Development of a random amplification of polymorphic DNA typing method for Listeria monocytogenes.

abstract：:The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjug...

journal_title：Applied and environmental microbiology

authors： Musovic S,Oregaard G,Kroer N,Sørensen SJ

更新日期：2006-10-01 00:00:00

abstract：:The genetic diversity of Desulfovibrio species in environmental samples was determined by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified [NiFe] hydrogenase gene fragments. Five different PCR primers were designed after comparative analysis of [NiFe] hydrogenase gene sequences from three Desulfovibrio ...

journal_title：Applied and environmental microbiology

authors： Wawer C,Muyzer G

更新日期：1995-06-01 00:00:00

abstract：:A methylomonad culture was isolated from pond water and examined as a potential source of single-cell protein. A medium containing magnesium sulfate, ammonium hydroxide, sodium phosphate, tap water, and methanol supported the growth of the isolate. Optimal growth conditions in batch cultures for the organism were: tem...

journal_title：Applied and environmental microbiology

authors： Chen BJ,Hirt W,Lim HC,Tsao GT

更新日期：1977-02-01 00:00:00

abstract：:Bacterial degradation of biphenyl and polychlorinated biphenyls proceeds by a well-studied pathway which produces benzoate and 2-hydroxypent-2,4-dienoate (or, in the case of polychlorinated biphenyls, the chlorinated derivatives of these compounds). Pseudomonas cepacia P166 utilizes 4-chlorobiphenyl for growth and pro...

journal_title：Applied and environmental microbiology

authors： Arensdorf JJ,Focht DD

更新日期：1995-02-01 00:00:00

abstract：:The spirochete Borrelia burgdorferi is the causative agent of Lyme disease, the leading vector-borne illness in the United States. Many of the genetic factors affecting spirochete morphology and physiology are unknown due to the limited genetic tools available and the large number of open reading frames with unknown f...

journal_title：Applied and environmental microbiology

authors： Stewart PE,Hoff J,Fischer E,Krum JG,Rosa PA

更新日期：2004-10-01 00:00:00

abstract：:Dibenzothiophene is degraded to 3-hydroxy-2-formyl benzothiophene by various bacteria, including a strain of Pseudomonas putida that also forms dibenzothiophene sulfone via an alternate pathway. By using these end products as substrates, mixed enrichment cultures that could degrade 3-hydroxy-2-formyl benzothiophene an...

journal_title：Applied and environmental microbiology

authors： Mormile MR,Atlas RM

更新日期：1988-12-01 00:00:00

abstract：:The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...

journal_title：Applied and environmental microbiology

authors： Martinez-Medina M,Garcia-Gil J,Barnich N,Wieler LH,Ewers C

更新日期：2011-08-15 00:00:00

abstract：:Extraintestinal growth of fecal bacteria can impair accurate assessment of watershed health. Anaerobic fecal bacteria belonging to the order Bacteroidales are attractive candidates for fecal source tracking because they have host-specific distributions and do not grow well in the presence of high oxygen concentrations...

journal_title：Applied and environmental microbiology

authors： Walters SP,Field KG

更新日期：2006-07-01 00:00:00

abstract：:The effects of ethyl picolinate on germination, outgrowth, and sporulation of Bacillus cereus T were studied in a synthetic medium containing glucose. Ethyl picolinate specifically inhibited at two stages, outgrowth and sporulation. The initiation of germination and cell division was not affected. The inhibition of ou...

journal_title：Applied and environmental microbiology

authors： Pandey NK,Gollakota KG

更新日期：1977-05-01 00:00:00

abstract：:A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures....

journal_title：Applied and environmental microbiology

authors： Loessner MJ,Schneider A,Scherer S

更新日期：1995-03-01 00:00:00

abstract：:ω-Transaminase (ω-TA) is a promising enzyme for use in the production of unnatural amino acids from keto acids using cheap amino donors such as isopropylamine. The small substrate-binding pocket of most ω-TAs permits entry of substituents no larger than an ethyl group, which presents a significant challenge to the pre...

journal_title：Applied and environmental microbiology

authors： Han SW,Park ES,Dong JY,Shin JS

更新日期：2015-10-01 00:00:00

abstract：:We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL...

journal_title：Applied and environmental microbiology

authors： Joseph-Horne T,Hollomon D,Manning N,Kelly SL

更新日期：1996-01-01 00:00:00

abstract：:The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis, Nucleospin, and Dynabeads DNA Direct Sy...

journal_title：Applied and environmental microbiology

authors： De Medici D,Croci L,Delibato E,Di Pasquale S,Filetici E,Toti L

更新日期：2003-06-01 00:00:00

abstract：:A strain of the aerobic anoxygenic photosynthetic bacteria was isolated from a deep-ocean hydrothermal vent plume environment. The in vivo absorption spectra of cells indicate the presence of bacteriochlorophyll a incorporated into light-harvesting complex I and a reaction center. The general morphological and physiol...

journal_title：Applied and environmental microbiology

authors： Yurkov V,Beatty JT

更新日期：1998-01-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:Recent declines in bumble bee populations are of great concern and have prompted critical evaluations of the role of pathogen introductions and host resistance in bee health. One factor that may influence host resilience when facing infection is the gut microbiota. Previous experiments with Bombus terrestris, a Europe...

journal_title：Applied and environmental microbiology

authors： Mockler BK,Kwong WK,Moran NA,Koch H

更新日期：2018-03-19 00:00:00

abstract：:[This corrects the article on p. 662 in vol. 45.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-06-01 00:00:00

abstract：:Lactic acid bacteria have a good potential as agents for the delivery of heterologous proteins to the gastrointestinal mucosa and thus for the reequilibration of inappropriate immune responses to food antigens. Bovine beta-lactoglobulin (BLG) is considered a major allergen in cow's milk allergy. We have designed recom...

