Abstract:
:A bacterial biosensor for benzene, toluene, and similar compounds has been constructed, characterized, and field tested on contaminated water and soil. The biosensor is based on a plasmid incorporating the transcriptional activator xylR from the TOL plasmid of Pseudomonas putida mt-2. The XylR protein binds a subset of toluene-like compounds and activates transcription at its promoter, Pu. A reporter plasmid was constructed by placing the luc gene for firefly luciferase under the control of XylR and Pu. When Escherichia coli cells were transformed with this plasmid vector, luminescence from the cells was induced in the presence of benzene, toluene, xylenes, and similar molecules. Accurate concentration dependencies of luminescence were obtained and exhibited K1/2 values ranging from 39.0 +/- 3.8 microM for 3-xylene to 2,690 +/- 160 microM for 3-methylbenzylalcohol (means +/- standard deviations). The luminescence response was specific for only toluene-like molecules that bind to and activate XylR. The biosensor cells were field tested on deep aquifer water, for which contaminant levels were known, and were able to accurately detect toluene derivative contamination in this water. The biosensor cells were also shown to detect BETX (benzene, toluene, and xylene) contamination in soil samples. These results demonstrate the capability of such a bacterial biosensor to accurately measure environmental contaminants and suggest a potential for its inexpensive application in field-ready assays.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Willardson BM,Wilkins JF,Rand TA,Schupp JM,Hill KK,Keim P,Jackson PJdoi
10.1128/AEM.64.3.1006-1012.1998subject
Has Abstractpub_date
1998-03-01 00:00:00pages
1006-12issue
3eissn
0099-2240issn
1098-5336journal_volume
64pub_type
杂志文章abstract::We constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated pHR106, by combining desired segments of three plasmids: an Escherichia coli plasmid (pSL100) which provides a multiple cloning site, a Clostridium perfringens plasmid (pJU122) which provides a clostridial origin of replication, and an E. coli ...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.54.1.268-270.1988
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doi:10.1128/AEM.46.3.758-761.1983
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pub_type: 杂志文章
doi:10.1128/AEM.44.1.255-257.1982
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.1.74-82.1993
更新日期:1993-01-01 00:00:00
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doi:10.1128/AEM.31.2.313-315.1976
更新日期:1976-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.63.9.3494-3498.1997
更新日期:1997-09-01 00:00:00
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doi:10.1128/AEM.41.2.511-517.1981
更新日期:1981-02-01 00:00:00
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更新日期:1997-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2015-06-01 00:00:00