Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains.

Abstract:

:Listeria monocytogenes is a food pathogen that can attach on most of the surfaces encountered in the food industry. Biofilms are three-dimensional microbial structures that facilitate the persistence of pathogens on surfaces, their resistance toward antimicrobials, and the final contamination of processed goods. So far, little is known about the structural dynamics of L. monocytogenes biofilm formation and its regulation. The aims of this study were, by combining genetics and time-lapse laser-scanning confocal microscopy (LSCM), (i) to characterize the structural dynamics of L. monocytogenes EGD-e sessile growth in two nutritional environments (with or without a nutrient flow), and (ii) to evaluate the possible role of the L. monocytogenes agr system during biofilm formation by tracking the spatiotemporal fluorescence expression of a green fluorescent protein (GFP) reporter system. In the absence of nutrient flow (static conditions), unstructured biofilms composed of a few layers of cells that covered the substratum were observed. In contrast, when grown under dynamic conditions, L. monocytogenes EGD-e biofilms were highly organized. Indeed, ball-shaped microcolonies were surrounded by a network of knitted chains. The spatiotemporal tracking of fluorescence emitted by the GFP reporter system revealed that agr expression was barely detectable under static conditions, but it progressively increased during 40 h under dynamic conditions. Moreover, spatial analysis revealed that agr was expressed preferentially in cells located outside the microcolonies. Finally, the in-frame deletion of agrA, which encodes a transcriptional regulator, resulted in a decrease in initial adherence without affecting the subsequent biofilm development.

journal_name

Appl Environ Microbiol

authors

Rieu A,Briandet R,Habimana O,Garmyn D,Guzzo J,Piveteau P

doi

10.1128/AEM.00255-08

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

4491-7

issue

14

eissn

0099-2240

issn

1098-5336

pii

AEM.00255-08

journal_volume

74

pub_type

杂志文章
  • Transformation of mercuric chloride and methylmercury by the rumen microflora.

    abstract::The microflora in strained rumen fluid did not methylate or volatilize 203Hg2+ at detectable rates. However, there was an exponential decay in the concentration of added CH3Hg+, which was attributed to demethylation. The major product of demethylation was metallic mercury (Hg0), and it was released as a volatile produ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.4.626-636.1979

    authors: Kozak S,Forsberg CW

    更新日期:1979-10-01 00:00:00

  • Acinetobacter insertion sequence ISAba11 belongs to a novel family that encodes transposases with a signature HHEK motif.

    abstract::Experimental and in silico PCR analysis targeting ISAba11 and TnAbaR islands in 196 epidemiologically unrelated Acinetobacter strains representative of ≥19 species were performed. The first two Acinetobacter baumannii ISAba11 elements identified had been found to map to the same site on TnAbaR transposons. However, no...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05663-11

    authors: Rieck B,Tourigny DS,Crosatti M,Schmid R,Kochar M,Harrison EM,Ou HY,Turton JF,Rajakumar K

    更新日期:2012-01-01 00:00:00

  • Enumeration, isolation, and characterization of beggiatoa from freshwater sediments.

    abstract::An accurate most-probable-number enumeration method was developed for counting the number of Beggiatoa trichomes from various freshwater sediments. The medium consisted of extracted hay, diluted soil extract, 0.05% acetate, and 15 to 35 U of catalase per ml. The same enrichment medium, but without the acetate, was the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.5.755-770.1978

    authors: Strohl WR,Larkin JM

    更新日期:1978-11-01 00:00:00

  • Restriction fragment length polymorphism evidence for genetic homology within a pathovar of Pseudomonas syringae.

    abstract::Pseudomonas syringae pv. phaseolicola NPS3121 hrp sequences were used as hybridization probes in a restriction fragment length polymorphism (RFLP) analysis of 24 P. syringae pv. tabaci strains as a means to evaluate the genetic and taxonomic relationship of pathovars of P. syringae. Southern blot analyses of genomic r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.4.1093-1100.1994

    authors: Scholz BK,Jakobek JL,Lindgren PB

    更新日期:1994-04-01 00:00:00

  • Autoinducer-2-producing protein LuxS, a novel salt- and chloride-induced protein in the moderately halophilic bacterium Halobacillus halophilus.

