Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers.

Abstract:

:A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.

journal_name

Appl Environ Microbiol

authors

Herman LM,De Block JH,Moermans RJ

doi

10.1128/AEM.61.2.817-819.1995

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

817-9

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

61

pub_type

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