Expression of rhizobial nitrogenase: influence of plant cell-conditioned medium.

abstract：:To enhance the production of isoprene, a volatile 5-carbon hydrocarbon, in the Gram-positive spore-forming rod-shaped bacterium Bacillus subtilis, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and 1-deoxy-d-xylulose-5-phosphate reductoisomerase (Dxr) were overexpressed in B. subtilis DSM 10. For the strain that overex...

journal_title：Applied and environmental microbiology

authors： Xue J,Ahring BK

更新日期：2011-04-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) viruses in some fungi are associated with hypovirulence and have been used or proposed as biological control agents. We isolated 7.5-kb dsRNAs from 13 of 286 field strains of Fusarium graminearum isolated from maize in Korea. One of these strains, DK21, was examined in more detail. This str...

journal_title：Applied and environmental microbiology

authors： Chu YM,Jeon JJ,Yea SJ,Kim YH,Yun SH,Lee YW,Kim KH

更新日期：2002-05-01 00:00:00

abstract：:The erythromycin resistance gene ermB has been found in a variety of gram-positive bacteria. This gene has also been found in Bacteroides species but only in six recently isolated strains; thus, the gene seems to have entered this genus only recently. One of the six Bacteroides ermB-containing isolates, WH207, could t...

journal_title：Applied and environmental microbiology

authors： Gupta A,Vlamakis H,Shoemaker N,Salyers AA

更新日期：2003-11-01 00:00:00

abstract：:We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonel...

journal_title：Applied and environmental microbiology

authors： Liu J,Xie Z,Merritt J,Qi F

更新日期：2012-05-01 00:00:00

abstract：:Bacterioplankton numbers, biomasses, and productivities, as well as chlorophyll a concentrations and phytoplankton productivities, were assayed from 1 March 1984 to 12 August 1985 through a 250-m-deep seawater column in Howe Sound, a temperate fjord-sound on the southern coast of British Columbia, Canada. Primary prod...

journal_title：Applied and environmental microbiology

authors： Albright LJ,McCrae SK

更新日期：1987-06-01 00:00:00

abstract：:Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days,...

journal_title：Applied and environmental microbiology

authors： Bérubé A,Trudel M,Payment P

更新日期：1989-06-01 00:00:00

abstract：:High concentrations of free metal ions in the environment can be detrimental to bacterial survival. However, bacteria utilize strategies, including the activation of stress response pathways and immobilizing chemical elements on their surface, to limit this toxicity. In this study, we characterized LA4131, the HtpX-li...

journal_title：Applied and environmental microbiology

authors： Henry R,Lo M,Khoo C,Zhang H,Boysen RI,Picardeau M,Murray GL,Bulach DM,Adler B

更新日期：2013-08-01 00:00:00

abstract：:Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 microg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-micros duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatment...

journal_title：Applied and environmental microbiology

authors： Pol IE,Mastwijk HC,Bartels PV,Smid EJ

更新日期：2000-01-01 00:00:00

abstract：:Chrysophanol and islandicin, two anthraquinones which are structurally related to emodin, were found to be frame-shift mutagens for Salmonella typhimurium strain TA 1537 after metabolic activation. ...

journal_title：Applied and environmental microbiology

authors： Liberman DF,Schaefer FL,Fink RC,Ramgopal M,Ghosh AC,Mulcahy R

更新日期：1980-09-01 00:00:00

abstract：:Characteristics of an obligately methylotrophic coccoid methanogen (strain GS-16) previously isolated from estuarine sediment are described. Growth was demonstrated on dimethyl sulfide (DMS) or trimethylamine (TMA), but not on methane thiol, methane thiol plus hydrogen, dimethyl disulfide, or methionine. DMS-grown cel...

journal_title：Applied and environmental microbiology

authors： Oremland RS,Kiene RP,Mathrani I,Whiticar MJ,Boone DR

更新日期：1989-04-01 00:00:00

abstract：:Pleurotus ostreatus is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO allev...

journal_title：Applied and environmental microbiology

authors： Hou L,Zhao M,Huang C,Wu X,Zhang J

更新日期：2020-02-18 00:00:00

abstract：:Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...

journal_title：Applied and environmental microbiology

authors： Bender J,Vatcharapijarn Y,Jeffries TW

更新日期：1985-03-01 00:00:00

abstract：:To examine the relationship between activated-sludge bulking and levels of specific filamentous bacteria, we developed a statistics-based quantification method for estimating the biomass levels of specific filaments using 16S rRNA-targeted fluorescent in situ hybridization (FISH) probes. The results of quantitative FI...

journal_title：Applied and environmental microbiology

authors： Liao J,Lou I,de los Reyes FL 3rd

更新日期：2004-04-01 00:00:00

abstract：:The thermoacidophile Acidianus is widely distributed in Yellowstone National Park hot springs that span large gradients in pH (1.60 to 4.84), temperature (42 to 90°C), and mineralogical composition. To characterize the potential role of flexibility in mineral-dependent energy metabolism in contributing to the widespre...

journal_title：Applied and environmental microbiology

authors： Amenabar MJ,Boyd ES

更新日期：2018-05-31 00:00:00

abstract：:Vibrio cholerae O1 in plankton samples collected from ponds and rivers between February 1987 and January 1990 in Matlab, Bangladesh, was detected by the fluorescent-monoclonal antibody (FA) technique. Samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites that were part of a...

journal_title：Applied and environmental microbiology

authors： Huq A,Colwell RR,Rahman R,Ali A,Chowdhury MA,Parveen S,Sack DA,Russek-Cohen E

更新日期：1990-08-01 00:00:00

abstract：:The high risk associated with biological threat agents dictates that any suspicious sample be handled under strict surety and safety controls and processed under high-level containment in specialized laboratories. This study attempted to find a rapid, reliable, and simple method for the complete inactivation of a wide...

