Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH.

abstract：:The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...

journal_title：Applied and environmental microbiology

authors： Tan PS,van Kessel TA,van de Veerdonk FL,Zuurendonk PF,Bruins AP,Konings WN

更新日期：1993-05-01 00:00:00

abstract：:Two strains of 1,1,1-trichloroethane (TCA)-degrading bacteria, TA5 and TA27, were isolated from soil and identified as Mycobacterium spp. Strains TA5 and TA27 could degrade 25 and 75 mg. liter of TCA(-1) cometabolically in the presence of ethane as a carbon source, respectively. The compound 2,2,2-trichloroethanol was...

journal_title：Applied and environmental microbiology

authors： Yagi O,Hashimoto A,Iwasaki K,Nakajima M

更新日期：1999-10-01 00:00:00

abstract：:The diversity of microcystin-producing cyanobacteria in the western basin of Lake Erie was studied using sequence analysis of mcyA gene fragments. Distinct populations of potentially toxic Microcystis and Planktothrix were found in spatially isolated locations. This study highlights previously undocumented diversity o...

journal_title：Applied and environmental microbiology

authors： Rinta-Kanto JM,Wilhelm SW

更新日期：2006-07-01 00:00:00

abstract：:Methanogenic bacteria, which are presently identified on the basis of cell morphology and substrate conversion to CH(4), can be differentiated from nonmethanogens and identified in pure or mixed culture on the basis of their autofluorescence under ultraviolet illumination. ...

journal_title：Applied and environmental microbiology

authors： Mink RW,Dugan PR

更新日期：1977-03-01 00:00:00

abstract：:Resources from the Sinorhizobium meliloti Rm1021 open reading frame (ORF) plasmid libraries were used in a medium-throughput method to construct a set of 50 overlapping deletion mutants covering all of the Rm1021 pSymA megaplasmid except the replicon region. Each resulting pSymA derivative carried a defined deletion o...

journal_title：Applied and environmental microbiology

authors： Yurgel SN,Mortimer MW,Rice JT,Humann JL,Kahn ML

更新日期：2013-03-01 00:00:00

abstract：:Extracellular DNA (eDNA) is a biofilm component that contributes to the formation and structural stability of biofilms. Streptococcus mutans, a major cariogenic bacterium, induces eDNA-dependent biofilm formation under specific conditions. Since cell death can result in the release and accumulation of DNA, the dead ce...

journal_title：Applied and environmental microbiology

authors： Nagasawa R,Yamamoto T,Utada AS,Nomura N,Obana N

更新日期：2020-11-10 00:00:00

abstract：:We applied HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ PCR for the routine detection of specific bacterial cells at the single-cell level. PCR was performed on glass slides with digoxigenin-labeled dUTP. The digoxigenin-labeled PCR products were detected with alkaline phosphatase-lab...

journal_title：Applied and environmental microbiology

authors： Tani K,Kurokawa K,Nasu M

更新日期：1998-04-01 00:00:00

abstract：:Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

journal_title：Applied and environmental microbiology

authors： Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

更新日期：2003-02-01 00:00:00

abstract：:The microbial degradation of tensile test pieces made of poly(3-hydroxybutyrate) [P(3HB)] or a copolymer of 90% 3-hydroxybutyric acid and 10% 3-hydroxyvaleric acid was studied in soils incubated at a constant temperature of 15, 28, or 40 degrees C for up to 200 days. In addition, hydrolytic degradation in sterile buff...

journal_title：Applied and environmental microbiology

authors： Mergaert J,Webb A,Anderson C,Wouters A,Swings J

更新日期：1993-10-01 00:00:00

abstract：:Bacillus megaterium P1, a bacterial strain capable of hydrolyzing chitosan, was isolated from soil samples. Chitosan-degrading activity was induced by chitosan but not by its constituent d-glucosamine. Extracellular secretion of chitosanase reached levels corresponding to 1 U/ml under optimal conditions. Three chitosa...

journal_title：Applied and environmental microbiology

authors： Pelletier A,Sygusch J

更新日期：1990-04-01 00:00:00

abstract：:The gene cluster that codes for feedback-resistant aspartate kinase (lysCalpha and lysCbeta) and aspartate semialdehyde dehydrogenase (asd) was cloned from a mutant strain of Corynebacterium glutamicum. Its functional analysis by subcloning, enzyme assays, and type of aspartate kinase regulation enabled the isolation ...

journal_title：Applied and environmental microbiology

authors： Cremer J,Eggeling L,Sahm H

更新日期：1991-06-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the ...

journal_title：Applied and environmental microbiology

authors： Platteeuw C,Hugenholtz J,Starrenburg M,van Alen-Boerrigter I,de Vos WM

更新日期：1995-11-01 00:00:00

abstract：:The viability of starved or dying Saccharomyces cerevisiae Y5 cells was extended for up to 1,500 h by adding osmotic supports (0.6 M sorbitol or mannitol) to the starvation media. Replacement of these polyols with 0.3 M KCl, 0.5 M MgSO4, or 0.2 M MgCl2 postponed the death of cells incubated in N-free medium, but accel...

journal_title：Applied and environmental microbiology

authors： Peled ON

更新日期：1985-09-01 00:00:00

abstract：:In this study, the effect of individual lecithin phospholipids on the antimicrobial properties of eugenol against Escherichia coli C600 was investigated. We tested five major phospholipids common in soy or egg lecithin (1,2-dihexadecanoyl-sn-glycero-3-phosphocholine [DPPC], 1,2-dioctadecanoyl-sn-glycero-3-phosphocholi...

