Construction and expression of sugar kinase transcriptional gene fusions by using the Sinorhizobium meliloti ORFeome.

abstract：:The LlaDII restriction/modification (R/M) system was found on the naturally occurring 8.9-kb plasmid pHW393 in Lactococcus lactis subsp. cremoris W39. A 2.4-kb PstI-EcoRI fragment inserted into the Escherichia coli-L. lactis shuttle vector pCI3340 conferred to L. lactis LM2301 and L. lactis SMQ86 resistance against re...

journal_title：Applied and environmental microbiology

authors： Madsen A,Josephsen J

更新日期：1998-07-01 00:00:00

abstract：:In this work, we report the expression and secretion of the leaderless two-peptide (EntL50A and EntL50B) bacteriocin enterocin L50 from Enterococcus faecium L50 by the methylotrophic yeast Pichia pastoris X-33. The bacteriocin structural genes entL50A and entL50B were fused to the Saccharomyces cerevisiae gene region ...

journal_title：Applied and environmental microbiology

authors： Basanta A,Gómez-Sala B,Sánchez J,Diep DB,Herranz C,Hernández PE,Cintas LM

更新日期：2010-05-01 00:00:00

abstract：:A six-step biochemical key is presented for the identification of all recognized Enterococcus spp. The key consists of 12 tests, but no more than 6 are needed for the most complicated identification. The reliability of the key has been evaluated with collection type strains and clinical and environmental isolates. Thi...

journal_title：Applied and environmental microbiology

authors： Manero A,Blanch AR

更新日期：1999-10-01 00:00:00

abstract：:DNA primers corresponding to conserved motifs in bacterial repetitive (REP, ERIC, and BOX) elements and PCR were used to show that REP-, ERIC-, and BOX-like DNA sequences are widely distributed in phytopathogenic Xanthomonas and Pseudomonas strains. REP-, ERIC, and BOX-PCR (collectively known as rep-PCR) were used to ...

journal_title：Applied and environmental microbiology

authors： Louws FJ,Fulbright DW,Stephens CT,de Bruijn FJ

更新日期：1994-07-01 00:00:00

abstract：:Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

journal_title：Applied and environmental microbiology

authors： Gandhi KM,Mandrell RE,Tian P

更新日期：2010-12-01 00:00:00

abstract：:In a medium containing ammonia, proteose peptone, and cysteine as nitrogen sources, 17 of 24 Bacteroidaceae strains, 3 of Selenomonas strains, 1 of 7 curved rods, 3 of 7 Spirochaetaceae strains, 8 of 20 Eubacterium strains, 8 of 13 Peptococcaceae strains, 3 of 4 Clostridium strains, 19 of 20 Enterobacteriaceae strains...

journal_title：Applied and environmental microbiology

authors： Takahashi M,Benno Y,Mitsuoka T

更新日期：1980-01-01 00:00:00

abstract：:Mycophenolic acid (MPA) is the active ingredient in the increasingly important immunosuppressive pharmaceuticals CellCept (Roche) and Myfortic (Novartis). Despite the long history of MPA, the molecular basis for its biosynthesis has remained enigmatic. Here we report the discovery of a polyketide synthase (PKS), MpaC,...

journal_title：Applied and environmental microbiology

authors： Regueira TB,Kildegaard KR,Hansen BG,Mortensen UH,Hertweck C,Nielsen J

更新日期：2011-05-01 00:00:00

abstract：:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) ...

journal_title：Applied and environmental microbiology

authors： Villarreal DM,Phillips CL,Kelley AM,Villarreal S,Villaloboz A,Hernandez P,Olson JS,Henderson DP

更新日期：2008-09-01 00:00:00

abstract：:Seasonal variation in the phylogenetic composition of Synechococcus assemblages in estuarine and coastal waters of Hong Kong was examined through pyrosequencing of the rpoC1 gene. Sixteen samples were collected in 2009 from two stations representing estuarine and ocean-influenced coastal waters, respectively. Synechoc...

journal_title：Applied and environmental microbiology

authors： Xia X,Vidyarathna NK,Palenik B,Lee P,Liu H

更新日期：2015-11-01 00:00:00

abstract：:A method was developed to study the biodegradation and oxidative biodehalogenation of fluorinated phenols by 19F nuclear magnetic resonance (NMR). Characterization of the 19F NMR spectra of metabolite profiles of a series of fluorophenols, converted by purified phenol hydroxylase, catechol 1,2-dioxygenase, and/or by t...