    abstract::The moderately halophilic bacterium Halobacillus halophilus carries a homologue of LuxS, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxS of H. halophilus is part of an operon that encodes an S-adenosylmethionine-dependent...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01625-06

    authors: Sewald X,Saum SH,Palm P,Pfeiffer F,Oesterhelt D,Müller V

    更新日期:2007-01-01 00:00:00

  • Cellulosilyticum ruminicola, a newly described rumen bacterium that possesses redundant fibrolytic-protein-encoding genes and degrades lignocellulose with multiple carbohydrate- borne fibrolytic enzymes.

    abstract::Cellulosilyticum ruminicola H1 is a newly described bacterium isolated from yak (Bos grunniens) rumen and is characterized by its ability to grow on a variety of hemicelluloses and degrade cellulosic materials. In this study, we performed the whole-genome sequencing of C. ruminicola H1 and observed a comprehensive set...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03124-09

    authors: Cai S,Li J,Hu FZ,Zhang K,Luo Y,Janto B,Boissy R,Ehrlich G,Dong X

    更新日期:2010-06-01 00:00:00

  • Morphological and compositional changes in a planktonic bacterial community in response to enhanced protozoan grazing.

    abstract::We analyzed changes in bacterioplankton morphology and composition during enhanced protozoan grazing by image analysis and fluorescent in situ hybridization with group-specific rRNA-targeted oligonucleotide probes. Enclosure experiments were conducted in a small, fishless freshwater pond which was dominated by the cla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.3.1241-1250.1999

    authors: Jürgens K,Pernthaler J,Schalla S,Amann R

    更新日期:1999-03-01 00:00:00

  • Degradation of 4-Chlorobenzoic Acid by Arthrobacter sp.

    abstract::A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.5.1020-1025.1984

    authors: Marks TS,Smith AR,Quirk AV

    更新日期:1984-11-01 00:00:00

  • The weak acid preservative sorbic acid inhibits conidial germination and mycelial growth of Aspergillus niger through intracellular acidification.

    abstract::The growth of the filamentous fungus Aspergillus niger, a common food spoilage organism, is inhibited by the weak acid preservative sorbic acid (trans-trans-2,4-hexadienoic acid). Conidia inoculated at 10(5)/ml of medium showed a sorbic acid MIC of 4.5 mM at pH 4.0, whereas the MIC for the amount of mycelia at 24 h de...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.6.3506-3511.2004

    authors: Plumridge A,Hesse SJ,Watson AJ,Lowe KC,Stratford M,Archer DB

    更新日期:2004-06-01 00:00:00

  • Engineering of bacterial methyl ketone synthesis for biofuels.

    abstract::We have engineered Escherichia coli to overproduce saturated and monounsaturated aliphatic methyl ketones in the C₁₁ to C₁₅ (diesel) range; this group of methyl ketones includes 2-undecanone and 2-tridecanone, which are of importance to the flavor and fragrance industry and also have favorable cetane numbers (as we re...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06785-11

    authors: Goh EB,Baidoo EE,Keasling JD,Beller HR

    更新日期:2012-01-01 00:00:00

  • Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability.

    abstract::Mutacin 1140 belongs to the epidermin family of type AI lantibiotics. This family has a broad spectrum of activity against Gram-positive bacteria. The binding of mutacin 1140 to lipid II leads to the inhibition of cell wall synthesis. Pharmacokinetic experiments with type AI lantibiotics are generally discouraging for...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00830-18

    authors: Geng M,Smith L

    更新日期:2018-07-17 00:00:00

  • Analysis of Campylobacter jejuni Subtype Distribution in the Chicken Broiler Production Continuum: a Longitudinal Examination To Identify Primary Contamination Points.

    abstract::Significant knowledge gaps exist in our understanding of Campylobacter jejuni contamination of the poultry production continuum. Microbiological surveillance and genotypic characterization were undertaken on C. jejuni isolates longitudinally recovered from three poultry farms (weekly samples), the abattoir at which bi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02001-20

    authors: Inglis GD,Ramezani N,Taboada EN,Boras VF,Uwiera RRE

    更新日期:2021-01-15 00:00:00

  • High prevalence of Enterocytozoon bieneusi in asymptomatic pigs and assessment of zoonotic risk at the genotype level.

    abstract::Enterocytozoon bieneusi is an emerging and clinically significant enteric parasite infecting humans and animals and can cause life-threatening diarrhea in immunocompromised people. Pigs are considered to be one of the main reservoir hosts of E. bieneusi based on their high prevalence rates and zoonotic genotypes in pi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00807-14

    authors: Zhao W,Zhang W,Yang F,Cao J,Liu H,Yang D,Shen Y,Liu A

    更新日期:2014-06-01 00:00:00

  • RNA arbitrarily primed PCR and fourier transform infrared spectroscopy reveal plasticity in the acid tolerance response of Streptococcus macedonicus.

