Abstract:
:The deoxyribonucleic acid (DNA) yield and deoxyribonuclease (DNase) activity of several yeasts were correlated. Debaryomyces castellii and Debaryomyces franciscae were found to contain active DNases which carry out DNA hydrolysis, whereas the amounts of DNA as determined by extraction with Sarkosyl buffer (pH 7.8) were found to be small. On the other hand, Candida parapsilosis, Saccharomyces carmosousae, and Lodderomyces elongisporus produced no detectable DNases active at pH 7.8, and their DNA yield was correspondingly high. L. elongisporus was found to possess DNase only at pH 4.0.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
El-Nakhal H,Phaff HJdoi
10.1128/AEM.41.6.1482-1483.1981keywords:
subject
Has Abstractpub_date
1981-06-01 00:00:00pages
1482-3issue
6eissn
0099-2240issn
1098-5336journal_volume
41pub_type
杂志文章abstract::To enhance the production of isoprene, a volatile 5-carbon hydrocarbon, in the Gram-positive spore-forming rod-shaped bacterium Bacillus subtilis, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and 1-deoxy-d-xylulose-5-phosphate reductoisomerase (Dxr) were overexpressed in B. subtilis DSM 10. For the strain that overex...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02341-10
更新日期:2011-04-01 00:00:00
abstract::The effect of the glutathione reductase (GshR) activity of Lactobacillus sanfranciscensis DSM20451(T) on the thiol levels in fermented sourdoughs was determined, and the oxygen tolerance of the strain was also determined. The gshR gene coding for a putative GshR was sequenced and inactivated by single-crossover integr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02322-06
更新日期:2007-07-01 00:00:00
abstract::A heat-stable extracellular protease from Pseudomonas fluorescens was purified by chromatography on a DEAE-cellulose column and gel filtration on a Sephadex G100 column. The homogeneous enzyme preparation was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relatedness of prot...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.1.6-12.1983
更新日期:1983-07-01 00:00:00
abstract::The reliable detection of airborne biological threat agents depends on several factors, including the performance criteria of the detector and its operational environment. One step in improving the detector's performance is to increase our knowledge of the biological aerosol background in potential operational environ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.07212-11
更新日期:2012-03-01 00:00:00
abstract::We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and even...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02835-08
更新日期:2009-07-01 00:00:00
abstract::Variations in the thymidine incorporation rate, bacterial abundance, and mean cell volumes in the surface water (0.5 m) of the Baltic Sea in spring and summer were compared in studies with different spatial scales (570 nautical miles [nmi] [ca. 1056 km], 220 nmi [ca. 407 km], 24 nmi [ca. 44 km], 12 nmi [ca. 22 km], an...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.11.3150-3155.1991
更新日期:1991-11-01 00:00:00
abstract::Traditional three-domain fungal and bacterial laccases have been extensively studied for their significance in various biotechnological applications. Growing molecular evidence points to a wide occurrence of more recently recognized two-domain laccase-like multicopper oxidase (LMCO) genes in Streptomyces spp. However,...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00223-14
更新日期:2014-06-01 00:00:00
abstract::Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate in the cytoplasm. We conducted experimental evolution by daily diluting cultures in increasing concentrations of benzoic acid (up to 20 mM) buffered at external pH 6.5, a pH at which perm...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02736-16
更新日期:2016-12-30 00:00:00
abstract::Two Aeromonas hydrophila bacteriophages, Aeh1 and Aeh2, were isolated from sewage. Both phages showed binal symmetry. The dimensions of A. hydrophila phages Aeh1 and Aeh2 differed from those of the other Aeromonas phages. Also, phage Aeh2 was the largest Aeromonas phage studied to date. Phage Aeh1 formed small, clear ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.5.1670-1676.1983
更新日期:1983-05-01 00:00:00
abstract::Three predominant ruminal cellulolytic bacteria (Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and Ruminococcus albus 7) were grown in different binary combinations to determine the outcome of competition in either cellulose-excess batch culture or in cellulose-limited continuous culture. Relative popu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.2.734-742.1997
更新日期:1997-02-01 00:00:00
abstract::A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.5.1067-1071.1985
更新日期:1985-05-01 00:00:00
abstract::The design of fully or partly defined media for mycoplasma cultivation involves the need to provide the essential lipids, cholesterol and long-chain fatty acids, in an assimilable and nontoxic form. This study introduces cyclodextrins (CDs) as carriers of these lipids, thus suggesting alternatives to serum or bovine s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.2.547-551.1993
更新日期:1993-02-01 00:00:00
abstract::Coumarin concentrations that inhibited growth of Pythium sp. rapidly decreased the rate of incorporation of [14C]glucose into the mycelium. Coumarin also reduced drastically the carbohydrate and protein content of the cytoplasm and, to a lesser extent, the amino acid content and cell wall fraction. Enzymes related to ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.34.3.258-262.1977
更新日期:1977-09-01 00:00:00
abstract::Growth of Clostridium thermocellum in batch cultures was studied over a broad range of cellobiose concentrations. Cultures displayed important differences in their substrate metabolism as determined by the end product yields. Bacterial growth was severely limited when the initial cellobiose concentration was 0.2 (wt/v...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.5.1126-1129.1984
