Inexpensive laboratory-constructed nephelometer.

abstract：:The virulence factor internalin A (InlA) facilitates the uptake of Listeria monocytogenes by epithelial cells that express the human isoform of E-cadherin. Previous studies identified naturally occurring premature stop codon (PMSC) mutations in inlA and demonstrated that these mutations are responsible for virulence a...

journal_title：Applied and environmental microbiology

authors： Van Stelten A,Nightingale KK

更新日期：2008-12-01 00:00:00

abstract：:Differences in individual host responses have emerged as an issue regarding the health benefits of probiotics. Here, we applied ribosome engineering (RE) technology, developed in an actinomycete study, to Lacticaseibacillus rhamnosus GG (LGG). RE can effectively enhance microbial potential by using antibiotics to indu...

journal_title：Applied and environmental microbiology

authors： Ishida M,Namai F,Shigemori S,Kajikawa S,Tsukagoshi M,Sato T,Ogita T,Shimosato T

更新日期：2020-10-01 00:00:00

abstract：:This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data ...

journal_title：Applied and environmental microbiology

authors： Awerbuch TE,Stark AA

更新日期：1979-12-01 00:00:00

abstract：:The growth of marine bacteria under iron-limited conditions was investigated. Neither siderophore production nor bacterial growth was detected for Pelagiobacter sp. strain V0110 when Fe(III) was present in the culture medium at a concentration of <1.0 microM. However, the growth of V0110 was strongly stimulated by the...

journal_title：Applied and environmental microbiology

authors： Guan LL,Onuki H,Kamino K

更新日期：2000-07-01 00:00:00

abstract：:Thiobacillus ferrooxidans is an acidophilic organism important to metal leaching of low-grade ores. The aforementioned importance is related to the ability of the bacterium to oxidize reduced iron and sulfur, principally found in nature as pyrite (FeS2). The present study dealt with sulfide oxidation at low pH values ...

journal_title：Applied and environmental microbiology

authors： Tano T,Lundgren D

更新日期：1978-06-01 00:00:00

abstract：:Because dihydrostreptomycin can remain as a slowly removed antibiotic residue in dairy animals and because of the need for more sensitive procedures with which to provide information concerning antibiotic residues in food products, procedures were developed in more sensitive assays of dihydrostreptomycin in milk and s...

journal_title：Applied and environmental microbiology

authors： Inglis JM,Katz SE

更新日期：1978-03-01 00:00:00

abstract：:Relationships between the hyphae of Agaricus bisporus (Lang) Sing and bacteria from the mushroom bed casing layer were examined with a scanning electron microscope. Hyphae growing in the casing layer differed morphologically from compost-grown hyphae. Whereas the compost contained thin single hyphae surrounded by calc...

journal_title：Applied and environmental microbiology

authors： Masaphy S,Levanon D,Tchelet R,Henis Y

更新日期：1987-05-01 00:00:00

abstract：:The gene encoding a novel penicillin G acylase (PGA), designated pgaW, was cloned from Achromobacter xylosoxidans and overexpressed in Escherichia coli. The pgaW gene contains an open reading frame of 2586 nucleotides. The deduced protein sequence encoded by pgaW has about 50% amino acid identity to several well-chara...

journal_title：Applied and environmental microbiology

authors： Cai G,Zhu S,Yang S,Zhao G,Jiang W

更新日期：2004-05-01 00:00:00

abstract：:In a field study of 29 dairy farms, Pseudomonas aeruginosa was isolated more frequently (P = 0.05) from milking parlor udder wash water systems containing iodophor germicides than from those with no germicide. Most available iodine (AI2) concentrations were below the recommended level of 25 ppm (25 microgram/ml). Rubb...

journal_title：Applied and environmental microbiology

authors： Hicks CR,Eberhart RJ,Erskine RJ,Spencer SB

更新日期：1991-02-01 00:00:00

abstract：:Trichoderma species isolated from water-damaged buildings were screened for toxicity by using boar sperm cells as indicator cells. The crude methanolic cell extract from Trichoderma harzianum strain ES39 inhibited the boar sperm cell motility at a low exposure concentration (50% effective concentration, 1 to 5 microg ...