    abstract::We have previously reported that an acid tolerance response (ATR) can be induced in Streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic pH, or acid habituation, as well as at stationary phase. Here, we compared the transcriptional profiles of these epigenetic phenotyp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00315-08

    authors: Papadimitriou K,Boutou E,Zoumpopoulou G,Tarantilis PA,Polissiou M,Vorgias CE,Tsakalidou E

    更新日期:2008-10-01 00:00:00

  • Biochemical and Molecular Characterization of Obesumbacterium proteus, a Common Contaminant of Brewing Yeasts.

    abstract::We have evaluated the effectiveness of API 20E, Biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (ERIC)-PCR to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant Obesumbacterium proteus. Of the five typing techniques, Biolog tes...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.5.1635-1640.1994

    authors: Prest AG,Hammond JR,Stewart GS

    更新日期:1994-05-01 00:00:00

  • Distribution of methanogenic and sulfate-reducing bacteria in near-shore marine sediments.

    abstract::The distribution of methanogenic and sulfate-reducing bacteria was examined in sediments from three sites off the coast of eastern Connecticut and five sites in Long Island Sound. Both bacterial groups were detected at all sites. Three distributional patterns were observed: (i) four sites exhibited methanogenic and su...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.2.447-453.1982

    authors: Hines ME,Buck JD

    更新日期:1982-02-01 00:00:00

  • Geographical Differentiation of the Population of Xanthomonas axonopodis pv. manihotis in Colombia.

    abstract::[This corrects the article on p. 4430 in vol. 63.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:

    authors: Restrepo S,Verdier V

    更新日期:1998-03-01 00:00:00

  • Salmonella and Escherichia coli O157:H7 survival in soil and translocation into leeks (Allium porrum) as influenced by an arbuscular mycorrhizal fungus (Glomus intraradices).

    abstract::A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on Salmonella and enterohemorrhagic Escherichia coli O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc-] AM fungi) were inoculated with composite suspensions o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02855-12

    authors: Gurtler JB,Douds DD Jr,Dirks BP,Quinlan JJ,Nicholson AM,Phillips JG,Niemira BA

    更新日期:2013-03-01 00:00:00

  • Biosurfactant production by a soil pseudomonas strain growing on polycyclic aromatic hydrocarbons.

    abstract::The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.6.1908-1912.1996

    authors: Deziel E,Paquette G,Villemur R,Lepine F,Bisaillon J

    更新日期:1996-06-01 00:00:00

  • DbdR, a New Member of the LysR Family of Transcriptional Regulators, Coordinately Controls Four Promoters in the Thauera aromatica AR-1 3,5-Dihydroxybenzoate Anaerobic Degradation Pathway.

    abstract::The facultative anaerobe Thauera aromatica strain AR-1 uses 3,5-dihydroxybenzoate (3,5-DHB) as a sole carbon and energy source under anoxic conditions using an unusual oxidative strategy to overcome aromatic ring stability. A 25-kb gene cluster organized in four main operons encodes the anaerobic degradation pathway f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02295-18

    authors: Pacheco-Sánchez D,Molina-Fuentes Á,Marín P,Díaz-Romero A,Marqués S

    更新日期:2019-01-09 00:00:00

  • Antifungal activity of Wickerhamomyces anomalus and Lactobacillus plantarum during sourdough fermentation: identification of novel compounds and long-term effect during storage of wheat bread.

    abstract::This study aimed at investigating the antifungal activity of Wickerhamomyces anomalus and sourdough lactic acid bacteria to extend the shelf life of wheat flour bread. The antifungal activity was assayed by agar diffusion, growth rate inhibition, and conidial germination assays, using Penicillium roqueforti DPPMAF1 as...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02669-10

    authors: Coda R,Cassone A,Rizzello CG,Nionelli L,Cardinali G,Gobbetti M

    更新日期:2011-05-01 00:00:00

  • Transformation of isopropylamine to L-alaninol by Pseudomonas sp. strain KIE171 involves N-glutamylated intermediates.

