Respiration of 13C-labeled substrates added to soil in the field and subsequent 16S rRNA gene analysis of 13C-labeled soil DNA.

Abstract:

:Our goal was to develop a field soil biodegradation assay using (13)C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived (13)C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. The new field-based assay involved the release of (13)C-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil plots, installation of open-bottom glass chambers that covered the soil, and analysis of samples of headspace gases for (13)CO(2) respiration by gas chromatography/mass spectrometry (GC/MS). We verified that the GC/MS procedure was capable of assessing respiration of the four substrates added (50 ppm) to 5 g of soil in sealed laboratory incubations. Next, we determined background levels of (13)CO(2) emitted from naturally occurring soil organic matter to chambers inserted into our field soil test plots. We found that the conservative tracer, SF(6), that was injected into the headspace rapidly diffused out of the soil chamber and thus would be of little value for computing the efficiency of retaining respired (13)CO(2). Field respiration assays using all four compounds were completed. Background respiration from soil organic matter interfered with the documentation of in situ respiration of the slowly metabolized (caffeine) and sparingly soluble (naphthalene) compounds. Nonetheless, transient peaks of (13)CO(2) released in excess of background were found in glucose- and phenol-treated soil within 8 h. Cesium-chloride separation of (13)C-labeled soil DNA was followed by PCR amplification and sequencing of 16S rRNA genes from microbial populations involved with (13)C-substrate metabolism. A total of 29 full sequences revealed that active populations included relatives of Arthrobacter, Pseudomonas, Acinetobacter, Massilia, Flavobacterium, and Pedobacter spp. for glucose; Pseudomonas, Pantoea, Acinetobacter, Enterobacter, Stenotrophomonas, and Alcaligenes spp. for phenol; Pseudomonas, Acinetobacter, and Variovorax spp. for naphthalene; and Acinetobacter, Enterobacter, Stenotrophomonas, and Pantoea spp. for caffeine.

journal_name

Appl Environ Microbiol

authors

Padmanabhan P,Padmanabhan S,DeRito C,Gray A,Gannon D,Snape JR,Tsai CS,Park W,Jeon C,Madsen EL

doi

10.1128/aem.69.3.1614-1622.2003

keywords:

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

1614-22

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

69

pub_type

杂志文章
  • Effect of visible light on progressive dormancy of Escherichia coli cells during the survival process in natural fresh water.

    abstract::Some effects of visible light on the survival of Escherichia coli in waters of the Butrón river were studied by comparing illuminated and nonilluminated systems. The following count methods were used: CFU on a selective medium (eosin-methylene blue agar), CFU on a medium of recuperation (Trypticase soy agar with yeast...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.1.246-251.1989

    authors: Barcina I,González JM,Iriberri J,Egea L

    更新日期:1989-01-01 00:00:00

  • Formation of Crystalline delta-Endotoxin or Poly-beta-Hydroxybutyric Acid Granules by Asporogenous Mutants of Bacillus thuringiensis.

    abstract::Parental strains and asporogenous mutants of Bacillus thuringiensis subspp. kurstaki and aizawai produced high yields of delta-endotoxin on M medium, which contained 330 mug of potassium per ml, but not on ST and ST-a media, each of which contained only 11 mug of potassium per ml. On ST and ST-a media, refractile gran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.6.1473-1480.1982

    authors: Wakisaka Y,Masaki E,Nishimoto Y

    更新日期:1982-06-01 00:00:00

  • Stable isotopic studies of n-alkane metabolism by a sulfate-reducing bacterial enrichment culture.

    abstract::Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8174-8182.2005

    authors: Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

    更新日期:2005-12-01 00:00:00

  • Molecular characterization of commensal Escherichia coli adapted to different compartments of the porcine gastrointestinal tract.

    abstract::The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01688-12

    authors: Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

    更新日期:2012-10-01 00:00:00

  • Microbial rRNA Synthesis and Growth Compared through Quantitative Stable Isotope Probing with H218O.

    abstract::Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a so...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02441-17

    authors: Papp K,Hungate BA,Schwartz E

    更新日期:2018-04-02 00:00:00

  • Symbiotic Cellulose Degradation in Green Turtles, Chelonia mydas L.

    abstract::A postgastric, fermentative breakdown of structural plant tissue was demonstrated for green turtles. About 90% cellulose was hydrolyzed. Bacterial and protozoan numbers compared with those of the rumen. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.2.348-350.1979

    authors: Fenchel TM,McRoy CP,Ogden JC,Parker P,Rainey WE

    更新日期:1979-02-01 00:00:00

  • Production of deoxynivalenol by Fusarium isolates from samples of wheat associated with a human mycotoxicosis outbreak and from sorghum cultivars.

    abstract::Fusarium isolates from specific diseased sorghum plants and rain-soaked wheat and wheat flour associated with human mycotoxicosis in India have been screened for their toxigenic potential. Of the 322 isolates screened, 11 isolates were found to produce deoxynivalenol in concentrations ranging from 0.01 to 186 microgra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.10.2619-2620.1989

    authors: Ramakrishna Y,Bhat RV,Ravindranath V

    更新日期:1989-10-01 00:00:00

  • Fungal Metabolism of n-Alkylbenzenes.