journal_title：Applied and environmental microbiology

authors： Zhang H,Dudley EG,Harte F

更新日期：2017-10-17 00:00:00

abstract：:Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

journal_title：Applied and environmental microbiology

authors： Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

更新日期：2019-01-09 00:00:00

abstract：:Lipid accumulation and fatty acid composition in Candida 107 have been studied using a two-stage continuous culture system in which the first vessel was run under carbon-limited conditions and then the entire output was passed into a second vessel, where lipid accumulation was stimulated by adding only glucose. Maximu...

journal_title：Applied and environmental microbiology

authors： Hall MJ,Ratledge C

更新日期：1977-03-01 00:00:00

abstract：:Five strains of Aspergillus fumigatus (A, B, D, H, and K) isolated from sawmills were used to expose groups of three rats by intratracheal intubation. The dose was 10(9) spores per rat. At 48 h after administration, two rats from the D group and all rats from the K group died with symptoms of strong dyspnea and tachyp...

journal_title：Applied and environmental microbiology

authors： Land CJ,Sostarić B,Fuchs R,Lundström H,Hult K

更新日期：1989-11-01 00:00:00

abstract：:Effective wastewater management is crucial to ensure the safety of water reuse projects and effluent discharge into surface waters. Multiple studies have demonstrated that municipal wastewater treatment with conventional activated sludge processes is inefficient for the removal of a wide spectrum of viruses in sewage....

journal_title：Applied and environmental microbiology

authors： Chacón L,Barrantes K,Santamaría-Ulloa C,Solano M,Reyes L,Taylor L,Valiente C,Symonds EM,Achí R

更新日期：2020-08-18 00:00:00

abstract：:Toxin-producing isolates of Bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. The toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic ...

journal_title：Applied and environmental microbiology

authors： Salkinoja-Salonen MS,Vuorio R,Andersson MA,Kämpfer P,Andersson MC,Honkanen-Buzalski T,Scoging AC

更新日期：1999-10-01 00:00:00

abstract：:To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and ...

journal_title：Applied and environmental microbiology

authors： Shin H,Lee JH,Yoon H,Kang DH,Ryu S

更新日期：2014-01-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized antimicrobial peptides with substantial posttranslational modifications. They are characterized by the unique amino acids lanthionine and methyllanthionine, which are introduced by dehydration of Ser/Thr residues and linkage of the resulting dehydrated amino acids with Cys resi...

journal_title：Applied and environmental microbiology

authors： Basi-Chipalu S,Dischinger J,Josten M,Szekat C,Zweynert A,Sahl HG,Bierbaum G

更新日期：2015-05-15 00:00:00

abstract：:We report a method which combines epifluorescence microscopy and microautoradiography to determine both the total number of microorganisms in natural water populations and those individual organisms active in the uptake of specific substrates. After incubation with H-labeled substrate, the sample is filtered and, whil...

journal_title：Applied and environmental microbiology

authors： Tabor PS,Neihof RA

更新日期：1982-10-01 00:00:00

abstract：:Root-colonizing, saprophytic fluorescent pseudomonads of the Pseudomonas putida-P. fluorescens group express similar levels of catalase and superoxide dismutase activities during growth on a sucrose- and amino acid-rich medium. Increased specific activities of catalase but not superoxide dismutase were observed during...

journal_title：Applied and environmental microbiology

authors： Katsuwon J,Anderson AJ

更新日期：1990-11-01 00:00:00

abstract：:Erwinia carotovora subsp. atroseptica, E. carotovora subsp. betavasculorum, and E. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt), and the quorum-sensing signal N-acyl-homoserine lactone (AHL) at 28 de...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Chatterjee A,Cui Y,Chatterjee AK

更新日期：2005-08-01 00:00:00

abstract：:Upstream of the Streptomyces coelicolor A3(2) chitinase G gene, a small gene (named chb3) is located whose deduced product shares 37% identical amino acids with the previously described CHB1 protein from Streptomyces olivaceoviridis. The chb3 gene and its upstream region were cloned in a multicopy vector and transform...

journal_title：Applied and environmental microbiology

authors： Saito A,Miyashita K,Biukovic G,Schrempf H

更新日期：2001-03-01 00:00:00

abstract：:O island 48 (OI-48) of Escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (Te(r)), and putative adhesins Iha and AIDA-1. The functions of these clusters in enterohemorrhagic E. coli (EHEC) O157:H7 infection are unknown. Deletion mutants for these three regions were con...

journal_title：Applied and environmental microbiology

authors： Yin X,Wheatcroft R,Chambers JR,Liu B,Zhu J,Gyles CL

更新日期：2009-09-01 00:00:00

abstract：:Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic microbes that are found in a broad range of aquatic environments. Although potentially significant to the microbial ecology and biogeochemistry of marine ecosystems, their abundance and genetic diversity and the environmental variables that regulate...

journal_title：Applied and environmental microbiology

authors： Ritchie AE,Johnson ZI

更新日期：2012-04-01 00:00:00

abstract：:Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are highly motile Gram-negative predatory bacteria with the potential of being used as biocontrol agents or living antibiotics. It was suggested previously that sugar-binding proteins play a role in M. aeruginosavorus and B. bacteriovorus host specificity and p...

journal_title：Applied and environmental microbiology

authors： Dashiff A,Keeling TG,Kadouri DE

更新日期：2011-04-01 00:00:00