In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824.

abstract：:Clostridium-botulinum type A and type B spores were stored in tomato juice (pH 4.2) and citric acid-phosphate buffer (pH 4.2) at 4, 22, and 32 degrees C for 180 days. The spore count was determined at different intervals over the 180-day storage period. There was no significant decrease in the number of type A spores ...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Pflug IJ

更新日期：1977-07-01 00:00:00

abstract：:An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the ami...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Odaka A,Watanabe Y,Momose H

更新日期：1995-01-01 00:00:00

abstract：:A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, respectively). The sp...

journal_title：Applied and environmental microbiology

authors： Heuer H,Krsek M,Baker P,Smalla K,Wellington EM

更新日期：1997-08-01 00:00:00

abstract：:Kinetic parameters and the role of cytochrome c(3) in sulfate, Fe(III), and U(VI) reduction were investigated in Desulfovibrio vulgaris Hildenborough. While sulfate reduction followed Michaelis-Menten kinetics (K(m) = 220 micro M), loss of Fe(III) and U(VI) was first-order at all concentrations tested. Initial reducti...

journal_title：Applied and environmental microbiology

authors： Elias DA,Suflita JM,McInerney MJ,Krumholz LR

更新日期：2004-01-01 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:We have cloned the homoserine dehydrogenase genes (hom) from the gram-negative obligate methylotrophs Methylobacillus glycogenes ATCC 21276 and ATCC 21371 by complementation of an Escherichia coli homoserine dehydrogenase-deficient mutant. The 4.15-kb DNA fragment cloned from M. glycogenes ATCC 21371 also complemented...

journal_title：Applied and environmental microbiology

authors： Motoyama H,Maki K,Anazawa H,Ishino S,Teshiba S

更新日期：1994-01-01 00:00:00

abstract：:Cellulosilyticum ruminicola H1 is a newly described bacterium isolated from yak (Bos grunniens) rumen and is characterized by its ability to grow on a variety of hemicelluloses and degrade cellulosic materials. In this study, we performed the whole-genome sequencing of C. ruminicola H1 and observed a comprehensive set...

journal_title：Applied and environmental microbiology

authors： Cai S,Li J,Hu FZ,Zhang K,Luo Y,Janto B,Boissy R,Ehrlich G,Dong X

更新日期：2010-06-01 00:00:00

abstract：:While filamentous cyanobacteria play a crucial role in food web dynamics and biogeochemical cycling of many aquatic ecosystems around the globe, the knowledge regarding the phages infecting them is limited. Here, we describe the complete genome of the virulent cyanophage vB_AphaS-CL131 (here, CL 131), a Siphoviridae p...

journal_title：Applied and environmental microbiology

authors： Šulčius S,Šimoliūnas E,Alzbutas G,Gasiūnas G,Jauniškis V,Kuznecova J,Miettinen S,Nilsson E,Meškys R,Roine E,Paškauskas R,Holmfeldt K

更新日期：2018-12-13 00:00:00

abstract：:Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of eac...

journal_title：Applied and environmental microbiology

authors： Ishihara K,Shimokubo N,Sakagami A,Ueno H,Muramatsu Y,Kadosawa T,Yanagisawa C,Hanaki H,Nakajima C,Suzuki Y,Tamura Y

更新日期：2010-08-01 00:00:00

abstract：:A rapid and simple approach to the small-subunit (SSU) rRNA-based quantitative detection of a specific group of microorganisms in complex ecosystems has been developed. The method employs sequence-specific cleavage of rRNA molecules with oligonucleotides and RNase H. Defined mixtures of SSU rRNAs were mixed with an ol...

journal_title：Applied and environmental microbiology

authors： Uyeno Y,Sekiguchi Y,Sunaga A,Yoshida H,Kamagata Y

更新日期：2004-06-01 00:00:00

abstract：:The LlaDII restriction/modification (R/M) system was found on the naturally occurring 8.9-kb plasmid pHW393 in Lactococcus lactis subsp. cremoris W39. A 2.4-kb PstI-EcoRI fragment inserted into the Escherichia coli-L. lactis shuttle vector pCI3340 conferred to L. lactis LM2301 and L. lactis SMQ86 resistance against re...

journal_title：Applied and environmental microbiology

authors： Madsen A,Josephsen J

更新日期：1998-07-01 00:00:00

abstract：:Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of t...

journal_title：Applied and environmental microbiology

authors： Kästner M,Breuer-Jammali M,Mahro B

更新日期：1998-01-01 00:00:00

abstract：:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blen...

journal_title：Applied and environmental microbiology

authors： Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

更新日期：1990-06-01 00:00:00

abstract：:To define conditions promoting inherited infection by Lyme disease spirochetes in Ixodes ticks, we variously infected ticks with Borrelia afzelii and examined their progenies by dark-field microscopy, immunofluorescence, PCR, and serial passage. No episode of inherited infection was evident, regardless of instar or ge...

journal_title：Applied and environmental microbiology

authors： Matuschka FR,Schinkel TW,Klug B,Spielman A,Richter D

更新日期：1998-08-01 00:00:00

abstract：:Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in ma...

journal_title：Applied and environmental microbiology

authors： Chujo M,Yoshida S,Ota A,Murata K,Kawai S

更新日期：2015-01-01 00:00:00

abstract：:Ten laboratories participated in an interlaboratory comparison of determination of bioleaching rates of a pyrite reference material. A standardized procedure and a single strain of Thiobacillus ferrooxidans were used in this study. The mean rate of bioleaching of the pyrite reference material was 12.4 mg of Fe per lit...

journal_title：Applied and environmental microbiology

authors： Olson GJ

更新日期：1991-03-01 00:00:00

abstract：:Bacterioplankton are fundamental components of marine ecosystems and influence the entire biosphere by contributing to the global biogeochemical cycles of key elements. Yet, there is a significant gap in knowledge about their diversity and specific activities, as well as environmental factors that shape their communit...