journal_title：Applied and environmental microbiology

authors： Bernasconi E,Germond JE,Delley M,Fritsché R,Corthésy B

更新日期：2002-06-01 00:00:00

abstract：:To understand the ecological and genetic role of viruses in the marine environment, it is critical to know the infectivity of viruses and the types of interactions that occur between marine viruses and their hosts. We isolated four marine phages from turbid plaques by using four indigenous bacterial hosts obtained fro...

journal_title：Applied and environmental microbiology

authors： Jiang SC,Kellogg CA,Paul JH

更新日期：1998-02-01 00:00:00

abstract：:Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

journal_title：Applied and environmental microbiology

authors： Gandhi KM,Mandrell RE,Tian P

更新日期：2010-12-01 00:00:00

abstract：:Pantoea vagans C9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of Erwinia amylovora, the causal agent of fire blight disease of pear and apple. One antibiotic, herbicolin I, was purified from culture filtrates of P. vagans C9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarb...

journal_title：Applied and environmental microbiology

authors： Kamber T,Lansdell TA,Stockwell VO,Ishimaru CA,Smits TH,Duffy B

更新日期：2012-06-01 00:00:00

abstract：:Graded doses of pure ochratoxin A (0, 0.5, 1.0, 2.0, 4.0, and 8.0 microgram of toxin per g of feed) were incorporated into a commercial diet which was fed to chickens from hatching to 3 weeks of age, at which time the experiments were terminated. Liver glycogen levels were elevated significantly (P less than 0.05) by ...

journal_title：Applied and environmental microbiology

authors： Huff WE,Doerr JA,Hamilton PB

更新日期：1979-01-01 00:00:00

abstract：:Analysis of known sulfur oxygenase-reductases (SORs) and the SOR-like sequences identified from public databases indicated that they all possess three cysteine residues within two conserved motifs (V-G-P-K-V-C(31) and C(101)-X-X-C(104); numbering according to the Acidianus tengchongensis numbering system). The thio-mo...

journal_title：Applied and environmental microbiology

authors： Chen ZW,Jiang CY,She Q,Liu SJ,Zhou PJ

更新日期：2005-02-01 00:00:00

abstract：:Epimerization of sugar nucleotides is central to the structural diversification of monosaccharide building blocks for cellular biosynthesis. Epimerase applicability to carbohydrate synthesis can be limited, however, by the high degree of substrate specificity exhibited by most sugar nucleotide epimerases. Here, we dis...

journal_title：Applied and environmental microbiology

authors： Rapp C,van Overtveldt S,Beerens K,Weber H,Desmet T,Nidetzky B

更新日期：2020-12-04 00:00:00

abstract：:Acinetobacter sp. strain P6 has previously been reported to utilize biphenyl (BP) and chlorinated BPs, with accumulation of corresponding chlorobenzoic acids. Arthrobacter sp. strain M5 was isolated as a contaminant in the culture of Acinetobacter sp. strain P6 growing on 4-chlorobiphenyl and showed properties similar...

journal_title：Applied and environmental microbiology

authors： Furukawa K,Chakrabarty AM

更新日期：1982-09-01 00:00:00

abstract：:Anthrax is a fatal infection of humans and livestock that is caused by the gram-positive bacterium Bacillus anthracis. The virulent strains of B. anthracis are encapsulated and toxigenic. In this paper we describe the development of a PCR technique for identifying spores of B. anthracis. Two 20-mer oligonucleotide pri...

journal_title：Applied and environmental microbiology

authors： Reif TC,Johns M,Pillai SD,Carl M

更新日期：1994-05-01 00:00:00

abstract：:The wastewater of livestock slaughterhouses is considered a source of antimicrobial-resistant bacteria with clinical relevance and may thus be important for their dissemination into the environment. To get an overview of their occurrence and characteristics, we investigated process water (n = 50) from delivery and unc...

journal_title：Applied and environmental microbiology

authors： Savin M,Bierbaum G,Hammerl JA,Heinemann C,Parcina M,Sib E,Voigt A,Kreyenschmidt J

更新日期：2020-04-01 00:00:00

abstract：:Seawater enrichments of marine bacteria clustered in 20- to 50-(mu)m-wide bands near air-water interfaces. The cells within the band travelled at up to 212 (mu)m s(sup-1) and at an average speed of 163 (mu)m s(sup-1). Mean cell speeds peaked mid-run at 187 (mu)m s(sup-1). At the end of the run, bacteria reversed direc...

journal_title：Applied and environmental microbiology

authors： Mitchell JG,Pearson L,Dillon S

更新日期：1996-10-01 00:00:00

abstract：:Chitin is massively produced by freshwater plankton species as a structural element of their exoskeleton or cell wall. At the same time, chitin does not accumulate in the predominantly anoxic sediments, underlining its importance as carbon and nitrogen sources for sedimentary microorganisms. We studied chitin degradat...

journal_title：Applied and environmental microbiology

authors： Wörner S,Pester M

更新日期：2019-08-29 00:00:00

abstract：:Soil bacteria adapt to diverse and rapidly changing environmental conditions by sensing and responding to environmental cues using a variety of sensory systems. Two-component systems are a widespread type of signal transduction system present in all three domains of life and typically are comprised of a sensor kinase ...

journal_title：Applied and environmental microbiology

authors： Luu RA,Schomer RA,Brunton CN,Truong R,Ta AP,Tan WA,Parales JV,Wang YJ,Huo YW,Liu SJ,Ditty JL,Stewart V,Parales RE

更新日期：2019-10-30 00:00:00