Glycoprotein hypersecretion alters the cell wall in Trichoderma reesei strains expressing the Saccharomyces cerevisiae dolichylphosphate mannose synthase gene.

abstract：:Because of high diurnal water quality fluctuations in raw municipal wastewater, the use of proportional autosampling over a period of 24 h at municipal wastewater treatment plants (WWTPs) to evaluate carbon, nitrogen, and phosphorus removal has become a standard in many countries. Microbial removal or load estimation ...

journal_title：Applied and environmental microbiology

authors： Mayer RE,Vierheilig J,Egle L,Reischer GH,Saracevic E,Mach RL,Kirschner AK,Zessner M,Sommer R,Farnleitner AH

更新日期：2015-08-01 00:00:00

abstract：:At certain stages in its growth cycle, Methanosarcina mazei produces an enzyme (disaggregatase) that causes aggregates to separate into single cells. M. mazei S-6 and LYC both produce this enzymatic activity, although the specificities of activities differ. The disaggregatase of M. mazei S-6 had little effect on strai...

journal_title：Applied and environmental microbiology

authors： Xun LY,Mah RA,Boone DR

更新日期：1990-12-01 00:00:00

abstract：:Elevated winter losses of managed honeybee colonies are a major concern, but the underlying mechanisms remain controversial. Among the suspects are the parasitic mite Varroa destructor, the microsporidian Nosema ceranae, and associated viruses. Here we hypothesize that pathogens reduce the life expectancy of winter be...

journal_title：Applied and environmental microbiology

authors： Dainat B,Evans JD,Chen YP,Gauthier L,Neumann P

更新日期：2012-02-01 00:00:00

abstract：:Here, we report a novel method to produce microencapsulated enzymes using Saccharomyces cerevisiae spores. In sporulating cells, soluble secreted proteins are transported to the spore wall. Previous work has shown that the spore wall is capable of retaining soluble proteins because its outer layers work as a diffusion...

journal_title：Applied and environmental microbiology

authors： Shi L,Li Z,Tachikawa H,Gao XD,Nakanishi H

更新日期：2014-08-01 00:00:00

abstract：:After the first isolation of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in 2003, this MRSA variant quickly became the predominant MRSA obtained from humans as part of the Dutch national MRSA surveillance. Previous studies have suggested that human-to-human transmission of LA-MRSA, compa...

journal_title：Applied and environmental microbiology

authors： Bosch T,Verkade E,van Luit M,Landman F,Kluytmans J,Schouls LM

更新日期：2015-01-01 00:00:00

abstract：:Population sizes of algae, bacteria, heterotrophic flagellates, and viruses were observed through the 1989 spring diatom bloom in Raunefjorden in western Norway. The culmination of the diatom bloom was followed by a peak in the concentration of bacteria and an increase in the concentration of heterotrophic flagellates...

journal_title：Applied and environmental microbiology

authors： Bratbak G,Heldal M,Norland S,Thingstad TF

更新日期：1990-05-01 00:00:00

abstract：:The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter up...

journal_title：Applied and environmental microbiology

authors： Soltes-Rak E,Kushner DJ,Williams DD,Coleman JR

更新日期：1993-08-01 00:00:00

abstract：:The gene encoding a novel penicillin G acylase (PGA), designated pgaW, was cloned from Achromobacter xylosoxidans and overexpressed in Escherichia coli. The pgaW gene contains an open reading frame of 2586 nucleotides. The deduced protein sequence encoded by pgaW has about 50% amino acid identity to several well-chara...

journal_title：Applied and environmental microbiology

authors： Cai G,Zhu S,Yang S,Zhao G,Jiang W

更新日期：2004-05-01 00:00:00

abstract：:Of 199 fungal cultures isolated from some of the common cereals collected from different parts of Uttar Pradesh and Madhya Pradesh, 70 produced toxic metabolites. Of the 70 fungi isolated, 59 produced toxins which caused visible lesions in livers, kidneys, and spleens but did not cause mortality. Toxicity, graded in t...

journal_title：Applied and environmental microbiology

authors： Gupta J,Pathak B,Sethi N,Vora VC

更新日期：1981-03-01 00:00:00

abstract：:Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nucle...