journal_title：Applied and environmental microbiology

authors： Richa K,Balestra C,Piredda R,Benes V,Borra M,Passarelli A,Margiotta F,Saggiomo M,Biffali E,Sanges R,Scanlan DJ,Casotti R

更新日期：2017-08-17 00:00:00

abstract：:The fate of Listeria monocytogenes during refrigerated storage was determined on several processed meat products, including ham, bologna, wieners, sliced chicken, sliced turkey, fermented semidried sausage, bratwurst, and cooked roast beef. The meats were surface inoculated with a five-strain mixture of less than or e...

journal_title：Applied and environmental microbiology

authors： Glass KA,Doyle MP

更新日期：1989-06-01 00:00:00

abstract：:Twenty fungal genera, including 14 Fusarium species, were examined for ice nucleation activity at -5.0 degrees C, and this activity was found only in Fusarium acuminatum and Fusarium avenaceum. This characteristic is unique to these two species. Ice nucleation activity of F. avenaceum was compared with ice nucleation ...

journal_title：Applied and environmental microbiology

authors： Pouleur S,Richard C,Martin JG,Antoun H

更新日期：1992-09-01 00:00:00

abstract：:In a field study of 29 dairy farms, Pseudomonas aeruginosa was isolated more frequently (P = 0.05) from milking parlor udder wash water systems containing iodophor germicides than from those with no germicide. Most available iodine (AI2) concentrations were below the recommended level of 25 ppm (25 microgram/ml). Rubb...

journal_title：Applied and environmental microbiology

authors： Hicks CR,Eberhart RJ,Erskine RJ,Spencer SB

更新日期：1991-02-01 00:00:00

abstract：:Although carbon dioxide (CO(2)) is known to inhibit growth of most bacteria, very little is known about the cellular response. The food-borne pathogen Listeria monocytogenes is characterized by its ability to grow in high CO(2) concentrations at refrigeration temperatures. We examined the listerial responses of differ...

journal_title：Applied and environmental microbiology

authors： Jydegaard-Axelsen AM,Høiby PE,Holmstrøm K,Russell N,Knøchel S

更新日期：2004-07-01 00:00:00

abstract：:A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servic...

journal_title：Applied and environmental microbiology

authors： La Duc MT,Osman S,Vaishampayan P,Piceno Y,Andersen G,Spry JA,Venkateswaran K

更新日期：2009-10-01 00:00:00

abstract：:A selective procedure was used to isolate pectinolytic intestinal bacteria from human subjects. The three isolates with the greatest pectinolytic activity utilized pectin and a few related compounds as fermentable substrates for growth but did not utilize any other compound tested. Thus, their substrate utilization pa...

journal_title：Applied and environmental microbiology

authors： Jensen NS,Canale-Parola E

更新日期：1985-07-01 00:00:00

abstract：:The gut microbiota is a diverse and dynamic ecological community that is increasingly recognized to play important roles in host metabolic, immunological, and behavioral functioning. As such, identifying new routes for manipulating the microbiota may provide valuable additional methods for improving host health. Dieta...

journal_title：Applied and environmental microbiology

authors： Reese AT,Carmody RN

更新日期：2019-05-02 00:00:00

abstract：:The susceptibility of a strain of Legionella pneumophila to disinfection by an organic halamine, free chlorine, and a mixture of the organic halamine and free chlorine was assessed. The organic halamine was found to have superior stability in solution and to exhibit adequate disinfectant potential over a period of 1 m...

journal_title：Applied and environmental microbiology

authors： Swango LJ,Wilt GR,Killen AD,Williams DE,Worley SD

更新日期：1987-12-01 00:00:00

abstract：:Understanding how species interaction and assembly processes structure the abundant and rare bacterial biospheres in soils is crucial for predicting how biodiversity influences ecosystem functioning. Here, we profiled the bacterial communities across a salt marsh ecosystem gradient to investigate the co-occurrence pat...

journal_title：Applied and environmental microbiology

authors： Du S,Dini-Andreote F,Zhang N,Liang C,Yao Z,Zhang H,Zhang D

更新日期：2020-06-17 00:00:00

abstract：:Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl(2) under a wide range of Na...

journal_title：Applied and environmental microbiology

authors： Sharma PK,Balkwill DL,Frenkel A,Vairavamurthy MA

更新日期：2000-07-01 00:00:00

abstract：:Potato tuber disks were submerged in suspensions containing 10 to 10 cells of Agrobacterium tumefaciens B6 per ml. After 60 min, the disks were rinsed and homogenized, and portions of the homogenates were plated to measure the number of adsorbed bacteria. At low initial bacterial concentrations (10/ml), 5 to 23% of th...

journal_title：Applied and environmental microbiology

authors： Kluepfel DA,Pueppke SG

更新日期：1985-06-01 00:00:00

abstract：:The reliable detection of airborne biological threat agents depends on several factors, including the performance criteria of the detector and its operational environment. One step in improving the detector's performance is to increase our knowledge of the biological aerosol background in potential operational environ...

journal_title：Applied and environmental microbiology

authors： Dybwad M,Granum PE,Bruheim P,Blatny JM

更新日期：2012-03-01 00:00:00

abstract：:We have analyzed the impact of surface-to-volume ratio on final bacterial concentrations after batch growth. We examined six bottle sizes (20 to 1,000 ml) using three independent enumeration methods to quantify growth. We found no evidence of a so-called volumetric bottle effect, thus contradicting numerous previous r...

journal_title：Applied and environmental microbiology

authors： Hammes F,Vital M,Egli T

更新日期：2010-02-01 00:00:00