    abstract::Isolates of Paecilomyces, Verticillium, Beauveria, and Penicillium species were tested for ability to metabolize a variety of n-alkylbenzenes. Minimum side chain lengths were required for metabolism of these substrates. These were C(4) for the Paecilomyces sp., C(8) for the Verticillium sp., and C(9) for the other two...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.2.435-437.1986

    authors: Fedorak PM,Westlake DW

    更新日期:1986-02-01 00:00:00

  • DNA polymorphism in morels: complete sequences of the internal transcribed spacer of genes coding for rRNA in Morchella esculenta (yellow morel) and Morchella conica (black morel).

    abstract::The internal transcribed spacer (ITS) of the gene coding for rRNA was sequenced in both directions with the gene walking technique in a black morel (Morchella conica) and a yellow morel (M. esculenta) to elucidate the ITS length discrepancy between the two species groups (750-bp ITS in black morels and 1,150-bp ITS in...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.9.3541-3543.1996

    authors: Wipf D,Munch JC,Botton B,Buscot F

    更新日期:1996-09-01 00:00:00

  • Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida.

    abstract:UNLABELLED:In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple-null mut...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01724-16

    authors: Huertas-Rosales Ó,Ramos-González MI,Espinosa-Urgel M

    更新日期:2016-08-30 00:00:00

  • Effects of Nutrient Deprivation on Vibrio cholerae.

    abstract::[This corrects the article on p. 931 in vol. 46.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.5.1193-1193.1984

    authors:

    更新日期:1984-05-01 00:00:00

  • The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway.

    abstract::We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Mic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.3.916-923.1997

    authors: Boundy-Mills KL,de Souza ML,Mandelbaum RT,Wackett LP,Sadowsky MJ

    更新日期:1997-03-01 00:00:00

  • Characterization of a Dehalobacter coculture that dechlorinates 1,2-dichloroethane to ethene and identification of the putative reductive dehalogenase gene.

    abstract::Dehalobacter and "Dehalococcoides" spp. were previously shown to be involved in the biotransformation of 1,1,2-trichloroethane (1,1,2-TCA) and 1,2-dichloroethane (1,2-DCA) to ethene in a mixed anaerobic enrichment culture. Here we report the further enrichment and characterization of a Dehalobacter sp. from this mixed...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02037-08

    authors: Grostern A,Edwards EA

    更新日期:2009-05-01 00:00:00

  • Chemical and Metabolic Controls on Dihydroxyacetone Metabolism Lead to Suboptimal Growth of Escherichia coli.

    abstract::In this work, we shed light on the metabolism of dihydroxyacetone (DHA), a versatile, ubiquitous, and important intermediate for various chemicals in industry, by analyzing its metabolism at the system level in Escherichia coli Using constraint-based modeling, we show that the growth of E. coli on DHA is suboptimal an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00768-19

    authors: Peiro C,Millard P,de Simone A,Cahoreau E,Peyriga L,Enjalbert B,Heux S

    更新日期:2019-07-18 00:00:00

  • Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633.

    abstract::An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity. The highly l-enantioselective enzyme resembled thiol reagent-sensitive alkaline serine proteinases and was strongly activated by divalent cations. It possessed a h...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.12.4330-4334.1993

    authors: Hermes HF,Sonke T,Peters PJ,van Balken JA,Kamphuis J,Dijkhuizen L,Meijer EM

    更新日期:1993-12-01 00:00:00

  • A model for the combined effects of temperature and salt concentration on growth rate of food spoilage molds.

    abstract::We modeled mold growth on a solid culture medium at various temperatures and NaCl concentrations by using five common food spoilage molds (Penicillium roqueforti, Trichoderma harzianum, Paecilomyces variotii, Aspergillus niger, and Emericella nidulans). For the description of the growth rate (expressed as the increase...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.10.3764-3769.1997

    authors: Cuppers HG,Oomes S,Brul S

    更新日期:1997-10-01 00:00:00

  • Production of ochratoxin A in barley by Aspergillus ochraceus and Penicillium viridicatum: effect of fungal growth, time, temperature, and inoculum size.

    abstract::Moistened barley was inoculated with 1.4 x 10(3) and 1.4 x 10(5) spores, respectively, from ochratoxin A-producing strains of Aspergillus ochraceus and Penicillium varidicatum. To estimate fungal tissue in the barley, the amount of glucosamine was followed for 28 days at 10 and 25 degrees C. Ochratoxin A was also foll...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.5.1205-1207.1982

    authors: Häggblom P

    更新日期:1982-05-01 00:00:00

  • Enumeration of potentially pathogenic bacteria from sewage sludges.

    abstract::To ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. A processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.1.118-126.1980

    authors: Dudley DJ,Guentzel MN,Ibarra MJ,Moore BE,Sagik BP

    更新日期:1980-01-01 00:00:00

  • Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins.

