Abstract:
:Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in mannitol-containing medium. Based on microarray data, complementation analysis, and cell growth data, we demonstrated that acquisition of mannitol-assimilating ability was due to spontaneous mutations in the genes encoding Tup1 or Cyc8, which constitute a general corepressor complex that regulates many kinds of genes. We also showed that an S. cerevisiae strain carrying a mutant allele of CYC8 exhibited superior salt tolerance relative to other ethanologenic microorganisms; this characteristic would be highly beneficial for the production of bioethanol from marine biomass. Thus, we succeeded in conferring the ability to assimilate mannitol on S. cerevisiae through dysfunction of Tup1-Cyc8, facilitating production of ethanol from mannitol.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Chujo M,Yoshida S,Ota A,Murata K,Kawai Sdoi
10.1128/AEM.02906-14subject
Has Abstractpub_date
2015-01-01 00:00:00pages
9-16issue
1eissn
0099-2240issn
1098-5336pii
AEM.02906-14journal_volume
81pub_type
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