Identification of the polyhydroxyalkanoate (PHA)-specific acetoacetyl coenzyme A reductase among multiple FabG paralogs in Haloarcula hispanica and reconstruction of the PHA biosynthetic pathway in Haloferax volcanii.

abstract：:Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay w...

journal_title：Applied and environmental microbiology

authors： Geng T,Morgan MT,Bhunia AK

更新日期：2004-10-01 00:00:00

abstract：:The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus bre...

journal_title：Applied and environmental microbiology

authors： Macfarlane S,Woodmansey EJ,Macfarlane GT

更新日期：2005-11-01 00:00:00

abstract：:A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated that the mutant was deficient in d-xylose reductase activity that used NADPH exclusively as a cofactor, but not in activity...

journal_title：Applied and environmental microbiology

authors： Schneider H,Lee H,Barbosa Mde F,Kubicek CP,James AP

更新日期：1989-11-01 00:00:00

abstract：:The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...

journal_title：Applied and environmental microbiology

authors： Martinez-Medina M,Garcia-Gil J,Barnich N,Wieler LH,Ewers C

更新日期：2011-08-15 00:00:00

abstract：:The noncanonical alcohol dehydrogenase AlkJ is encoded on the alkane-metabolizing alk operon of the mesophilic bacterium Pseudomonas putida GPo1. To gain insight into the enzymology of AlkJ, we have produced the recombinant protein in Escherichia coli and purified it to homogeneity using His6 tag affinity and size exc...

journal_title：Applied and environmental microbiology

authors： Kirmair L,Skerra A

更新日期：2014-04-01 00:00:00

abstract：:The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

journal_title：Applied and environmental microbiology

authors： Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

更新日期：2012-10-01 00:00:00

abstract：:The use of Lactococcus lactis (the most extensively characterized lactic acid bacterium) as a delivery organism for heterologous proteins is, in some cases, limited by low production levels and poor-quality products due to surface proteolysis. In this study, we combined in one L. lactis strain use of the nisin-inducib...

journal_title：Applied and environmental microbiology

authors： Miyoshi A,Poquet I,Azevedo V,Commissaire J,Bermudez-Humaran L,Domakova E,Le Loir Y,Oliveira SC,Gruss A,Langella P

更新日期：2002-06-01 00:00:00

abstract：:The concentration of 2-aminoethylphosphonic acid has been measured in seven genera of rumen ciliate protozoa. Expressed as milligrams per gram of total nitrogen, 2-aminoethylphosphonic acid concentrations ranged from 17.2 in Ophryoscolex spp. to 72.4 in Eremoplastron spp. ...

journal_title：Applied and environmental microbiology

authors： Whitelaw FG,Bruce LA,Eadie JM,Shand WJ

更新日期：1983-10-01 00:00:00

abstract：:Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of t...

journal_title：Applied and environmental microbiology

authors： Kästner M,Breuer-Jammali M,Mahro B

更新日期：1998-01-01 00:00:00

abstract：:FK506 is an important 23-member polyketide macrolide with immunosuppressant activity. Its entire biosynthetic gene cluster was previously cloned from Streptomyces sp. strain KCTC 11604BP, and sequence analysis identified three putative regulatory genes, tcs2, tcs7, and fkbN, which encode proteins with high similarity ...

journal_title：Applied and environmental microbiology

authors： Mo S,Yoo YJ,Ban YH,Lee SK,Kim E,Suh JW,Yoon YJ

更新日期：2012-04-01 00:00:00

abstract：:Campy-Cefex, a modification of Campy-Cefex, modified charcoal cefoperazone deoxycholate (mCCDA), Karmali, CAMPY, and Campy-Line agars were evaluated for their efficiency to isolate and enumerate Campylobacter spp. from poultry carcass rinses. Campy-Cefex and its modification produced the best results but were statisti...

journal_title：Applied and environmental microbiology

authors： Oyarzabal OA,Macklin KS,Barbaree JM,Miller RS

更新日期：2005-06-01 00:00:00

abstract：:Anaerobic acetate degradation at 70 degrees C and at 55 degrees C (as a reference) was studied by running laboratory upflow anaerobic sludge blanket (UASB) reactors inoculated with mesophilic granular sludge. In UASB reactors fed with acetate-containing media (3 g of chemical oxygen demand [COD] per liter, correspondi...

journal_title：Applied and environmental microbiology

authors： Rintala J,Lepistö S,Ahring B

更新日期：1993-06-01 00:00:00

abstract：:Northeastern Atlantic minke whales (Balaenoptera acutorostrata) have a multichambered stomach system which includes a nonglandular forestomach resembling that of ruminants. Bacteria from the forestomachs of herring-eating whales were enumerated and isolated in an anaerobic rumen-like culture medium (M8W medium). The t...

journal_title：Applied and environmental microbiology

authors： Olsen MA,Aagnes TH,Mathiesen SD

更新日期：1994-12-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, P...

journal_title：Applied and environmental microbiology

authors： Abeijón Mukdsi MC,Falentin H,Maillard MB,Chuat V,Medina RB,Parayre S,Thierry A