更新日期:1984-05-01 00:00:00
abstract::Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplificati...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02327-17
更新日期:2018-02-14 00:00:00
abstract::The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00686-10
更新日期:2010-08-01 00:00:00
abstract::GELRITE, a new gelling agent with good thermal stability and clarity, was evaluated in media for culturing selected thermophilic microorganisms. It was also evaluated for performing counts of thermophilic bacteria from soil samples. In most cases, GELRITE was shown to be superior to agar for these applications. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.2.427-429.1984
更新日期:1984-02-01 00:00:00
abstract::Most strains of Butyrivibrio fibrisolvens are known to produce extracellular polysaccharides (EPs). However, the rheological and functional properties of these EPs have not been determined. Initially, 26 strains of Butyrivibrio were screened for EP yield and apparent viscosities of cell-free supernatants. Yields range...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.7.2016-2020.1991
更新日期:1991-07-01 00:00:00
abstract::Sterol demethylation inhibitor (DMI) fungicides are widely used to control fungi pathogenic to humans and plants. Resistance to DMIs is mediated either through alterations in the structure of the target enzyme CYP51 (encoding 14alpha-demethylase), through increased expression of the CYP51 gene, or through increased ex...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.4.2581-2585.2006
更新日期:2006-04-01 00:00:00
abstract::Two new polyaromatic hydrocarbon-degrading marine bacteria have been isolated from burrow wall sediments of benthic macrofauna by using enrichments on phenanthrene. Strain LC8 (from a polychaete) and strain M4-6 (from a mollusc) are aerobic and gram negative and require sodium chloride (>1%) for growth. Both strains c...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.12.5585-5592.2001
更新日期:2001-12-01 00:00:00
abstract::Genome-wide analysis has revealed abundant FabG (beta-ketoacyl-ACP reductase) paralogs, with uncharacterized biological functions, in several halophilic archaea. In this study, we identified for the first time that the fabG1 gene, but not the other five fabG paralogs, encodes the polyhydroxyalkanoate (PHA)-specific ac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00938-09
更新日期:2009-10-01 00:00:00
abstract::Parasitic chytrid fungi can inflict significant mortality on cyanobacteria but frequently fail to keep cyanobacterial dominance and bloom formation in check. Our study tested whether oligopeptide production, a common feature in many cyanobacteria, can be a defensive mechanism against chytrid parasitism. The study empl...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03499-12
更新日期:2013-04-01 00:00:00
abstract::The conversion of ochratoxin C to ochratoxin A was studied in rats after oral and intravenous administration. The concentration of ochratoxin A in the blood as a function of time was the same after oral administration of equivalent amounts of either ochratoxin C or ochratoxin A. The maximum ochratoxin A concentrations...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.1.41-42.1984
更新日期:1984-07-01 00:00:00
abstract::The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and bio...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.3.1532-1541.2003
更新日期:2003-03-01 00:00:00
abstract::Plasmodium falciparum, in addition to scavenging essential fatty acids from its intra- and intercellular environments, possesses a functional complement of type II fatty acid synthase (FAS) enzymes targeted to the apicoplast organelle. Recent evidence suggests that products of the plasmodial FAS II system may be criti...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00170-10
更新日期:2010-06-01 00:00:00
abstract::Three species of saltmarsh ascomycetes were grown in the presence of all of the constituents of their natural substrate (leaves of cordgrass) or were presented only with aqueous extracts of the leaves. These two growth-condition treatments had no significant effect on total ergosterol content of the fungal mycelia, co...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.9.3479-3482.1994
更新日期:1994-09-01 00:00:00
abstract::Simian rotavirus SA-11 was concentrated from tap water by adsorption to and elution from microporous filters, followed by organic flocculation. Two types of filters were compared for their ability to concentrate the virus. Both Zeta Plus 60S and Cox AA type M-780 filters were efficient for virus adsorption, but the ef...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.3.850-855.1983
更新日期:1983-03-01 00:00:00
abstract::Members of candidate division OP11 are widely distributed in terrestrial and marine ecosystems, yet little information regarding their metabolic capabilities and ecological role within such habitats is currently available. Here, we report on the microfluidic isolation, multiple-displacement-amplification, pyrosequenci...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06059-11
更新日期:2011-11-01 00:00:00
abstract::Several selective media were evaluated for the primary isolation and enumeration of Staphylococcus aureus from halogenated indoor swimming pool waters. Standard plate counts of the viable population and total coliform densities were also determined to ascertain their value as indicator systems. All studies were done w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.4.699-702.1986
更新日期:1986-04-01 00:00:00
abstract::Wild-type Bacillus subtilis ferments 20 g/liter glucose in 48 h, producing lactate and butanediol, but not ethanol or acetate. To construct an ethanologenic B. subtilis strain, homologous recombination was used to disrupt the native lactate dehydrogenase (LDH) gene (ldh) by chromosomal insertion of the Zymomonas mobil...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00625-07
更新日期:2007-08-01 00:00:00