Abstract:
:Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestion profiles identical to that of the chocolate milk isolate.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Proctor ME,Brosch R,Mellen JW,Garrett LA,Kaspar CW,Luchansky JBdoi
10.1128/AEM.61.8.3177-3179.1995subject
Has Abstractpub_date
1995-08-01 00:00:00pages
3177-9issue
8eissn
0099-2240issn
1098-5336journal_volume
61pub_type
杂志文章abstract::The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01777-06
更新日期:2006-12-01 00:00:00
abstract::Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.10.3972-3977.1997
更新日期:1997-10-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH) and rRNA slot blot hybridization with 16S rRNA-targeted oligonucleotide probes were used to investigate the phylogenetic composition of a marine Arctic sediment (Svalbard). FISH resulted in the detection of a large fraction of microbes living in the top 5 cm of the sediment. U...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.1.387-395.2001
更新日期:2001-01-01 00:00:00
abstract::In a field study of 29 dairy farms, Pseudomonas aeruginosa was isolated more frequently (P = 0.05) from milking parlor udder wash water systems containing iodophor germicides than from those with no germicide. Most available iodine (AI2) concentrations were below the recommended level of 25 ppm (25 microgram/ml). Rubb...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.2.568-572.1991
更新日期:1991-02-01 00:00:00
abstract::A field evaluation of double violet agar for the isolation and presumptive identification of Klebsiella pneumoniae from water has been performed. Water from the North Oconee River, Clarke County, Ga., was cultured for presence of klebsiellae using the membrane filter technique. Colonies were presumptively identified a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.31.2.213-215.1976
更新日期:1976-02-01 00:00:00
abstract::The contamination of waterways with fecal material is a persistent threat to public health. Identification of the sources of fecal contamination is a vital component for abatement strategies and for determination of total maximum daily loads. While phenotypic and genotypic techniques have been used to determine potent...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02764-05
更新日期:2006-06-01 00:00:00
abstract::Heterocystous filamentous cyanobacterium Anabaena cylindrica B629 and nonheterocystous filamentous cyanobacterium Oscillatoria sp. strain Miami BG7 were cultured in media with N(2) as the sole nitrogen source; and activities of oxygen-dependent hydrogen uptake, photohydrogen production, photooxygen evolution, and resp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.2.287-291.1985
更新日期:1985-08-01 00:00:00
abstract::Growth of Lactococcus lactis subsp. lactis biovar diacetylactis was observed on media with citrate as the only energy source. At pH 5.6, steady state was achieved in a chemostat on a citrate-containing medium in the absence of a carbohydrate. Under these conditions, pyruvate, acetate, and some acetoin and butanediol w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.12.4216-4222.1993
更新日期:1993-12-01 00:00:00
abstract::Bacteria can survive on hospital textiles and surfaces, from which they can be disseminated, representing a source of health care-associated infections (HCAIs). Surfaces containing copper (Cu), which is known for its bactericidal properties, could be an efficient way to lower the burden of potential pathogens. The ant...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02266-12
更新日期:2012-12-01 00:00:00
abstract::Infectious salmon anemia (ISA) is a serious disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), belonging to the genus Isavirus, family Orthomyxoviridae. There is an urgent need to understand the virulence factors and pathogenic mechanisms of ISAV and to develop new vaccine approaches. U...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03153-14
更新日期:2015-02-01 00:00:00
abstract::Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.8.2551-2558.1990
更新日期:1990-08-01 00:00:00
abstract::A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.8.3053-3056.1996
更新日期:1996-08-01 00:00:00
abstract::Gonometa podocarpi is an important pest of several species of pine in East Africa, and large numbers of trees in plantations in Kenya were partially or completely defoliated by the larval stage of this insect. After the infestation in the Mt. Elgon region, large numbers of dead and moribund larvae were found on the gr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.42.2.308-316.1981
更新日期:1981-08-01 00:00:00
abstract::The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.5.1667-1673.1997
更新日期:1997-05-01 00:00:00
abstract::Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.2971-2977.1992
更新日期:1992-09-01 00:00:00
