Abstract:
:The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16S rRNA genes from the sorted cells. The host range of the pKJK10 plasmid was exceptionally broad, as it included not only bacteria belonging to the alpha, beta, and gamma subclasses of the Proteobacteria, but also Arthrobacter sp., a gram-positive member of the Actinobacteria. The transfer frequency (transconjugants per donor) from the Pseudomonas putida donor to the indigenous bacteria was 7.03 x 10(-2) +/- 3.84 x 10(-2). This is the first direct documentation of conjugal transfer between gram-negative donor and gram-positive recipient bacteria in situ.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Musovic S,Oregaard G,Kroer N,Sørensen SJdoi
10.1128/AEM.00013-06subject
Has Abstractpub_date
2006-10-01 00:00:00pages
6687-92issue
10eissn
0099-2240issn
1098-5336pii
72/10/6687journal_volume
72pub_type
杂志文章abstract::The need for protozoa for the proliferation of Legionella pneumophila in aquatic habitats is still not fully understood and is even questioned by some investigators. This study shows the in vivo growth of L. pneumophila in protozoa in aquatic biofilms developing at high concentrations on plasticized polyvinyl chloride...
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pub_type: 杂志文章
doi:10.1128/AEM.70.11.6826-6833.2004
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2005-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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更新日期:1979-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00