Liquid chromatographic procedure for fermentation product analysis in the identification of anaerobic bacteria.

abstract：:Knowledge of host specificity, combined with genomic sequencing of Giardia and Cryptosporidium spp., has demonstrated a microbial source tracking (MST) utility for these common waterborne microbes. To explore the source attribution potential of these pathogens, water samples were collected in a mixed rural-urban water...

journal_title：Applied and environmental microbiology

authors： Prystajecky N,Huck PM,Schreier H,Isaac-Renton JL

更新日期：2014-04-01 00:00:00

abstract：:Colonization of apple leaves by the yeastlike fungus Aureobasidium pullulans was followed quantitatively and spatially at a microscale level throughout two growing seasons. Ten field leaves were sampled on 11 dates in 2003 and 15 dates in 2004. Using an A. pullulans-specific fluorescence in situ hybridization probe an...

journal_title：Applied and environmental microbiology

authors： McGrath MJ,Andrews JH

更新日期：2006-09-01 00:00:00

abstract：:Minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose cell walls are particularly vulnerable. Little is known about the roles that different structural...

journal_title：Applied and environmental microbiology

authors： Tan MS,Rahman S,Dykes GA

更新日期：2015-11-13 00:00:00

abstract：:Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spec...

journal_title：Applied and environmental microbiology

authors： Rebuffo CA,Schmitt J,Wenning M,von Stetten F,Scherer S

更新日期：2006-02-01 00:00:00

abstract：:Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitat...

journal_title：Applied and environmental microbiology

authors： Hammes F,Boon N,Vital M,Ross P,Magic-Knezev A,Dignum M

更新日期：2011-02-01 00:00:00

abstract：:Xylanase activity from naturally occurring color variants of Aureobasidium pullulans was associated with extracellular monomeric proteins of 20 to 21 kilodaltons. Xylanase represented nearly half the total extracellular protein, with a yield of up to 0.3 g of xylanase per liter. The specific activity of partially puri...

journal_title：Applied and environmental microbiology

authors： Leathers TD

更新日期：1986-11-01 00:00:00

abstract：:Phytoplankton replace phosphorus-containing lipids (P-lipids) with non-P analogues, boosting growth in P-limited oceans. In the model diatom Thalassiosira pseudonana, the substitution dynamics of lipid headgroups are well described, but those of the individual lipids, differing in fatty acid composition, are unknown. ...

journal_title：Applied and environmental microbiology

authors： Hunter JE,Brandsma J,Dymond MK,Koster G,Moore CM,Postle AD,Mills RA,Attard GS

更新日期：2018-03-01 00:00:00

abstract：:We examined the effect of different sediment types on the staining effectiveness of the fluorochrome DAPI (4'-6-diamidino-2-phenylindole dihydrochloride) over a wide range of concentrations and on the masking effect of sediment particles on DAPI-stained bacteria. Sediment type greatly affects the staining efficiency o...

journal_title：Applied and environmental microbiology

authors： Schallenberg M,Kalff J,Rasmussen JB

更新日期：1989-05-01 00:00:00

abstract：:Legionella pneumophila, the agent of Legionnaires' disease, is an intracellular parasite of aquatic amoebae and human macrophages. A key factor for L. pneumophila in intracellular infection is its type II protein secretion system (Lsp). In order to more completely define Lsp output, we recently performed a proteomic a...

journal_title：Applied and environmental microbiology

authors： Rossier O,Dao J,Cianciotto NP

更新日期：2008-02-01 00:00:00

abstract：:[This corrects the article on p. 1301 in vol. 50.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1986-03-01 00:00:00

abstract：:A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these...

journal_title：Applied and environmental microbiology

authors： Morotomi M,Ohno T,Mutai M

更新日期：1988-05-01 00:00:00

abstract：:Pot experiments showed that Rhizobium japonicum cells entrapped in a polyacrylamide gel could be used as an inoculant for soybeans and compared favorably to laboratory-made peat base inoculant containing the same bacterial strain. ...

journal_title：Applied and environmental microbiology

authors： Dommergues YR,Diem HG,Divies C

更新日期：1979-04-01 00:00:00

abstract：:We studied the growth of six culturable bacterial lineages from coastal North Sea picoplankton in environmental samples under different incubation conditions. The grazing pressure of heterotrophic nanoflagellates (HNF) was reduced either by double prefiltration through 0.8- micro m-pore-size filters or by 10-fold dilu...

journal_title：Applied and environmental microbiology

authors： Beardsley C,Pernthaler J,Wosniok W,Amann R

更新日期：2003-05-01 00:00:00

abstract：:We isolated thermotolerant Gluconobacter strains that are able to produce 5-keto-d-gluconic acid (5KGA) at 37 degrees C, a temperature at which regular mesophilic 5KGA-producing strains showed much less growth and 5KGA production. The thermotolerant strains produced 2KGA as the major product at both 30 and 37 degrees ...

journal_title：Applied and environmental microbiology

authors： Saichana I,Moonmangmee D,Adachi O,Matsushita K,Toyama H

更新日期：2009-07-01 00:00:00

abstract：:A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes. ...

journal_title：Applied and environmental microbiology

authors： Herman LM,De Block JH,Moermans RJ

更新日期：1995-02-01 00:00:00

abstract：:Anoxic sediments from Rotsee (Switzerland) were analyzed for the presence and diversity of methanogens by using molecular tools and for methanogenic activity by using radiotracer techniques, in addition to the measurement of chemical profiles. After PCR-assisted sequence retrieval of the 16S rRNA genes (16S rDNA) from...

