Rubber-degrading enzyme from a bacterial culture.

abstract：:To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene ...

journal_title：Applied and environmental microbiology

authors： Zhang C,Rausch D,Zhang W

更新日期：2009-10-01 00:00:00

abstract：:The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrog...

journal_title：Applied and environmental microbiology

authors： Cotta SR,Dias AC,Marriel IE,Andreote FD,Seldin L,van Elsas JD

更新日期：2014-10-01 00:00:00

abstract：:Methanogenic isolates did not consume hydrogen below partial pressures of 6.5 Pa. Thus, in contrast to a previous report, results from pure-culture studies do not invalidate the threshold model for methane production from hydrogen in sediments. ...

journal_title：Applied and environmental microbiology

authors： Lovley DR

更新日期：1985-06-01 00:00:00

abstract：:A full-scale study evaluating an inoculum addition to stimulate in situ bioremediation of oily-sludge-contaminated soil was conducted at an oil refinery where the indigenous population of hydrocarbon-degrading bacteria in the soil was very low (10(3) to 10(4) CFU/g of soil). A feasibility study was conducted prior to ...

journal_title：Applied and environmental microbiology

authors： Mishra S,Jyot J,Kuhad RC,Lal B

更新日期：2001-04-01 00:00:00

abstract：:This work demonstrates the ability of a bacterial concentration and recovery procedure combined with three different PCR assays targeting the lacZ, wecG, and 16S rRNA genes, respectively, to detect the presence of total coliforms in 100-ml samples of potable water (presence/absence test). PCR assays were first compare...

journal_title：Applied and environmental microbiology

authors： Maheux AF,Boudreau DK,Bisson MA,Dion-Dupont V,Bouchard S,Nkuranga M,Bergeron MG,Rodriguez MJ

更新日期：2014-07-01 00:00:00

abstract：:Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described. ...

journal_title：Applied and environmental microbiology

authors： Allcock ER,Reid SJ,Jones DT,Woods DR

更新日期：1982-03-01 00:00:00

abstract：:Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only c...

journal_title：Applied and environmental microbiology

authors： Morales SE,Holben WE

更新日期：2009-05-01 00:00:00

abstract：:It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences...

journal_title：Applied and environmental microbiology

authors： Liu Z,Ma P,Holtsmark I,Skaugen M,Eijsink VG,Brurberg MB

更新日期：2013-09-01 00:00:00

abstract：:Several transposable elements were isolated from the genome of Xanthomonas oryzae pv. oryzae. These elements and an avirulence gene isolated from X. oryzae pv. oryzae were used as hybridization probes for a collection of X. oryzae pv. oryzae strains from the Philippines. Each of the sequences was present in multiple c...

journal_title：Applied and environmental microbiology

authors： Nelson RJ,Baraoidan MR,Cruz CM,Yap IV,Leach JE,Mew TW,Leung H

更新日期：1994-09-01 00:00:00

abstract：:Predicting the presence of enteric viruses in surface waters is a complex modeling problem. Multiple water quality parameters that indicate the presence of human fecal material, the load of fecal material, and the amount of time fecal material has been in the environment are needed. This paper presents the results of ...

journal_title：Applied and environmental microbiology

authors： Black LE,Brion GM,Freitas SJ

更新日期：2007-06-01 00:00:00

abstract：:The plant-pathogenic fungus Fusarium oxysporum was successfully transformed with the beta-D-glucuronidase gene from Escherichia coli (gusA) (GUS system) in combination with the gene for nitrate reductase (niaD) as the selectable marker. The frequency of cotransformation, as determined by GUS expression on plates conta...

journal_title：Applied and environmental microbiology

authors： Couteaudier Y,Daboussi MJ,Eparvier A,Langin T,Orcival J

更新日期：1993-06-01 00:00:00

abstract：:A greenhouse experiment was conducted to determine the influence of two tropical isolates of Glomus fasciculatum and Glomus mosseae on the nutrient uptake and growth of Sesbania grandiflora. Inoculation of sterile soil with the fungi significantly improved growth and nutrient uptake by S. grandiflora, but the response...

journal_title：Applied and environmental microbiology

authors： Habte M,Aziz T

更新日期：1985-09-01 00:00:00

abstract：:The impact of host nutrition on symbiont regulation in the pea aphid Acyrthosiphon pisum was investigated. The population density of the obligate symbiont Buchnera aphidicola positively correlated with dietary nitrogen levels. In contrast, the population density of the facultative symbiont Serratia symbiotica increase...

journal_title：Applied and environmental microbiology

authors： Wilkinson TL,Koga R,Fukatsu T

更新日期：2007-02-01 00:00:00

abstract：:In various sediments in which Fe(III) reduction was the terminal electron-accepting process, [C]glucose was fermented to C-fatty acids in a manner similar to that observed in methanogenic sediments. These results are consistent with the hypothesis that, in Fe(III)-reducing sediments, fermentable substrates are oxidize...

journal_title：Applied and environmental microbiology

authors： Lovley DR,Phillips EJ

更新日期：1989-12-01 00:00:00

abstract：:The Lactobacillus johnsonii VPI 11088 groESL operon was localized on the chromosome near the insertion element IS1223. The operon was initially cloned as a series of three overlapping PCR fragments, which were sequenced and used to design primers to amplify the entire operon. The amplified fragment was used as a probe...