journal_title：Applied and environmental microbiology

authors： Quirasco M,López-Munguía A,Remaud-Simeon M,Monsan P,Farrés A

更新日期：1999-12-01 00:00:00

abstract：:The crenarchaeon Sulfolobus acidocaldarius has been described to synthesize trehalose via the maltooligosyltrehalose synthase (TreY) and maltooligosyltrehalose trehalohydrolase (TreZ) pathway, and the trehalose glycosyltransferring synthase (TreT) pathway has been predicted. Deletion mutant analysis of strains with si...

journal_title：Applied and environmental microbiology

authors： Stracke C,Meyer BH,Hagemann A,Jo E,Lee A,Albers SV,Cha J,Bräsen C,Siebers B

更新日期：2020-11-24 00:00:00

abstract：:Azotobacter vinelandii selectively utilizes three types of nitrogenase (molybdenum, vanadium, and iron only) to fix N2, with their expression regulated by the presence or absence of different metal cofactors in its environment. Each alternative nitrogenase isoenzyme is predicted to have different electron flux require...

journal_title：Applied and environmental microbiology

authors： Natzke J,Noar J,Bruno-Bárcena JM

更新日期：2018-08-01 00:00:00

abstract：:The initial formation and spatiotemporal development of microbial biofilm layers on surfaces of new and clean reverse osmosis (RO) membranes and feed-side spacers were monitored in situ using flow cells placed in parallel with the RO system of a full-scale water treatment plant. The feed water of the RO system had bee...

journal_title：Applied and environmental microbiology

authors： Bereschenko LA,Stams AJ,Euverink GJ,van Loosdrecht MC

更新日期：2010-04-01 00:00:00

abstract：:A survey of Bacillus thuringiensis recovered from the environments of olive groves in Greece was carried out. Of 80 soil samples, 24 were found to contain B. thuringiensis with parasporal crystal inclusions; these were tested for toxicity against the olive fruit fly (Dacus oleae). Mortality levels of larvae caused by ...

journal_title：Applied and environmental microbiology

authors： Karamanlidou G,Lambropoulos AF,Koliais SI,Manousis T,Ellar D,Kastritsis C

更新日期：1991-08-01 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is critical for genome stability because of its roles in double-strand break repair. Ku and ligase D (LigD) are the crucial proteins in this process, and strains expressing Ku and LigD can cyclize linear DNA in vivo Here, we established a proof-of-concept single-homology-arm linear DNA...

journal_title：Applied and environmental microbiology

authors： Lu S,Nie Y,Wang M,Xu HX,Ma DL,Liang JL,Wu XL

更新日期：2018-09-17 00:00:00

abstract：:A bacterial biosensor for benzene, toluene, and similar compounds has been constructed, characterized, and field tested on contaminated water and soil. The biosensor is based on a plasmid incorporating the transcriptional activator xylR from the TOL plasmid of Pseudomonas putida mt-2. The XylR protein binds a subset o...

journal_title：Applied and environmental microbiology

authors： Willardson BM,Wilkins JF,Rand TA,Schupp JM,Hill KK,Keim P,Jackson PJ

更新日期：1998-03-01 00:00:00

abstract：:The extremely thermophilic anaerobic archaeon strain B1001 was isolated from a hot-spring environment in Japan. The cells were irregular cocci, 0.5 to 1.0 micrometers in diameter. The new isolate grew at temperatures between 60 and 95 degrees C (optimum, 85 degrees C), from pH 5.0 to 9.0 (optimum, pH 7.0), and from 1....

journal_title：Applied and environmental microbiology

authors： Tachibana Y,Kuramura A,Shirasaka N,Suzuki Y,Yamamoto T,Fujiwara S,Takagi M,Imanaka T

更新日期：1999-05-01 00:00:00

abstract：:Listeria monocytogenes is among the most important food-borne pathogens and is well adapted to persist in the environment. To gain insight into the genetic relatedness and potential virulence of L. monocytogenes strains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat a...

journal_title：Applied and environmental microbiology

authors： Balandyté L,Brodard I,Frey J,Oevermann A,Abril C

更新日期：2011-12-01 00:00:00

abstract：:Alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase (RdpA) and alpha-ketoglutarate-dependent (S)-dichlorprop dioxygenase (SdpA), which are involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH, were expressed and purified as His6-tagged fusion proteins from Escherichia...