    abstract::To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.5.1378-1385.1990

    authors: Van Rie J,Jansens S,Höfte H,Degheele D,Van Mellaert H

    更新日期:1990-05-01 00:00:00

  • Diffusion of macromolecules in model oral biofilms.

    abstract::The diffusive penetration of fluorescently tagged macromolecular solutes into model oral biofilms was visualized by time-lapse microscopy. All of the solutes tested, including dextrans, proteases, green fluorescent protein, and immunoglobulin G, accessed the interior of cell clusters 100 to 200 microm in diameter with...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02279-08

    authors: Takenaka S,Pitts B,Trivedi HM,Stewart PS

    更新日期:2009-03-01 00:00:00

  • Bacterial fouling in a model core system.

    abstract::We have used a sintered glass bead core to simulate the spaces and surfaces of reservoir rock in studies of the bacterial plugging phenomenon that affects waterflood oil recovery operations. The passage of pure or mixed natural populations of bacteria through this solid matrix was initially seen to promote the formati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.3.693-701.1985

    authors: Shaw JC,Bramhill B,Wardlaw NC,Costerton JW

    更新日期:1985-03-01 00:00:00

  • Trichothecene mycotoxins in aerosolized conidia of Stachybotrys atra.

    abstract::Stachybotrys atra is the etiologic agent of stachybotryotoxicosis, and this fungus and its trichothecene mycotoxins were recently implicated in an outbreak of unexplained illness in homes. S. atra was grown on sterile rice, autoclaved, dried, and then aerosolized by acoustic vibration. The distribution of particles (m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.6.1370-1375.1987

    authors: Sorenson WG,Frazer DG,Jarvis BB,Simpson J,Robinson VA

    更新日期:1987-06-01 00:00:00

  • Utilization of dissolved nitrogen by heterotrophic bacterioplankton: effect of substrate c/n ratio.

    abstract::The significance of dissolved combined amino acids (DCAA), dissolved free amino acids (DFAA), and dissolved DNA (D-DNA) as sources of C and N for marine bacteria in batch cultures with variable substrate C/N ratios was studied. Glucose, ammonium, alanine, and phosphate were added to the cultures to produce C/N ratios ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.11.4124-4133.1994

    authors: Jørgensen NO,Kroer N,Coffin RB

    更新日期:1994-11-01 00:00:00

  • Role of chemotaxis in the ecology of denitrifiers.

    abstract::A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO(3) and NO(2) by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.1.109-114.1985

    authors: Kennedy MJ,Lawless JG

    更新日期:1985-01-01 00:00:00

  • Susceptibility of a field-derived, Bacillus thuringiensis-resistant strain of diamondback moth to in vitro-activated Cry1Ac toxin.

    abstract::Resistant and susceptible populations of the diamondback moth (Plutella xylostella) were tested with crystalline, solubilized, and partially and fully activated forms of the Bacillus thuringiensis Cry1Ac delta-endotoxin. Fully activated toxin greatly reduced the resistance ratio (ratio of the 50% lethal concentration ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.67.9.4372-4373.2001

    authors: Sayyed AH,Gatsi R,Kouskoura T,Wright DJ,Crickmore N

    更新日期:2001-09-01 00:00:00

  • Carbohydrate metabolism in mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis.

    abstract::ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00397-08

    authors: Suzuki E,Ohkawa H,Moriya K,Matsubara T,Nagaike Y,Iwasaki I,Fujiwara S,Tsuzuki M,Nakamura Y

    更新日期:2010-05-01 00:00:00

  • Deletion of creB in Aspergillus oryzae increases secreted hydrolytic enzyme activity.

    abstract::Aspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. Targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01406-13

    authors: Hunter AJ,Morris TA,Jin B,Saint CP,Kelly JM

    更新日期:2013-09-01 00:00:00

  • Effect of solar radiation and predacious microorganisms on survival of fecal and other bacteria.

    abstract::The effect of solar radiation and predacious microorganisms on the survival of bacteria of fecal and plant origin was studied. The decline in the numbers of Escherichia coli cells in estuarine water samples was found to be significantly greater in the presence of both naturally occurring microbial predators and solar ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.5.1083-1087.1981

    authors: McCambridge J,McMeekin TA

    更新日期:1981-05-01 00:00:00

  • Production of lycopene in the non-carotenoid-producing yeast Yarrowia lipolytica.

    abstract::The codon-optimized genes crtB and crtI of Pantoea ananatis were expressed in Yarrowia lipolytica under the control of the TEF1 promoter of Y. lipolytica. Additionally, the rate-limiting genes for isoprenoid biosynthesis in Y. lipolytica, GGS1 and HMG1, were overexpressed to increase the production of lycopene. All of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03167-13

    authors: Matthäus F,Ketelhot M,Gatter M,Barth G

    更新日期:2014-03-01 00:00:00

  • Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale.

    abstract::Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00335-09

    authors: Lauber CL,Hamady M,Knight R,Fierer N

    更新日期:2009-08-01 00:00:00