journal_title：Applied and environmental microbiology

authors： Zepp Falz K,Holliger C,Grosskopf R,Liesack W,Nozhevnikova AN,Müller B,Wehrli B,Hahn D

更新日期：1999-06-01 00:00:00

abstract：:The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...

journal_title：Applied and environmental microbiology

authors： Gagnevin L,Leach JE,Pruvost O

更新日期：1997-01-01 00:00:00

abstract：:Two anaerobic bacteria were isolated from polyethylene glycol (PEG)-degrading, methanogenic, enrichment cultures obtained from a municipal sludge digester. One isolate, identified as Desulfovibrio desulfuricans (strain DG2), metabolized oligomers ranging from ethylene glycol (EG) to tetraethylene glycol. The other iso...

journal_title：Applied and environmental microbiology

authors： Dwyer DF,Tiedje JM

更新日期：1986-10-01 00:00:00

abstract：:The currently used microbial decontamination method for spacecraft and components uses dry-heat microbial reduction at temperatures of >110°C for extended periods to prevent the contamination of extraplanetary destinations. This process is effective and reproducible, but it is also long and costly and precludes the us...

journal_title：Applied and environmental microbiology

authors： Pottage T,Macken S,Giri K,Walker JT,Bennett AM

更新日期：2012-06-01 00:00:00

abstract：:For efficient generation of high-affinity protein-based binding molecules, fast and reliable downstream characterization platforms are needed. In this work, we have explored the use of staphylococcal cell surface display together with flow cytometry for affinity characterization of candidate affibody molecules directl...

journal_title：Applied and environmental microbiology

authors： Löfblom J,Sandberg J,Wernérus H,Ståhl S

更新日期：2007-11-01 00:00:00

abstract：:Lactococcus lactis is a prokaryotic microorganism with great importance as a culture starter and has become the model species among the lactic acid bacteria. The long and safe history of use of L. lactis in dairy fermentations has resulted in the classification of this species as GRAS (General Regarded As Safe) or QPS...

journal_title：Applied and environmental microbiology

authors： Ladero V,Rattray FP,Mayo B,Martín MC,Fernández M,Alvarez MA

更新日期：2011-09-01 00:00:00

abstract：:Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and acidic heathland...

journal_title：Applied and environmental microbiology

authors： Herrmann M,Saunders AM,Schramm A

更新日期：2009-05-01 00:00:00

abstract：:Transfer of plasmid-borne antibiotic resistance genes in Escherichia coli wild-type strains is possible by transformation under naturally occurring conditions in oligotrophic, aquatic environments containing physiologic concentrations of calcium. In contrast, transformation is suppressed in nitrogen-rich body fluids l...

journal_title：Applied and environmental microbiology

authors： Woegerbauer M,Jenni B,Thalhammer F,Graninger W,Burgmann H

更新日期：2002-01-01 00:00:00

abstract：:We studied the role of the regulatory gene aflR and its product, AflR, in the biosynthesis of aflatoxin in Aspergillus. Western blot and enzyme-linked immunosorbent assay analyses revealed that aflatoxin B1 accumulation was directly related to AflR expression and was regulated by various environmental and nutritional ...

journal_title：Applied and environmental microbiology

authors： Liu BH,Chu FS

更新日期：1998-10-01 00:00:00

abstract：:The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses. Recently in the United States, dramatic honeybee losses (colony collapse disorder) have been reported; however, there remain...

journal_title：Applied and environmental microbiology

authors： Highfield AC,El Nagar A,Mackinder LC,Noël LM,Hall MJ,Martin SJ,Schroeder DC

更新日期：2009-11-01 00:00:00

abstract：:Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid...

journal_title：Applied and environmental microbiology

authors： Morlino GB,Tizzani L,Fleer R,Frontali L,Bianchi MM

更新日期：1999-11-01 00:00:00

abstract：:Protease-negative variants were shown to outcompete the wild-type strains of Streptococcus cremoris E(8), HP, and Wg(2) at pH values higher than 6.0 in milk. For S. cremoris E(8) this process was studied in more detail. At lower pH values the wild type had a selective advantage. This pH-dependent selection was not fou...

journal_title：Applied and environmental microbiology

authors： Hugenholtz J,Splint R,Konings WN,Veldkamp H

更新日期：1987-02-01 00:00:00

abstract：:We report the identification of novel archaea living in close association with bacteria in the cold (approximately 10 degrees C) sulfurous marsh water of the Sippenauer Moor near Regensburg, Bavaria, Germany. These microorganisms form a characteristic, macroscopically visible structure, morphologically comparable to a...

journal_title：Applied and environmental microbiology

authors： Rudolph C,Wanner G,Huber R

更新日期：2001-05-01 00:00:00

abstract：:Plankton to which cells of Vibrio cholerae O1 and/or O139 were attached was introduced into 0.5% Instant Ocean microcosms maintained at 25 degrees C. The bulk of the plankton and associated particulates was removed with a filter constructed from either nylon net and one of several different types of sari material, the...

journal_title：Applied and environmental microbiology

authors： Huq A,Xu B,Chowdhury MA,Islam MS,Montilla R,Colwell RR

更新日期：1996-07-01 00:00:00

abstract：:Crude extracts from 14 species of sulfate-reducing bacteria comprising the genera Desulfovibrio, Desulfotomaculum, Desulfobulbus, and Desulfosarcina and from three species of sulfide-oxidizing bacteria were tested in an enzyme-linked immunosorbent assay with polyclonal antisera to adenosine 5'-phosphosulfate reductase...

journal_title：Applied and environmental microbiology

authors： Odom JM,Jessie K,Knodel E,Emptage M

更新日期：1991-03-01 00:00:00