journal_title：Applied and environmental microbiology

authors： Müller TA,Fleischmann T,van der Meer JR,Kohler HP

更新日期：2006-07-01 00:00:00

abstract：:We modeled mold growth on a solid culture medium at various temperatures and NaCl concentrations by using five common food spoilage molds (Penicillium roqueforti, Trichoderma harzianum, Paecilomyces variotii, Aspergillus niger, and Emericella nidulans). For the description of the growth rate (expressed as the increase...

journal_title：Applied and environmental microbiology

authors： Cuppers HG,Oomes S,Brul S

更新日期：1997-10-01 00:00:00

abstract：:Three strains of Streptococcus bovis, a homolactic bacterium capable of utilizing starch, were evaluated for growth kinetics and ability to decrease the pH of alfalfa silage. A selected strain was evaluated for its competitiveness as an inoculant with Enterococcus faecium, an organism used in inoculants, and for its a...

journal_title：Applied and environmental microbiology

authors： Jones BA,Muck RE,Ricke SC

更新日期：1991-10-01 00:00:00

abstract：:A "repair-detection" procedure consisting of pour plating of food samples with Trypticase soy agar, followed by 1-h repair incubation at room temperature and subsequent overlay with violet red bile agar, was found to be an effective method for the detection of injuried and uninjuried coliforms from dairy products. Thi...

journal_title：Applied and environmental microbiology

authors： Ray B,Speck ML

更新日期：1978-04-01 00:00:00

abstract：:The objective of the present study was to develop a mathematical model of pathogenic bacterial inactivation kinetics in a gastric environment in order to further understand a part of the infectious dose-response mechanism. The major bacterial pathogens Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella s...

journal_title：Applied and environmental microbiology

authors： Koseki S,Mizuno Y,Sotome I

更新日期：2011-02-01 00:00:00

abstract：:Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

journal_title：Applied and environmental microbiology

authors： Caskey WH,Taber WA

更新日期：1981-07-01 00:00:00

abstract：:We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, s...

journal_title：Applied and environmental microbiology

authors： Rollins DM,Coolbaugh JC,Walker RI,Weiss E

更新日期：1983-01-01 00:00:00

abstract：:Several members of the glycoside hydrolase 61 (GH61) family of proteins have recently been shown to dramatically increase the breakdown of lignocellulosic biomass by microbial hydrolytic cellulases. However, purified GH61 proteins have neither demonstrable direct hydrolase activity on various polysaccharide or lignaci...

journal_title：Applied and environmental microbiology

authors： Langston JA,Shaghasi T,Abbate E,Xu F,Vlasenko E,Sweeney MD

更新日期：2011-10-01 00:00:00

abstract：:The human pathogen Vibrio vulnificus is the leading cause of seafood-related deaths in the United States. Strains are genotyped on the basis of alleles that correlate with isolation source, with clinical (C)-genotype strains being more often implicated in disease and environmental (E)-genotype strains being more frequ...

journal_title：Applied and environmental microbiology

authors： Williams TC,Ayrapetyan M,Oliver JD

更新日期：2015-09-01 00:00:00

abstract：:The Amazon rainforest, the largest equatorial forest in the world, is being cleared for pasture and agricultural use at alarming rates. Tropical deforestation is known to cause alterations in microbial communities at taxonomic and phylogenetic levels, but it is unclear whether microbial functional groups are altered. ...

journal_title：Applied and environmental microbiology

authors： Mirza BS,Potisap C,Nüsslein K,Bohannan BJ,Rodrigues JL

更新日期：2014-01-01 00:00:00

abstract：:Different nutrient receptors varied in triggering germination of Bacillus subtilis spores with a pressure of 150 MPa, the GerA receptor being more responsive than the GerB receptor and even more responsive than the GerK receptor. This hierarchy in receptor responsiveness to pressure was the same as receptor responsive...

journal_title：Applied and environmental microbiology

authors： Black EP,Koziol-Dube K,Guan D,Wei J,Setlow B,Cortezzo DE,Hoover DG,Setlow P

更新日期：2005-10-01 00:00:00

abstract：:A methanogenic bioreactor that utilized wastepaper was developed and operated at 55 degrees C. Microbial community structure analysis showed the presence of a group of clostridia that specifically occurred during the period of high fermentation efficiency. To isolate the effective cellulose digester, the sludge that e...

journal_title：Applied and environmental microbiology

authors： Shiratori H,Ikeno H,Ayame S,Kataoka N,Miya A,Hosono K,Beppu T,Ueda K

更新日期：2006-05-01 00:00:00