journal_title：Applied and environmental microbiology

authors： Sagripanti JL,Hülseweh B,Grote G,Voss L,Böhling K,Marschall HJ

更新日期：2011-10-01 00:00:00

abstract：:The incidence of human infection with Campylobacter jejuni is increasing in most developed countries and the reason for this is largely unknown. Although poultry meat is considered to be a major source, it is evident that other reservoirs exist, possibly common to humans and poultry. Environmental sources are believed...

journal_title：Applied and environmental microbiology

authors： Petersen L,Nielsen EM,Engberg J,On SL,Dietz HH

更新日期：2001-07-01 00:00:00

abstract：:Aquaglyceroporins (GlpFs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. Fungal species form a large group of eukaryotic organisms, and their GlpFs may be diverse, exhibiting various activities. However, few filamentous fungal GlpFs have been biol...

journal_title：Applied and environmental microbiology

authors： Liu XD,Wei Y,Zhou XY,Pei X,Zhang SH

更新日期：2015-10-01 00:00:00

abstract：:Previous work has shown that Trametes (Coriolus) versicolor bleaches kraft pulp brownstock with the concomitant release of methanol. In this work, the fungus is shown to produce both laccase and manganese peroxidase (MnP) but not lignin peroxidase during pulp bleaching. MnP production was enhanced by the presence of p...

journal_title：Applied and environmental microbiology

authors： Paice MG,Reid ID,Bourbonnais R,Archibald FS,Jurasek L

更新日期：1993-01-01 00:00:00

abstract：:The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...

journal_title：Applied and environmental microbiology

authors： Kadokura H,Yoda K,Imai M,Yamasaki M

更新日期：1990-09-01 00:00:00

abstract：:The methanotrophic bacterium Methylosinus trichosporium OB3b is unusually active in degrading recalcitrant haloalkanes such as trichloroethylene (TCE). The first and rate-limiting step in the degradation of TCE is catalyzed by a soluble methane monooxygenase (sMMO). This enzyme is not expressed when the cells are grow...

journal_title：Applied and environmental microbiology

authors： Phelps PA,Agarwal SK,Speitel GE,Georgiou G

更新日期：1992-11-01 00:00:00

abstract：:The variation in manure-amended soil survival capability among 18 Escherichia coli O157 strains (8 animal, 1 food, and 9 human isolates) was studied using a single sandy soil sample and a single sample of cattle manure as the inoculum carrier. The virulence profiles of E. coli O157 strains were characterized by detect...

journal_title：Applied and environmental microbiology

authors： Franz E,van Hoek AH,Bouw E,Aarts HJ

更新日期：2011-11-01 00:00:00

abstract：:Strain SR, a monensin-sensitive, ammonia-producing ruminal bacterium, grew rapidly on arginine and lysine, but only if sodium was present. Arginine transport could be driven by either an electrical potential or a chemical gradient of sodium. Arginine was converted to ornithine, and it appeared that ornithine efflux cr...

journal_title：Applied and environmental microbiology

authors： Van Kessel JS,Russell JB

更新日期：1992-03-01 00:00:00

abstract：:We examined the genetic structure and symbiotic characteristics of Bradyrhizobium isolates recovered from four legume species (Lupinus albus [white lupine], Lupinus angustifolius [blue lupine], Ornithopus compressus [yellow serradella], and Macroptilium atropurpureum [sirato]) grown in an Oregon soil. We established t...

journal_title：Applied and environmental microbiology

authors： Bottomley PJ,Cheng HH,Strain SR

更新日期：1994-06-01 00:00:00

abstract：:The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange. ...

journal_title：Applied and environmental microbiology

authors： Johansson HE,Johansson MK,Wong AC,Armstrong ES,Peterson EJ,Grant RE,Roy MA,Reddington MV,Cook RM

更新日期：2011-06-01 00:00:00

abstract：:The presence and chain length of mycolic acids of bacteria of the genera Corynebacterium, Rhodococcus, Gordona, Mycobacterium, and Arthrobacter and of coryneform bacteria containing a type B peptidoglycan were related to the cell surface hydrophobicity of the bacteria, which in turn was related to adhesion of the cell...

journal_title：Applied and environmental microbiology

authors： Bendinger B,Rijnaarts HH,Altendorf K,Zehnder AJ

更新日期：1993-11-01 00:00:00

abstract：:The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and MonoQ-Sepharose. T...

journal_title：Applied and environmental microbiology

authors： Wanker E,Huber A,Schwab H

更新日期：1995-05-01 00:00:00

abstract：:Many Helicobacter pylori genetic studies would benefit from an ability to move DNA sequences easily between strains by transformation and homologous recombination, without needing to leave a conventional drug resistance determinant at the targeted locus. Presented here is a two-gene cassette that can be selected both ...

journal_title：Applied and environmental microbiology

authors： Dailidiene D,Dailide G,Kersulyte D,Berg DE

更新日期：2006-09-01 00:00:00

abstract：:Pep5 is a cationic pore-forming lantibiotic produced by Staphylococcus epidermidis strain 5. The producer strain protects itself from the lethal action of its own bacteriocin through the 69-amino-acid immunity peptide PepI. The N-terminal segment of PepI contains a 20-amino-acid stretch of apolar residues, whereas the...

journal_title：Applied and environmental microbiology

authors： Hoffmann A,Schneider T,Pag U,Sahl HG

更新日期：2004-06-01 00:00:00

abstract：:A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon in...

journal_title：Applied and environmental microbiology

authors： Bouhenni R,Gehrke A,Saffarini D

更新日期：2005-08-01 00:00:00