更新日期：2014-01-01 00:00:00

abstract：:Broiler chickens from eight commercial farms in Southern Finland were analyzed for the structure of their gastrointestinal microbial community by a nonselective DNA-based method, percent G+C-based profiling. The bacteriological impact of the feed source and in-farm whole-wheat amendment of the diet was assessed by per...

journal_title：Applied and environmental microbiology

authors： Apajalahti JH,Kettunen A,Bedford MR,Holben WE

更新日期：2001-12-01 00:00:00

abstract：:Subsurface sediments were recovered from a 52-m-deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the ...

journal_title：Applied and environmental microbiology

authors： Lin X,Kennedy D,Peacock A,McKinley J,Resch CT,Fredrickson J,Konopka A

更新日期：2012-02-01 00:00:00

abstract：:Comparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence...

journal_title：Applied and environmental microbiology

authors： Tardy F,Mick V,Dordet-Frisoni E,Marenda MS,Sirand-Pugnet P,Blanchard A,Citti C

更新日期：2015-03-01 00:00:00

abstract：:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blen...

journal_title：Applied and environmental microbiology

authors： Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

更新日期：1990-06-01 00:00:00

abstract：:Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PC...

journal_title：Applied and environmental microbiology

authors： Weyman PD,Pratte B,Thiel T

更新日期：2008-04-01 00:00:00

abstract：:Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and ...

journal_title：Applied and environmental microbiology

authors： Robinson IM,Whipp SC,Bucklin JA,Allison MJ

更新日期：1984-11-01 00:00:00

abstract：:Growth inhibition by isoleucine hydroxamate in Serratia marcescens was significantly enhanced by adding valine plus leucine and by using glycerol as the carbon source. Isoleucine hydroxamate-resistant mutants were isolated under conditions in which growth inhibition was enhanced. One of the mutants, strain GIHVLr2179,...

journal_title：Applied and environmental microbiology

authors： Kisumi M,Komatsubara S,Chibata I

更新日期：1977-12-01 00:00:00

abstract：:Three Methocel methylcellulose ethers of 1.9 degree of substitution with [14C]methyl labels were shown to be biodegradable using batch-type activated sludge tests. The maximum rate for conversion to 14CO2, attained after 1 week, was only 0.62 mg of methylcellulose/g of mixed liquor volatile solids per day. In 20 days,...

journal_title：Applied and environmental microbiology

authors： Blanchard FA,Takahashi IT,Alexander HC

更新日期：1976-10-01 00:00:00

abstract：:Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccase...

journal_title：Applied and environmental microbiology

authors： Camarero S,Ibarra D,Martínez MJ,Martínez AT

更新日期：2005-04-01 00:00:00

abstract：:This study investigated the differences in microbial community abundance, composition and diversity throughout the depth profiles in soils collected from corn and soybean fields in lowa, USA using 16S rRNA amplicon sequencing. The results revealed decreased richness and diversity in microbial communities at increasing...

journal_title：Applied and environmental microbiology

authors： Hao J,Chai YN,Lopes LD,Ordóñez RA,Wright EE,Archontoulis S,Schachtman DP

更新日期：2020-12-11 00:00:00

abstract：:The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutar...

journal_title：Applied and environmental microbiology

authors： Djurdjevic I,Zelder O,Buckel W

更新日期：2011-01-01 00:00:00

abstract：:Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium ...

journal_title：Applied and environmental microbiology

authors： Muñoz G,Sotomayor P

更新日期：1990-07-01 00:00:00

abstract：:The effects in the chicken embryo assay of four Alternaria metabolites (alternariol [AOH], alternariol methyl ether [AME], altenuene [ALT], and tenuazonic acid [TA]) were investigated. Administered to 7-day-old chicken embryos by yolk sac injection, AOH, AME, and ALT caused no mortality or teratogenic effect at doses ...

journal_title：Applied and environmental microbiology

authors： Griffin GF,Chu FS

更新日期：1983-12-01 00:00:00

abstract：:In a field experiment, Pseudomonas cepacia J82rif and J51rif increased sunflower emergence in the presence of the fungus Sclerotinia sclerotiorum. Pyrrolnitrin, aminopyrrolnitrin, and monochloroaminopyrrolnitrin were isolated from J82 and identified by using thin-layer chromatography, high-performance liquid chromatog...

journal_title：Applied and environmental microbiology

authors： McLoughlin TJ,Quinn JP,Bettermann A,Bookland R

更新日期：1992-05-01 00:00:00

abstract：:Hybrubins are "unnatural" alkaloids with the same 4'-methoxy-2,2'-bipyrrole-5'-methine moiety found in prodiginines and a different ring derived from tetramic acids. Here, we demonstrated that RedH, a homologue of prodigiosin synthetase PigC, was responsible for the biosynthesis of hybrubins A and B in Streptomyces li...

journal_title：Applied and environmental microbiology

authors： Long Q,Jeffries DE,Lin S,Chen X,He W,Wang Y,Deng Z,Lindsley CW,Tao M

更新日期：2020-01-07 00:00:00