wild-type and mutants of Acinetobacter sp. strain GJ70.

    abstract::A 1,6-dichlorohexane-degrading strain of Acinetobacter sp. was isolated from activated sludge. The organism could grow with and quantitatively release halide from 1,6-dichlorohexane, 1,9-dichlorononane, 1-chloropentane, 1-chlorobutane, 1-bromopentane, ethylbromide, and 1-iodopropane. Crude extracts contained an induci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.3.561-566.1987

    authors: Janssen DB,Jager D,Witholt B

    更新日期:1987-03-01 00:00:00

  • Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions.

    abstract::The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.11.3967-3971.1995

    authors: Platteeuw C,Hugenholtz J,Starrenburg M,van Alen-Boerrigter I,de Vos WM

    更新日期:1995-11-01 00:00:00

  • Temporal and geographical distributions of epilithic sodium dodecyl sulfate-degrading bacteria in a polluted South Wales river.

    abstract::Epilithic bacteria were isolated nonselectively from riverbed stones and examined by gel zymography for their ability to produce alkylsulfatase (AS) enzymes and thus to metabolize alkyl sulfate surfactants such as sodium dodecyl sulfate. The percentages of AS+ isolates from stone epilithon at five sites from the sourc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.2.555-560.1988

    authors: Anderson DJ,Day MJ,Russell NJ,White GF

    更新日期:1988-02-01 00:00:00

  • Stress-Induced Evolution of Heat Resistance and Resuscitation Speed in Escherichia coli O157:H7 ATCC 43888.

    abstract::The development of resistance in foodborne pathogens to food preservation techniques is an issue of increasing concern, especially in minimally processed foods where safety relies on hurdle technology. In this context, mild heat can be used in combination with so-called nonthermal processes, such as high hydrostatic p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02027-16

    authors: Gayán E,Cambré A,Michiels CW,Aertsen A

    更新日期:2016-10-27 00:00:00

  • Kinetics of methyl t-butyl ether cometabolism at low concentrations by pure cultures of butane-degrading bacteria.

    abstract::Butane-oxidizing Arthrobacter (ATCC 27778) bacteria were shown to degrade low concentrations of methyl t-butyl ether (MTBE; range, 100 to 800 microg/liter) with an apparent half-saturation concentration (K(s)) of 2.14 mg/liter and a maximum substrate utilization rate (k(c)) of 0.43 mg/mg of total suspended solids per ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.5.2197-2201.2001

    authors: Liu CY,Speitel GE Jr,Georgiou G

    更新日期:2001-05-01 00:00:00

  • Nickel promotes biofilm formation by Escherichia coli K-12 strains that produce curli.

    abstract::The survival of bacteria exposed to toxic compounds is a multifactorial phenomenon, involving well-known molecular mechanisms of resistance but also less-well-understood mechanisms of tolerance that need to be clarified. In particular, the contribution of biofilm formation to survival in the presence of toxic compound...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02171-08

    authors: Perrin C,Briandet R,Jubelin G,Lejeune P,Mandrand-Berthelot MA,Rodrigue A,Dorel C

    更新日期:2009-03-01 00:00:00

  • Prevalence of Borrelia burgdorferi sensu lato genospecies in Ixodes ricinus ticks in Europe: a metaanalysis.

    abstract::In Europe, Borrelia burgdorferi genospecies causing Lyme borreliosis are mainly transmitted by the tick Ixodes ricinus. Since its discovery, B. burgdorferi has been the subject of many epidemiological studies to determine its prevalence and the distribution of the different genospecies in ticks. In the current study w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,meta分析

    doi:10.1128/AEM.71.11.7203-7216.2005

    authors: Rauter C,Hartung T

    更新日期:2005-11-01 00:00:00

  • Characterization of phiLC3, a Lactococcus lactis subsp. cremoris temperature bacteriophage with cohesive single-stranded DNA ends.

    abstract::The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.11.3206-3211.1991

    authors: Lillehaug D,Lindqvist B,Birkeland NK

    更新日期:1991-11-01 00:00:00

  • Yeast communities of diverse Drosophila species: comparison of two symbiont groups in the same hosts.

    abstract::The combination of ecological diversity with genetic and experimental tractability makes Drosophila a powerful model for the study of animal-associated microbial communities. Despite the known importance of yeasts in Drosophila physiology, behavior, and fitness, most recent work has focused on Drosophila-bacterial int...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01741-12

    authors: Chandler JA,Eisen JA,Kopp A

    更新日期:2012-10-01 00:00:00