Glucose-stat, a glucose-controlled continuous culture.

Abstract:

:A predictive and feedback proportional control algorithm, developed for fed-batch fermentations and described in a companion paper (G. L. Kleman, J. J. Chalmers, G. W. Luli, and W. R. Strohl, Appl. Environ. Microbiol. 57:910-917, 1991), was used in this work to control a continuous culture on the basis of the soluble-glucose concentration (called the glucose-stat). This glucose-controlled continuous-culture system was found to reach and maintain steady state for 11 to 24 residence times when four different background glucose concentrations (0.27, 0.50, 0.7, and 1.5 g/liter) were used. The predictive-plus-feedback control system yielded very tight control of the continuous nutristat cultures; glucose concentrations were maintained at the set points with less than 0.003 standard error. Acetate production by Escherichia coli B in glucose-stats was found not to be correlated with the level of steady-state soluble-glucose concentration.

journal_name

Appl Environ Microbiol

authors

Kleman GL,Chalmers JJ,Luli GW,Strohl WR

doi

10.1128/AEM.57.4.918-923.1991

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

918-23

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

57

pub_type

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