Abstract:
:A recombinant Escherichia coli strain carrying a plasmid with an antibiotic resistance marker and expressing the green fluorescent protein was inoculated at a concentration of 3.8 x 10(8) CFU/g into direct-cut wheat (348 g of dry matter kg(-1)), wilted wheat (450 g of dry matter kg(-1)), and corn (375 g of dry matter kg(-1)). The forages were ensiled in mini-silos. The treatments included control (no E. coli added), application of tagged E. coli, and delayed sealing of the inoculated wheat. Three silos per treatment were sampled on predetermined dates, and the numbers of E. coli were determined on Chromocult TBX medium with or without kanamycin. Colonies presumptively identified as E. coli were also tested for fluorescence activity. Addition of E. coli at the time of ensiling resulted in a more rapid decrease in the pH but had almost no effect on the chemical composition of the final silages or their aerobic stability. E. coli disappeared from the silages when the pH decreased below 5.0. It persisted longer in silages of wilted wheat, in which the pH declined more slowly. Control silages of all crops also contained bacteria, presumptively identified as E. coli, that were resistant to the antibiotic, which suggests that some epiphytic strains are naturally resistant to antibiotics.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Chen Y,Sela S,Gamburg M,Pinto R,Weinberg ZGdoi
10.1128/AEM.71.9.5163-5170.2005keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
5163-70issue
9eissn
0099-2240issn
1098-5336pii
71/9/5163journal_volume
71pub_type
杂志文章abstract::Cyanuric acid, a metabolic intermediate in the degradation of many s-triazine compounds, is further metabolized by cyanuric acid hydrolase. Cyanuric acid also accumulates in swimming pools due to the breakdown of the sanitizing agents di- and trichloroisocyanuric acid. Structurally stable cyanuric acid hydrolases are ...
journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.10.3117-3124.1990
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abstract::Bacterial isolates from frogs were phenotypically identified as Ochrobactrum anthropi, but 16S rRNA sequencing showed up to 100% identity with Brucella inopinata. Further analysis of recA, omp2a, omp2b, bcsp31, and IS711 and multilocus sequence analysis (MLSA) verified a close relationship with Brucella, suggesting th...
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doi:10.1128/AEM.61.4.1666-1668.1995
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doi:10.1128/AEM.64.10.3854-3859.1998
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.63.1.324-328.1997
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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doi:10.1128/aem.66.4.1280-1285.2000
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journal_title:Applied and environmental microbiology
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doi:
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.71.6.3041-3048.2005
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journal_title:Applied and environmental microbiology
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更新日期:1983-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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更新日期:1978-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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更新日期:1984-05-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2012-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.10.3648-3655.1998
更新日期:1998-10-01 00:00:00
abstract::We investigated the growth parameters of Saccharomyces rouxii isolated from spoiled chocolate syrup. The optimum pH range for S. rouxii was 3.5 to 5.5, whereas the minimum and maximum pH values that permitted growth were 1.5 and 10.5, respectively. For cells grown in 0 and 60% sucrose the optimum water activity (aw) v...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.5.1614-1621.1983
更新日期:1983-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.7.2259-2261.1990
更新日期:1990-07-01 00:00:00