journal_title：Applied and environmental microbiology

authors： Boersma MG,Dinarieva TY,Middelhoven WJ,van Berkel WJ,Doran J,Vervoort J,Rietjens IM

更新日期：1998-04-01 00:00:00

abstract：:Dibenzo-p-dioxin was oxidatively cleaved by the dibenzofuran-degrading bacterium Pseudomonas sp. strain HH69 to produce minor amounts of 1-hydroxydibenzo-p-dioxin and catechol, while a 2-phenoxy derivative of muconic acid was formed as the major product. Upon acidic methylation, the latter yielded the dimethylester of...

journal_title：Applied and environmental microbiology

authors： Harms H,Wittich RM,Sinnwell V,Meyer H,Fortnagel P,Francke W

更新日期：1990-04-01 00:00:00

abstract：:Many recombinant proteins that are produced in Escherichia coli have to be targeted to the periplasm to be functional. N-terminal signal peptides can be used to direct recombinant proteins to the membrane-embedded Sec translocon, a multiprotein complex that translocates proteins across the membrane into the periplasm....

journal_title：Applied and environmental microbiology

authors： Ytterberg AJ,Zubarev RA,Baumgarten T

更新日期：2019-06-17 00:00:00

abstract：:To ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. A processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers o...

journal_title：Applied and environmental microbiology

authors： Dudley DJ,Guentzel MN,Ibarra MJ,Moore BE,Sagik BP

更新日期：1980-01-01 00:00:00

abstract：:Concerns regarding the depletion of the world's reserves of oil and global climate change have promoted an intensification of research and development toward the production of biofuels and other alternative sources of energy during the last years. There is currently much interest in developing the technology for third...

journal_title：Applied and environmental microbiology

authors： Ortiz-Marquez JC,Do Nascimento M,Dublan Mde L,Curatti L

更新日期：2012-04-01 00:00:00

abstract：:The ability of two closely related species to maintain species boundaries in spite of retained interfertility between them is a documented driving force of speciation. Experimental evidence to support possible interspecific postzygotic isolation mechanisms for organisms belonging to the kingdom Fungi is still missing....

journal_title：Applied and environmental microbiology

authors： Garbelotto M,Gonthier P,Nicolotti G

更新日期：2007-10-01 00:00:00

abstract：:The induction of the inducible lysyl-tRNA synthetase, LysU, and the inducible lysine and arginine decarboxylases of Escherichia coli K-12 grown in AC broth to a pH of 5.5 or less is temperature dependent, being distinctly lower at 24 than at 37 degrees C. This induction does not appear to be under HtpR control. ...

journal_title：Applied and environmental microbiology

authors： Hassani M,Pincus DH,Bennett GN,Hirshfield IN

更新日期：1992-08-01 00:00:00

abstract：:A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...

journal_title：Applied and environmental microbiology

authors： Delécluse A,Rosso ML,Ragni A

更新日期：1995-12-01 00:00:00

abstract：:During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...

journal_title：Applied and environmental microbiology

authors： Nielsen JC,Richelieu M

更新日期：1999-02-01 00:00:00

abstract：:Lactobacillus gasseri LA39 and L. reuteri LA6 isolated from feces of the same human infant were found to produce similar cyclic bacteriocins (named gassericin A and reutericin 6, respectively) that cannot be distinguished by molecular weights or primary amino acid sequences. However, reutericin 6 has a narrower spectr...

journal_title：Applied and environmental microbiology

authors： Kawai Y,Ishii Y,Arakawa K,Uemura K,Saitoh B,Nishimura J,Kitazawa H,Yamazaki Y,Tateno Y,Itoh T,Saito T

更新日期：2004-05-01 00:00:00

abstract：:We sought to determine whether microorganisms from the polychlorinated biphenyl (PCB)-contaminated sediment in Woods Pond (Lenox, Mass.) could dehalogenate brominated biphenyls. The PCB dechlorination specificities for the microorganisms in this sediment have been well characterized. This allowed us to compare the deh...

