Biochemical fingerprinting of water coliform bacteria, a new method for measuring phenotypic diversity and for comparing different bacterial populations.

abstract：:The control of promoter activity by oxygen availability appears to be an intriguing system for heterologous protein production. In fact, during cell growth in a bioreactor, an oxygen shortage is easily obtained simply by interrupting the air supply. The purpose of our work was to explore the possible use of hypoxic in...

journal_title：Applied and environmental microbiology

authors： Camattari A,Bianchi MM,Branduardi P,Porro D,Brambilla L

更新日期：2007-02-01 00:00:00

abstract：:The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...

journal_title：Applied and environmental microbiology

authors： Gagnevin L,Leach JE,Pruvost O

更新日期：1997-01-01 00:00:00

abstract：:Characterization by partial 16S rRNA gene sequencing, ribotyping, and green fluorescent protein-based nisin bioassay revealed that 6 of 20 human milk samples contained nisin-producing Lactococcus lactis bacteria. This suggests that the history of humans consuming nisin is older than the tradition of consuming fermente...

journal_title：Applied and environmental microbiology

authors： Beasley SS,Saris PE

更新日期：2004-08-01 00:00:00

abstract：:Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified...

journal_title：Applied and environmental microbiology

authors： Schoenborn L,Yates PS,Grinton BE,Hugenholtz P,Janssen PH

更新日期：2004-07-01 00:00:00

abstract：:This study examined the inactivation of human norovirus (HuNoV) GI.1 and GII.4 by chlorine under conditions mimicking sewage treatment. Using a porcine gastric mucin-magnetic bead (PGM-MB) assay, no statistically significant loss in HuNoV binding (inactivation) was observed for secondary effluent treatments of ≤25 ppm...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Fay JP,Calci K,Pouillot R,Woods J,Chen H,Niemira BA,Van Doren JM

更新日期：2017-11-16 00:00:00

abstract：:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension ana...

journal_title：Applied and environmental microbiology

authors： Labarre C,Diviès C,Guzzo J

更新日期：1996-12-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, respectively). The sp...

journal_title：Applied and environmental microbiology

authors： Heuer H,Krsek M,Baker P,Smalla K,Wellington EM

更新日期：1997-08-01 00:00:00

abstract：:Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strai...

journal_title：Applied and environmental microbiology

authors： Vesper SJ

更新日期：1987-07-01 00:00:00

abstract：:Transpositional mutagenesis of Thiobacillus novellus by Tn501 was achieved by means of the incompatibility of IncP plasmids. Tn501 insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO(2). Oxidation rates for...

journal_title：Applied and environmental microbiology

authors： Davidson MS,Roy P,Summers AO

更新日期：1985-06-01 00:00:00

abstract：:One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector co...

journal_title：Applied and environmental microbiology

authors： Gonella E,Negri I,Marzorati M,Mandrioli M,Sacchi L,Pajoro M,Crotti E,Rizzi A,Clementi E,Tedeschi R,Bandi C,Alma A,Daffonchio D

更新日期：2011-02-01 00:00:00

abstract：:A number of Micromonospora strains isolated from the water column, sediment, and cellulose baits placed in freshwater lakes were shown to be able to degrade cellulose in lake water without any addition of nutrients. A selective isolation method was also developed to demonstrate that CFU arose from both spores and hyph...

journal_title：Applied and environmental microbiology

authors： de Menezes AB,Lockhart RJ,Cox MJ,Allison HE,McCarthy AJ

更新日期：2008-11-01 00:00:00

abstract：:The development of resistance in foodborne pathogens to food preservation techniques is an issue of increasing concern, especially in minimally processed foods where safety relies on hurdle technology. In this context, mild heat can be used in combination with so-called nonthermal processes, such as high hydrostatic p...

journal_title：Applied and environmental microbiology

authors： Gayán E,Cambré A,Michiels CW,Aertsen A

更新日期：2016-10-27 00:00:00

abstract：:Mutacin 1140 belongs to the epidermin family of type AI lantibiotics. This family has a broad spectrum of activity against Gram-positive bacteria. The binding of mutacin 1140 to lipid II leads to the inhibition of cell wall synthesis. Pharmacokinetic experiments with type AI lantibiotics are generally discouraging for...

journal_title：Applied and environmental microbiology

authors： Geng M,Smith L

更新日期：2018-07-17 00:00:00

abstract：:A Bacillus sp. capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from Cape Canerval soil samples. The organism was isolated on a glucose-mineral salts medium with phosphate deleted. Anaerobic cultivation of this isolate resulted in decreases in the hypophosphite or phosphite conc...

journal_title：Applied and environmental microbiology

authors： Foster TL,Winans L Jr,Helms SJ

更新日期：1978-05-01 00:00:00

abstract：:The prophage-encoded Shiga toxin is a major virulence factor in Stx-producing Escherichia coli (STEC). Toxin production and phage production are linked and occur after induction of the RecA-dependent SOS response. However, food-related stress and Stx-prophage induction have not been studied at the single-cell level. T...

journal_title：Applied and environmental microbiology

authors： Fang Y,Mercer RG,McMullen LM,Gänzle MG

更新日期：2017-09-15 00:00:00

abstract：:The lipopeptide, surfactin, is produced by Bacillus subtilis. A study has been made on large-scale production of this surfactant. A good yield was obtained from a glucose substrate fermentation by continuously removing the product by foam fractionation. The surfactin could be easily recovered from the collapsed foam b...

