Involvement of cell surface structures in size-independent grazing resistance of freshwater Actinobacteria.

abstract：:The influence of glucose concentration on Cd, Cu, Hg, and Zn toxicity to a Klebsiella sp. was studied by following the degradation of C-labeled glucose at pH 6.0. Uptake of C into the cells was also determined. The carbon concentrations ranged from 0.01 to 40 mg liter, which are equivalent to soluble C concentrations ...

journal_title：Applied and environmental microbiology

authors： Brynhildsen L,Lundgren BV,Allard B,Rosswall T

更新日期：1988-07-01 00:00:00

abstract：:We developed an improved method for cultivation-independent sorting of bacterial cells. The technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. Due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the c...

journal_title：Applied and environmental microbiology

authors： Zwirglmaier K,Ludwig W,Schleifer KH

更新日期：2004-01-01 00:00:00

abstract：:The ability of Escherichia coli MV1184 to accumulate inorganic phosphate (Pi) was enhanced by manipulating the genes involved in the transport and metabolism of Pi. The high-level Pi accumulation was achieved by modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kina...

journal_title：Applied and environmental microbiology

authors： Kato J,Yamada K,Muramatsu A,Hardoyo,Ohtake H

更新日期：1993-11-01 00:00:00

abstract：:Seventeen Bradyrhizobium sp. strains and one Azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16S rRNA gene (rDNA), (ii) randomly amplified DNA polymorphisms (RAPD) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv)...

journal_title：Applied and environmental microbiology

authors： Van Rossum D,Schuurmans FP,Gillis M,Muyotcha A,Van Verseveld HW,Stouthamer AH,Boogerd FC

更新日期：1995-04-01 00:00:00

abstract：:To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which ...

journal_title：Applied and environmental microbiology

authors： Wu H,Gao K,Villafañe VE,Watanabe T,Helbling EW

更新日期：2005-09-01 00:00:00

abstract：:Lysostaphin represents a promising therapeutic agent for the treatment of staphylococcal infections, in particular those of methicillin-resistant Staphylococcus aureus (MRSA). However, conventional expression systems for the enzyme suffer from various limitations, and there remains a need for an efficient and cost-eff...

journal_title：Applied and environmental microbiology

authors： Zhao H,Blazanovic K,Choi Y,Bailey-Kellogg C,Griswold KE

更新日期：2014-05-01 00:00:00

abstract：:The contributions of various components of soil microflora and microfauna to rhizosphere phosphatase activity were determined with hydroponic cultures. Three treatments were employed: (i) plants alone (Bouteloua gracilis (H.B.K.) Lag. ex Steud.) (ii) plants plus bacteria (Pseudomonas sp.), and (iii) plants plus bacter...

journal_title：Applied and environmental microbiology

authors： Gould WD,Coleman DC,Rubink AJ

更新日期：1979-05-01 00:00:00

abstract：:Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

journal_title：Applied and environmental microbiology

authors： Hamdan H,Weller DM,Thomashow LS

更新日期：1991-11-01 00:00:00

abstract：:Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner and outer membranes. In the present study, we developed a method by which the porin AlgE was detect...

journal_title：Applied and environmental microbiology

authors： Maleki S,Almaas E,Zotchev S,Valla S,Ertesvåg H

更新日期：2015-12-11 00:00:00

abstract：:The D-values of Bacillus pumilus spores on various devices ranged from 0.14 to 0.23 Mrads. The majority of devices displayed D-values equal to or less than the value obtained on filter paper. Increased resistivity was also encountered. ...

journal_title：Applied and environmental microbiology

authors： Prince HN

更新日期：1978-08-01 00:00:00

abstract：:Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypotheti...

journal_title：Applied and environmental microbiology

authors： Simmons K,Rempel H,Block G,Forgetta V,Vaillancourt R Jr,Malouin F,Topp E,Delaquis P,Diarra MS

更新日期：2014-03-01 00:00:00

abstract：:Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary r...

journal_title：Applied and environmental microbiology

authors： Ponnusamy L,Gonzalez A,Van Treuren W,Weiss S,Parobek CM,Juliano JJ,Knight R,Roe RM,Apperson CS,Meshnick SR

更新日期：2014-01-01 00:00:00

abstract：:The genus Sepiola (Cephalopoda: Sepiolidae) contains 10 known species that occur in the Mediterranean Sea today. All Sepiola species have a light organ that contains at least one of two species of luminous bacteria, Vibrio fischeri and Vibrio logei. The two Vibrio species coexist in at least four Sepiola species (S. a...

journal_title：Applied and environmental microbiology

authors： Nishiguchi MK

更新日期：2000-08-01 00:00:00

abstract：:Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

更新日期：2002-02-01 00:00:00

abstract：:The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash s...

journal_title：Applied and environmental microbiology

authors： Shen C,Luo Y,Nou X,Bauchan G,Zhou B,Wang Q,Millner P

更新日期：2012-10-01 00:00:00

abstract：:The lysogenic bacteriophage APSE infects "Candidatus Hamiltonella defensa," a facultative endosymbiont of aphids and other sap-feeding insects. This endosymbiont has established a beneficial association with aphids, increasing survivorship following attack by parasitoid wasps. Although APSE and "Ca. Hamiltonella defen...