journal_title：Applied and environmental microbiology

authors： Bedard DL,Van Dort HM

更新日期：1998-03-01 00:00:00

abstract：:Western Lake Erie (Laurentian Great Lakes) is prone to annual cyanobacterial harmful algal blooms (cHABs) dominated by Microcystis spp. that often yield microcystin toxin concentrations exceeding the federal EPA recreational contact advisory of 8 μg liter-1 In August 2014, microcystin levels were detected in finished ...

journal_title：Applied and environmental microbiology

authors： McKindles KM,Manes MA,DeMarco JR,McClure A,McKay RM,Davis TW,Bullerjahn GS

更新日期：2020-10-28 00:00:00

abstract：:Various Listeria monocytogenes strains may contaminate a single food product, potentially resulting in simultaneous exposure of consumers to multiple strains. However, due to bias in strain recovery, L. monocytogenes strains isolated from foods by selective enrichment (SE) might not always represent those that can bet...

journal_title：Applied and environmental microbiology

authors： Zilelidou E,Karmiri CV,Zoumpopoulou G,Mavrogonatou E,Kletsas D,Tsakalidou E,Papadimitriou K,Drosinos E,Skandamis P

更新日期：2016-12-01 00:00:00

abstract：:We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture. ...

journal_title：Applied and environmental microbiology

authors： Hirata Y,Minami J,Koyama M,Matsushita O,Katayama S,Jin F,Maeta H,Okabe A

更新日期：1995-11-01 00:00:00

abstract：:To determine the potential for bacteriophage-mediated gene transfer in the marine environment, we established transduction systems by using marine phage host isolates. Plasmid pQSR50, which contains transposon Tn5 and encodes kanamycin and streptomycin resistance, was used in plasmid transduction assays. Both marine b...

journal_title：Applied and environmental microbiology

authors： Jiang SC,Paul JH

更新日期：1998-08-01 00:00:00

abstract：:A "repair-detection" procedure consisting of pour plating of food samples with Trypticase soy agar, followed by 1-h repair incubation at room temperature and subsequent overlay with violet red bile agar, was found to be an effective method for the detection of injuried and uninjuried coliforms from dairy products. Thi...

journal_title：Applied and environmental microbiology

authors： Ray B,Speck ML

更新日期：1978-04-01 00:00:00

abstract：:A novel thermostable alcohol dehydrogenase (ADH) showing activity toward aromatic secondary alcohols was identified from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (TkADH). The gene, tk0845, which encodes an aldo-keto reductase, was heterologously expressed in Escherichia coli. The enzyme was found ...

journal_title：Applied and environmental microbiology

authors： Wu X,Zhang C,Orita I,Imanaka T,Fukui T,Xing XH

更新日期：2013-04-01 00:00:00

abstract：:The bacterial host ranges of the conjugatively self-transmissible IncN group plasmid pCU1 and its mobilizable miniderivative, pCU785, were examined. Species of the gram-negative purple bacteria were chosen for this study. Conjugative mobilization of pCU785 into a wide variety of bacteria was facilitated by the presenc...

journal_title：Applied and environmental microbiology

authors： Krishnan BR,Iyer VN

更新日期：1988-09-01 00:00:00

abstract：:Fungal multicopper oxidases have many potential industrial applications, since they perform reactions under mild conditions. We isolated a phenol oxidase from the fungus Acremonium murorum var. murorum that was capable of decolorizing plant chromophores (such as anthocyanins). This enzyme is of interest in laundry-cle...

journal_title：Applied and environmental microbiology

authors： Gouka RJ,van der Heiden M,Swarthoff T,Verrips CT

更新日期：2001-06-01 00:00:00

abstract：:Microorganisms in diverse terrestrial surface and subsurface environments can anaerobically catalyze the oxidative dissolution of uraninite. While a limited quantity (∼5 to 12 μmol liter(-1)) of uranium is oxidatively dissolved in pure culture studies, the metabolism is coupled to electron transport, providing the pot...

journal_title：Applied and environmental microbiology

authors： Weber KA,Thrash JC,Van Trump JI,Achenbach LA,Coates JD

更新日期：2011-07-01 00:00:00

abstract：:Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of ...

journal_title：Applied and environmental microbiology

authors： Inaba T,Mirocha CJ

更新日期：1979-01-01 00:00:00