Isolation and characterization of disaggregatase from Methanosarcina mazei LYC.

Abstract:

:At certain stages in its growth cycle, Methanosarcina mazei produces an enzyme (disaggregatase) that causes aggregates to separate into single cells. M. mazei S-6 and LYC both produce this enzymatic activity, although the specificities of activities differ. The disaggregatase of M. mazei S-6 had little effect on strain LYC cells, but the disaggregatase of M. mazei LYC disaggregated both strain LYC and strain S-6 cells. The disaggregatase of M. mazei LYC was purified by column chromatography, and it apparently consisted of two similar subunits with a combined molecular size of about 180,000 Da. Strain S-6 culture supernatants contained 14 U of activity per liter when activity was measured as uronic acids released from purified cell wall material. When the activity was quantified as the release of uronic acids from boiled M. mazei S-6 cells, the highest activity was found at pH 4.7 and at 35 degrees C.

journal_name

Appl Environ Microbiol

authors

Xun LY,Mah RA,Boone DR

doi

10.1128/AEM.56.12.3693-3698.1990

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

3693-8

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

56

pub_type

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