journal_title：Applied and environmental microbiology

authors： Degnan PH,Moran NA

更新日期：2008-11-01 00:00:00

abstract：:Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of Bacillus cereus ATCC 14579 exposed to four different disinfectants (b...

journal_title：Applied and environmental microbiology

authors： Ceragioli M,Mols M,Moezelaar R,Ghelardi E,Senesi S,Abee T

更新日期：2010-05-01 00:00:00

abstract：:Deep-subsurface samples obtained by deep drilling are likely to be contaminated with mesophilic microorganisms in the drilling fluid, and this could affect determination of the community structure of the geothermal microflora using 16S rRNA gene clone library analysis. To eliminate possible contamination by PCR-amplif...

journal_title：Applied and environmental microbiology

authors： Kimura H,Sugihara M,Kato K,Hanada S

更新日期：2006-01-01 00:00:00

abstract：:By targeted deletion of the polyglutamate operon (pga) in Bacillus licheniformis F11, a derivative form, F11.1 (Δpga), was obtained that, along with lacking polyglutamate (PGA) formation, displayed enhanced proteolytic activities. The phenotypic properties were maintained in a strain in which the chiBA operon was addi...

journal_title：Applied and environmental microbiology

authors： Hoffmann K,Daum G,Köster M,Kulicke WM,Meyer-Rammes H,Bisping B,Meinhardt F

更新日期：2010-12-01 00:00:00

abstract：:An aseptic vacuum extraction technique was used to obtain xylem fluid from the roots of rough lemon (Citrus jambhiri Lush.) rootstock of Florida citrus trees. Bacteria were consistently isolated from vascular fluid of both healthy and young tree decline-affected trees. Thirteen genera of bacteria were found, the most ...

journal_title：Applied and environmental microbiology

authors： Gardner JM,Feldman AW,Zablotowicz RM

更新日期：1982-06-01 00:00:00

abstract：:Recently, the lignin-degrading basidiomycete Pleurotus ostreatus has become a widely used model organism for fungal genomic and transcriptomic analyses. The increasing interest in this species has led to an increasing number of studies analyzing the transcriptional regulation of multigene families that encode extracel...

journal_title：Applied and environmental microbiology

authors： Castanera R,López-Varas L,Pisabarro AG,Ramírez L

更新日期：2015-06-15 00:00:00

abstract：:"Caldocellum saccharolyticum" is an obligatory anaerobic thermophilic bacterium. A gene from this organism, designated celB, has been cloned in Escherichia coli as part of a bacteriophage lambda gene library. This gene produces a thermostable cellulase that shows both endoglucanase and exoglucanase activities on test ...

journal_title：Applied and environmental microbiology

authors： Saul DJ,Williams LC,Grayling RA,Chamley LW,Love DR,Bergquist PL

更新日期：1990-10-01 00:00:00

abstract：:Strain UHM-5, a pSym Exo derivative of the aluminum-tolerant Rhizobium leguminosarum bv. phaseoli strain CIAT899, was equally tolerant of aluminum (Al) as the parental culture. Dialyzed culture supernatants of the wild-type cells grown in YEM broth (10 cells ml) contained 185 mug of glucose equivalents ml whereas UHM-...

journal_title：Applied and environmental microbiology

authors： Kingsley MT,Bohlool BB

更新日期：1992-04-01 00:00:00

abstract：:Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable le...

journal_title：Applied and environmental microbiology

authors： Santos F,Teusink B,Molenaar D,van Heck M,Wels M,Sieuwerts S,de Vos WM,Hugenholtz J

更新日期：2009-06-01 00:00:00

abstract：:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential...

journal_title：Applied and environmental microbiology

authors： Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

更新日期：2008-07-01 00:00:00

abstract：:The composition of the active microbial (bacterial and fungal) soil community in an arable wheat field subjected to different management practices was examined at five times during a 1-year period. Field sections were fertilized either at good agricultural practice (GAP) levels or at reduced levels (0.5x GAP) and were...

journal_title：Applied and environmental microbiology

authors： Girvan MS,Bullimore J,Ball AS,Pretty JN,Osborn AM

更新日期：2004-05-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:Microcystis aeruginosa is a common cyanobacterium in water blooms that appear widely in nutrient-rich, fresh, and brackish waters, and its toxic blooms cause the death of domestic animals. The administration of a crude toxic cell extract of M. aeruginosa K-139 to mice can produce tumor necrosis factor (TNF) and prompt...

journal_title：Applied and environmental microbiology

authors： Nakano Y,Shirai M,Mori N,Nakano M

更新日期：1991-01-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:Salmonella typhimurium accumulates glutamate in response to osmotic stress. Cells in aerobic exponential growth have an intracellular pool of approximately 125 nmol of glutamate mg of protein-1. When cells were grown in minimal medium with 500 mM NaCl, KCl, or sucrose, 290 to 430 nmol of glutamate was found to accumul...

journal_title：Applied and environmental microbiology

authors： Botsford JL,Alvarez M,Hernandez R,Nichols R

更新日期：1994-07-01 00:00:00