journal_title：Applied and environmental microbiology

authors： Peltola J,Ritieni A,Mikkola R,Grigoriev PA,Pócsfalvi G,Andersson MA,Salkinoja-Salonen MS

更新日期：2004-08-01 00:00:00

abstract：:A "repair-detection" procedure consisting of pour plating of food samples with Trypticase soy agar, followed by 1-h repair incubation at room temperature and subsequent overlay with violet red bile agar, was found to be an effective method for the detection of injuried and uninjuried coliforms from dairy products. Thi...

journal_title：Applied and environmental microbiology

authors： Ray B,Speck ML

更新日期：1978-04-01 00:00:00

abstract：:During microaerophilic growth of magnetic spirillum MS-1 on tartrate and nitrate, a maximal cell density was obtained at an initial oxygen partial pressure of 17 Pa. A transient accumulation of nitrous oxide and a 1:2 (mol/mol) stoichiometry between tartrate oxidation and nitrate reduction were observed, indicating th...

journal_title：Applied and environmental microbiology

authors： Escalante-Semerena JC,Blakemore RP,Wolfe RS

更新日期：1980-08-01 00:00:00

abstract：:Isolates of Gaeumannomyces graminis var. tritici, the causal agent of take-all of wheat, varied in sensitivity in vitro to the antibiotics phenazine-1-carboxylic acid (PCA) and 2,4-diacetylphloroglucinol (Phl) produced by fluorescent Pseudomonas spp. shown previously to have potential for biological control of this pa...

journal_title：Applied and environmental microbiology

authors： Mazzola M,Fujimoto DK,Thomashow LS,Cook RJ

更新日期：1995-07-01 00:00:00

abstract：:A gene coding for an aminopeptidase (PepC) from Lactococcus lactis subsp. cremoris AM2 was cloned by complementation of an Escherichia coli mutant lacking aminopeptidase activity. The nucleotide sequence was determined. A portion of the predicted amino acid sequence of PepC (436 amino acids) showed strong homology to ...

journal_title：Applied and environmental microbiology

authors： Chapot-Chartier MP,Nardi M,Chopin MC,Chopin A,Gripon JC

更新日期：1993-01-01 00:00:00

abstract：:Determination of microbial viability by the plate count method is routine in microbiology laboratories worldwide. However, limitations of the technique, particularly with respect to environmental microorganisms, are widely recognized. Many alternatives based upon viability staining have been proposed, and these are of...

journal_title：Applied and environmental microbiology

authors： Davey HM

更新日期：2011-08-15 00:00:00

abstract：:We developed a system to monitor the transfer of heterologous DNA from a genetically manipulated strain of Saccharomyces cerevisiae to Escherichia coli. This system is based on a yeast strain that carries multiple integrated copies of a pUC-derived plasmid. The bacterial sequences are maintained in the yeast genome by...

journal_title：Applied and environmental microbiology

authors： Adam AC,González-Blasco G,Rubio-Texeira M,Polaina J

更新日期：1999-12-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：:The present study aimed at the long-term storage of rumen protozoa as living cells in liquid nitrogen. The two-step or interrupted slow freezing procedure was used to cryopreserve six of the dominant species of rumen ciliates isolated from monofaunated animals, Dasytricha ruminantium, Entodinium caudatum, Epidinium ec...

journal_title：Applied and environmental microbiology

authors： Nsabimana E,Kisidayová S,Macheboeuf D,Newbold CJ,Jouany JP

更新日期：2003-07-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00

abstract：:Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 1...

journal_title：Applied and environmental microbiology

authors： Humrighouse BW,Adcock NJ,Rice EW

更新日期：2011-09-01 00:00:00

abstract：:The potential of Pseudomonas pseudoflava to produce poly-beta-hydroxyalkanoates (PHAs) from pentoses was studied. This organism was able to use a hydrolysate from the hemicellulosic fraction of poplar wood as a carbon and energy source for its growth. However, in batch cultures, growth was inhibited completely at hydr...