    abstract::Pseudomonas sp. strain KIE171 was able to grow with isopropylamine or L-alaninol [S-(+)-2-amino-1-propanol] as the sole carbon source, but not with D-alaninol. To investigate the hypothesis that L-alaninol is an intermediate in the degradation of isopropylamine, two mini-Tn5 mutants unable to utilize both isopropylami...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.5.2368-2375.2002

    authors: de Azevedo Wäsch SI,van der Ploeg JR,Maire T,Lebreton A,Kiener A,Leisinger T

    更新日期:2002-05-01 00:00:00

  • Delineation of key amino acid side chains and peptide domains for antimicrobial properties of divercin V41, a pediocin-like bacteriocin secreted by Carnobacterium divergens V41.

    abstract::Divercin V41 (DV41) is a class IIa bacteriocin produced by Carnobacterium divergens V41. This antilisterial peptide is homologous to pediocin PA-1 and contains two disulfide bonds. To establish the structure-activity relationships of this specific family of bacteriocin, chemical modifications and enzymatic hydrolysis ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.7.2895-2900.1999

    authors: Bhugaloo-Vial P,Douliez JP,Moll D,Dousset X,Boyaval P,Marion D

    更新日期:1999-07-01 00:00:00

  • Divergent Nrf Family Proteins and MtrCAB Homologs Facilitate Extracellular Electron Transfer in Aeromonas hydrophila.

    abstract::Extracellular electron transfer (EET) is a strategy for respiration in which electrons generated from metabolism are moved outside the cell to a terminal electron acceptor, such as iron or manganese oxide. EET has primarily been studied in two model systems, Shewanella oneidensis and Geobacter sulfurreducens Metal red...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02134-18

    authors: Conley BE,Intile PJ,Bond DR,Gralnick JA

    更新日期:2018-11-15 00:00:00

  • Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium.

    abstract::Strain DCB-1 is an obligately anaerobic bacterium which carries out the reductive dehalogenation of halobenzoates and was previously known to grow only on pyruvate plus 20% ruminal fluid. When various electron acceptors were supplied, thiosulfate and sulfite were found to stimulate growth. Sulfide was produced from th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.2938-2943.1988

    authors: Stevens TO,Linkfield TG,Tiedje JM

    更新日期:1988-12-01 00:00:00

  • Absence of livestock-associated methicillin-resistant staphylococcus aureus clonal complex CC398 as a nasal colonizer of pigs raised in an alternative system.

    abstract::Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 isolated from pigs raised in conventional farms was previously reported. Here we report a study on 25 farms adhering to an alternative system. LA-MRSA ST398 was not detected in nasal swabs from 178 pigs or from 89 humans working and livin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07260-11

    authors: Cuny C,Friedrich AW,Witte W

    更新日期:2012-02-01 00:00:00

  • Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus.

    abstract::Two-component regulatory systems are one primary mechanism for environmental sensing and signal transduction. Annotation of the complete genome sequence of the probiotic bacterium Lactobacillus acidophilus NCFM revealed nine two-component regulatory systems. In this study, the histidine protein kinase of a two-compone...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.10.5794-5804.2005

    authors: Azcarate-Peril MA,McAuliffe O,Altermann E,Lick S,Russell WM,Klaenhammer TR

    更新日期:2005-10-01 00:00:00

  • Climate and tick seasonality are predictors of Borrelia burgdorferi genotype distribution.

    abstract::The blacklegged tick, Ixodes scapularis, is of significant public health importance as a vector of Borrelia burgdorferi, the agent of Lyme borreliosis. The timing of seasonal activity of each immature I. scapularis life stage relative to the next is critical for the maintenance of B. burgdorferi because larvae must fe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02633-08

    authors: Gatewood AG,Liebman KA,Vourc'h G,Bunikis J,Hamer SA,Cortinas R,Melton F,Cislo P,Kitron U,Tsao J,Barbour AG,Fish D,Diuk-Wasser MA

    更新日期:2009-04-01 00:00:00

  • Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene.

    abstract::Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Nonino...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8500-8505.2005

    authors: Taghavi S,Barac T,Greenberg B,Borremans B,Vangronsveld J,van der Lelie D

    更新日期:2005-12-01 00:00:00

  • Improved enumeration of lactic Acid streptococci on elliker agar containing phosphate.

    abstract::Enumeration of lactic acid streptococci from dairy starter cultures on Elliker agar was improved by the addition of 0.4% (wt/vol) diammonium phosphate. Agar plate counts were up to 7.75 times greater due to improved buffering by the phosphate salt. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.1.173-174.1979

    authors: Barach JT

    更新日期:1979-07-01 00:00:00