Abstract:
:Growth of Clostridium thermocellum in batch cultures was studied over a broad range of cellobiose concentrations. Cultures displayed important differences in their substrate metabolism as determined by the end product yields. Bacterial growth was severely limited when the initial cellobiose concentration was 0.2 (wt/vol), was maximal at substrate concentrations between 0.5 and 2.0%, and did not occur at 5.0% cellobiose. Ethanol accumulated maximally (38.3 mumol/10 cells) in cultures with an initial cellobiose concentration of 0.8%, whereas cultures in 2.0% cellobiose accumulated only 17.3 mumol, and substrate-limited cultures (0.2% cellobiose) accumulated little, if any, ethanol beyond that initially detected (8.3 mumol/10 cells). In a medium with 0.8% cellobiose, ethanol was produced at a constant rate of approximately 1.1 mumol/10 cells per h from late-logarithmic phase (16 h) of growth well into stationary phase (44 h). When ethanol was added exogenously at levels more than twice the maximum produced by the cultures themselves (0.5% [vol/vol]), neither the extent of growth (maximum Klett units, 150) nor the amounts of ethanol produced ( approximately 0.17%) by the culture was affected. The ratio of ethanol to acetate was highest (2.8) when cells were grown in 0.8% cellobiose and lowest (1.2) when cells were grown in 0.2% cellobiose.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Brener D,Johnson BFdoi
10.1128/AEM.47.5.1126-1129.1984keywords:
subject
Has Abstractpub_date
1984-05-01 00:00:00pages
1126-9issue
5eissn
0099-2240issn
1098-5336journal_volume
47pub_type
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