journal_title：Applied and environmental microbiology

authors： Walker DC,Girgis HS,Klaenhammer TR

更新日期：1999-07-01 00:00:00

abstract：:Biofilms were grown from wild-type (WT) Pseudomonas aeruginosa PAO1 and the cell signaling lasI mutant PAO1-JP1 under laminar and turbulent flows to investigate the relative contributions of hydrodynamics and cell signaling for biofilm formation. Various biofilm morphological parameters were quantified using Image Str...

journal_title：Applied and environmental microbiology

authors： Purevdorj B,Costerton JW,Stoodley P

更新日期：2002-09-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:The initial microbial colonization of tooth surfaces is a repeatable and selective process, with certain bacterial species predominating in the nascent biofilm. Characterization of the initial microflora is the first step in understanding interactions among community members that shape ensuing biofilm development. Usi...

journal_title：Applied and environmental microbiology

authors： Diaz PI,Chalmers NI,Rickard AH,Kong C,Milburn CL,Palmer RJ Jr,Kolenbrander PE

更新日期：2006-04-01 00:00:00

abstract：:Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25 degrees C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number o...

journal_title：Applied and environmental microbiology

authors： Sanders SW,Maxcy RB

更新日期：1979-09-01 00:00:00

abstract：:Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid conta...

journal_title：Applied and environmental microbiology

authors： Ahn WS,Park SJ,Lee SY

更新日期：2000-08-01 00:00:00

abstract：:Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of...

journal_title：Applied and environmental microbiology

authors： Iwamoto S,Motomura K,Shinoda Y,Urata M,Kato J,Takiguchi N,Ohtake H,Hirota R,Kuroda A

更新日期：2007-09-01 00:00:00

abstract：:Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

journal_title：Applied and environmental microbiology

authors： Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

更新日期：2012-07-01 00:00:00

abstract：:An inexpensive, simple-to-construct nephelometer which was used to monitor the lysis of spheroplasts is described. The nephelometer is a flow-through device with a linear response to cell concentration from the lower detection limit to 8 x 10(8) cells per ml. ...

journal_title：Applied and environmental microbiology

authors： Smith TL,Witter LD

更新日期：1982-12-01 00:00:00

abstract：:A new genus and species of a nonmotile gram-negative rod, Syntrophobacter wolinii, is the first bacterium described which degrades propionate only in coculture with an H(2)-using organism and in the absence of light or exogenous electron acceptors such as O(2), sulfate, or nitrate. It was isolated from methanogenic en...

journal_title：Applied and environmental microbiology

authors： Boone DR,Bryant MP

更新日期：1980-09-01 00:00:00

abstract：:The use of DNA probes for Shiga-like toxin I (SLT-I) and SLT-II for detection of SLT-producing Escherichia coli (SLTEC) in foods and calf fecal samples was evaluated. Enrichment cultures were prepared from food or fecal samples. Colonies formed by plating of enrichment cultures were probed for SLTEC by colony hybridiz...

journal_title：Applied and environmental microbiology

authors： Samadpour M,Liston J,Ongerth JE,Tarr PI

更新日期：1990-05-01 00:00:00

abstract：:The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

journal_title：Applied and environmental microbiology

authors： Babich H,Borenfreund E

更新日期：1991-07-01 00:00:00

abstract：:Phragmidium violaceum causes leaf rust on the European blackberry (Rubus fruticosus L. aggregate). Multiple strains of this pathogen have been introduced into southern Australia for the biological control of at least 15 taxa of European blackberry, a nonindigenous, invasive plant. In climates conducive to leaf rust, t...

journal_title：Applied and environmental microbiology

authors： Gomez DR,Evans KJ,Baker J,Harvey PR,Scott ES

更新日期：2008-09-01 00:00:00

abstract：:Bacterial aggregates from a chemolithoautotrophic, nitrifying fluidized bed reactor were investigated with microsensors and rRNA-based molecular techniques. The microprofiles of O2, NH4+, NO2-, and NO3- demonstrated the occurrence of complete nitrification in the outer 125 microgram of the aggregates. The ammonia oxid...

journal_title：Applied and environmental microbiology

authors： Schramm A,De Beer D,Wagner M,Amann R

更新日期：1998-09-01 00:00:00

abstract：:To achieve functional bioluminescence, the developing light organ of newly hatched juveniles of the Hawaiian squid Euprymna scolopes must become colonized by luminous, symbiosis-competent Vibrio fischeri present in the ambient seawater. This benign infection occurs rapidly in animals placed in seawater from the host's...

journal_title：Applied and environmental microbiology

authors： Lee K,Ruby EG

更新日期：1995-01-01 00:00:00

abstract：:Citrate and pyruvate metabolism by nongrowing cells of Leuconostoc oenos was investigated. (sup13)C nuclear magnetic resonance (NMR) spectroscopy was used to elucidate the pathway of citrate breakdown and to probe citrate or pyruvate utilization, noninvasively, in living cell suspensions. The utilization of isotopical...

journal_title：Applied and environmental microbiology

authors： Ramos A,Lolkema JS,Konings WN,Santos H

更新日期：1995-04-01 00:00:00