abstract::In the luminal contents of metronidazole-treated rats, there was a dominant Bifidobacterium species. A strain has been isolated, its 16S rRNA gene has been sequenced, and the strain has been named Bifidobacterium pseudolongum strain Patronus. In this study, using an experimental model of healthy rats, the effects of m...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01731-08
更新日期:2009-01-01 00:00:00
abstract::Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04028-12
更新日期:2013-05-01 00:00:00
abstract::For efficient generation of high-affinity protein-based binding molecules, fast and reliable downstream characterization platforms are needed. In this work, we have explored the use of staphylococcal cell surface display together with flow cytometry for affinity characterization of candidate affibody molecules directl...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01432-07
更新日期:2007-11-01 00:00:00
abstract::Microcystis aeruginosa is a common cyanobacterium in water blooms that appear widely in nutrient-rich, fresh, and brackish waters, and its toxic blooms cause the death of domestic animals. The administration of a crude toxic cell extract of M. aeruginosa K-139 to mice can produce tumor necrosis factor (TNF) and prompt...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.1.327-330.1991
更新日期:1991-01-01 00:00:00
abstract::To study the anaerobic degradation of the chimera 3-chloro-4-hydroxybenzoate (3-Cl,4-OHB), anaerobic freshwater sediment samples from the vicinity of Athens, Ga., were adapted for the transformation of 4-hydroxybenzoate (4-OHB), 3-chlorobenzoate (3-CB), 2-chlorophenol (2-CP), and 2,4-dichlorophenol (2,4-DCP). In nonad...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.11.3580-3585.1992
更新日期:1992-11-01 00:00:00
abstract::Bacterioplankton numbers, biomasses, and productivities, as well as chlorophyll a concentrations and phytoplankton productivities, were assayed from 1 March 1984 to 12 August 1985 through a 250-m-deep seawater column in Howe Sound, a temperate fjord-sound on the southern coast of British Columbia, Canada. Primary prod...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.6.1277-1285.1987
更新日期:1987-06-01 00:00:00
abstract::An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated in this study. This rapid method was compared to the standard method followed in the United Kingdom. We used 1,011 samples originating from 20...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.10.4401-4407.2000
更新日期:2000-10-01 00:00:00
abstract::Toxin-producing isolates of Bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. The toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.10.4637-4645.1999
更新日期:1999-10-01 00:00:00
abstract::The heat destruction characteristics of Clostridium botulinum spores suspended in tomato juice and phosphate buffer were determined by the survivor curve method with aluminum thermal death time tubes. Two type A strains of C. botulinum and a type B strain were evaluated. Strains A16037 and B15580 were implicated in ou...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.34.1.23-29.1977
更新日期:1977-07-01 00:00:00
abstract::Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soil...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.2.1008-1010.2001
更新日期:2001-02-01 00:00:00
abstract::A trans unsaturated fatty acid was found as a major constituent in the lipids of Pseudomonas putida P8. The fatty acid was identified as 9-trans-hexadecenoic acid by gas chromatography, argentation thin-layer chromatography, and infrared absorption spectrometry. Growing cells of P. putida P8 reacted to the presence of...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.6.1847-1852.1992
更新日期:1992-06-01 00:00:00
abstract::Thirty-two chemoheterotrophic bacteria were isolated from unsaturated subsurface soil samples obtained from ca. 70 m below land surface in a high desert in southeastern Idaho. Most isolates were gram positive (84%) and strict aerobes (79%). Acridine orange direct counts of microbes in one subsurface sample showed lowe...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.9.2420-2423.1989
更新日期:1989-09-01 00:00:00
abstract::Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other ty...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.9.5119-5131.2004
更新日期:2004-09-01 00:00:00
abstract::Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of al...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.12.5514-5517.2000
更新日期:2000-12-01 00:00:00
abstract::Eight strains of the entomopathogen Bacillus popilliae were analyzed by pulsed-field gel electrophoresis, and genomic size estimates of ;sim2,600 to 3,500 kb were obtained. The type strain, ATCC 14706, had a genomic size of 3,395 kb. For the six New Zealand isolates, the degree of similarity in the pulsed-field gel el...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.6.2446-2449.1995
更新日期:1995-06-01 00:00:00