journal_title：Applied and environmental microbiology

authors： Bertrand JL,Ramsay BA,Ramsay JA,Chavarie C

更新日期：1990-10-01 00:00:00

abstract：:Plant polysaccharide breakdown by microbes in the rumen is fundamental to digestion in ruminant livestock. Bacterial species belonging to the rumen genera Butyrivibrio and Pseudobutyrivibrio are important degraders and utilizers of lignocellulosic plant material. These bacteria degrade polysaccharides and ferment the ...

journal_title：Applied and environmental microbiology

authors： Palevich N,Kelly WJ,Leahy SC,Denman S,Altermann E,Rakonjac J,Attwood GT

更新日期：2019-12-13 00:00:00

abstract：:The interaction of the peptide antibiotic nisin with liposomes has been studied. The effect of this interaction was analyzed on the membrane potential (inside negative) and the pH gradient (inside alkaline) in liposomes made from Escherichia coli phosphatidylethanolamine and egg phosphatidylcholine (9:1, wt/wt). The m...

journal_title：Applied and environmental microbiology

authors： Gao FH,Abee T,Konings WN

更新日期：1991-08-01 00:00:00

abstract：:DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...

journal_title：Applied and environmental microbiology

authors： Huys G,D'Haene K,Van Eldere J,von Holy A,Swings J

更新日期：2005-01-01 00:00:00

abstract：:Candida parapsilosis, C. tropicalis, and Torulopsis glabrata were the human-associated yeasts most frequently isolated from quahogs, oysters, and mussels collected from four estuarine areas along the northern shore of Long Island Sound. Some inconsistency and seasonal variation in the occurrence of these and other yea...

journal_title：Applied and environmental microbiology

authors： Buck JD,Bubucis PM,Combs TJ

更新日期：1977-02-01 00:00:00

abstract：:Lactase (beta-d-galactosidase) was produced by Candida pseudotropicalis grown in deproteinized whey. Maximum enzyme production in 2% whey was obtained by supplementation with 0.15% yeast extract, 0.1% (NH(4))(2)SO(4), and 0.05% KH(2)PO(4) (wt/vol). Highest enzyme values (4.35 U/mg of cells and 68 U/ml) were obtained w...

journal_title：Applied and environmental microbiology

authors： de Bales SA,Castillo FJ

更新日期：1979-06-01 00:00:00

abstract：:Vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related Vibrio mimicus. The two species share many genes coding for proteins, such as ctxAB, and show almost identical 16S DNA coding for rRNA (rDNA) sequences. Primers targeting conserved...

journal_title：Applied and environmental microbiology

authors： Chun J,Huq A,Colwell RR

更新日期：1999-05-01 00:00:00

abstract：:We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testing 136 hot-water-system samples collected from 55 sites as well as 49 cooling tower samples collected from 20 different sites, in parallel with the standard culture method. The PCR assay was reproducible and suitable for...

journal_title：Applied and environmental microbiology

authors： Yaradou DF,Hallier-Soulier S,Moreau S,Poty F,Hillion Y,Reyrolle M,André J,Festoc G,Delabre K,Vandenesch F,Etienne J,Jarraud S

更新日期：2007-03-01 00:00:00

abstract：:Bacillus sp. strain SS, an aerobic, gram-positive sporeformer, was isolated from seeds of Astragalus crotalariae, a selenium-accumulating plant. This bacillus grew in a nutrient broth (containing beef extract and peptone) if the medium was supplemented with high concentrations of selenium. Concentrations of Na(2)SeO(3...

journal_title：Applied and environmental microbiology

authors： Lindblow-Kull C,Shrift A,Gherna RL

更新日期：1982-09-01 00:00:00

abstract：:Sakacin A is an antilisterial bacteriocin produced by Lactobacillus sake Lb706. In order to identify genes involved in sakacin A production and immunity, the plasmid fraction of L. sake Lb706 was shotgun cloned directly into a sakacin A-nonproducing and -sensitive variant, L. sake Lb706-B, by using the broad-host-rang...

journal_title：Applied and environmental microbiology

authors： Axelsson L,Holck A,Birkeland SE,Aukrust T,Blom H

更新日期：1993-09-01 00:00:00