journal_title：Applied and environmental microbiology

authors： Cooper DG,Macdonald CR,Duff SJ,Kosaric N

更新日期：1981-09-01 00:00:00

abstract：:The need for protozoa for the proliferation of Legionella pneumophila in aquatic habitats is still not fully understood and is even questioned by some investigators. This study shows the in vivo growth of L. pneumophila in protozoa in aquatic biofilms developing at high concentrations on plasticized polyvinyl chloride...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Wullings BA,Akkermans AD,Beumer RR,van der Kooij D

更新日期：2004-11-01 00:00:00

abstract：:Here, we report the genome of one gammaproteobacterial member of the gut microbiota, for which we propose the name "Candidatus Schmidhempelia bombi," that was inadvertently sequenced alongside the genome of its host, the bumble bee, Bombus impatiens. This symbiont is a member of the recently described bacterial order ...

journal_title：Applied and environmental microbiology

authors： Martinson VG,Magoc T,Koch H,Salzberg SL,Moran NA

更新日期：2014-07-01 00:00:00

abstract：:Effects of the sesquiterpene farnesol (3,7,11-trimethyl-2,6,10-dodecatrien-1-ol) and the sesquiterpene derivative geosmin (1,10-trans-dimethyl-trans-9-decalol) were investigated in a geosmin-producing actinomycete, Streptomyces tendae. Exposure to 300 microM farnesol reduced biomass (fresh matter) accumulation by 97% ...

journal_title：Applied and environmental microbiology

authors： Dionigi CP,Millie DF,Johnsen PB

更新日期：1991-12-01 00:00:00

abstract：:Our goal was to develop a field soil biodegradation assay using (13)C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived (13)C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soi...

journal_title：Applied and environmental microbiology

authors： Padmanabhan P,Padmanabhan S,DeRito C,Gray A,Gannon D,Snape JR,Tsai CS,Park W,Jeon C,Madsen EL

更新日期：2003-03-01 00:00:00

abstract：:Mechanisms for Fe(III) oxide reduction were investigated in Geothrix fermentans, a dissimilatory Fe(III)-reducing microorganism found within the Fe(III) reduction zone of subsurface environments. Culture filtrates of G. fermentans stimulated the reduction of poorly crystalline Fe(III) oxide by washed cell suspensions,...

journal_title：Applied and environmental microbiology

authors： Nevin KP,Lovley DR

更新日期：2002-05-01 00:00:00

abstract：:A dissimilatory Fe(III)- and Mn(IV)-reducing bacterium was isolated from bottom sediments of the Great Bay estuary, New Hampshire. The isolate was a facultatively anaerobic gram-negative rod which did not appear to fit into any previously described genus. It was temporarily designated strain BrY. BrY grew anaerobicall...

journal_title：Applied and environmental microbiology

authors： Caccavo F,Blakemore RP,Lovley DR

更新日期：1992-10-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:A rare strain of Clostridium botulinum subtype Ab was isolated from a canned macrobiotic food suspected of being linked to a fatal case of food-borne botulism. The strain was recovered and identified by conventional methods modified by the inclusion of a PCR assay (G. Franciosa, J.L. Ferreira, and C.L. Hatheway, J. Cl...

journal_title：Applied and environmental microbiology

authors： Franciosa G,Fenicia L,Pourshaban M,Aureli P

更新日期：1997-03-01 00:00:00

abstract：:In this study, we developed a large-scale screening of bacterial strains in order to identify novel candidate probiotics with immunomodulatory properties. For this, 158 strains, including a majority of lactic acid bacteria (LAB), were screened by two different cellular models: tumor necrosis factor alpha (TNF-α)-activ...

journal_title：Applied and environmental microbiology

authors： Kechaou N,Chain F,Gratadoux JJ,Blugeon S,Bertho N,Chevalier C,Le Goffic R,Courau S,Molimard P,Chatel JM,Langella P,Bermúdez-Humarán LG

更新日期：2013-03-01 00:00:00

abstract：:The diversities leaf-associated bacteria on nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans were evaluated by clone library analyses of the 16S rRNA gene. To analyze the impact of nitrogen fertilization on the bacterial leaf community, soybeans were treated with standard nitr...

journal_title：Applied and environmental microbiology

authors： Ikeda S,Anda M,Inaba S,Eda S,Sato S,Sasaki K,Tabata S,Mitsui H,Sato T,Shinano T,Minamisawa K

更新日期：2011-03-01 00:00:00

abstract：:Similar populations of hypochlorite-treated spores were enumerated from two crops of Clostridium botulinum 12885A produced by the same procedure; however, germination required different L-alanine concentrations. Lactate permitted the germination of spores from both crops with suboptimal L-alanine concentrations. The d...

journal_title：Applied and environmental microbiology

authors： Foegeding PM,Busta FF

更新日期：1983-04-01 00:00:00

abstract：:In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture ...

journal_title：Applied and environmental microbiology

authors： Providenti MA,Shaye RE,Lynes KD,McKenna NT,O'brien JM,Rosolen S,Wyndham RC,Lambert IB

更新日期：2006-04-01 00:00:00

abstract：:A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon in...

journal_title：Applied and environmental microbiology

authors： Bouhenni R,Gehrke A,Saffarini D

更新日期：2005-08-01 00:00:00