Discovery and description of giant submarine smectite cones on the seafloor in Eyjafjordur, northern Iceland, and a novel thermal microbial habitat.

abstract：:Cellulase and xylanase cDNAs were isolated from a cDNA library of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 constructed in Escherichia coli. The cellulase cDNA (celB) was 1.8 kb long with an open reading frame (ORF) coding for a polypeptide of 471 amino acids, and the xylanase cDNA (xynA) was 1.2 kb...

journal_title：Applied and environmental microbiology

authors： Li XL,Chen H,Ljungdahl LG

更新日期：1997-02-01 00:00:00

abstract：:A unique lipolytic bacterium was isolated in a selective growth system consisting of 99% triglycerides and a 1% water phase. The bacterium, termed Pseudomonas aeruginosa YS-7, was able to grow in an environment of low water content and could also survive amphipathic, osmotic, and matrical water stress in a triglycerid...

journal_title：Applied and environmental microbiology

authors： Shabtai Y

更新日期：1991-06-01 00:00:00

abstract：:A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...

journal_title：Applied and environmental microbiology

authors： Clough SJ,Flavier AB,Schell MA,Denny TP

更新日期：1997-03-01 00:00:00

abstract：:Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stabil...

journal_title：Applied and environmental microbiology

authors： Teachey ME,McDonald JM,Ottesen EA

更新日期：2019-05-16 00:00:00

abstract：:A clinical isolate of Pseudomonas aeruginosa was found capable of utilizing salicylate by the salicylate hydroxylase and beta-ketoadipate pathway. ...

journal_title：Applied and environmental microbiology

authors： Ohta S,Matsumoto H,Terawaki Y

更新日期：1981-01-01 00:00:00

abstract：:We investigated the anaerobic biodegradation of 3-chlorobenzoate (3CBz) by microorganisms from an aquifer where chloroaromatic compounds were previously found to resist decay in the presence of sulfate. After a lengthy lag period, 3CBz was degraded in the presence of sulfate and concurrently with sulfate reduction. Ch...

journal_title：Applied and environmental microbiology

authors： Townsend GT,Ramanand K,Suflita JM

更新日期：1997-07-01 00:00:00

abstract：:Isolation of amoebae from the surface and internal tissues of edible mushrooms was investigated. Samples tested over a 3-year period included mushrooms cultivated from six geographic localities. Of 168 mushroom surfaces tested, 161 (96%) yielded amoebae. Of 166 samples of internal stalk and cap tissues tested, only 1 ...

journal_title：Applied and environmental microbiology

authors： Napolitano JJ

更新日期：1982-07-01 00:00:00

abstract：:Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Sidhu JPS,Smith K,Beale DJ,Gyawali P,Toze S

更新日期：2015-12-18 00:00:00

abstract：:Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that s...

journal_title：Applied and environmental microbiology

authors： Trinh CT,Srienc F

更新日期：2009-11-01 00:00:00

abstract：:A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essent...

journal_title：Applied and environmental microbiology

authors： Serrano A,Williams T,Simón O,López-Ferber M,Caballero P,Muñoz D

更新日期：2013-02-01 00:00:00

abstract：:Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of...

journal_title：Applied and environmental microbiology

authors： Klein PG,Juneja VK

更新日期：1997-11-01 00:00:00

abstract：:A purified lipase from the yeast Cryptococcus sp. strain S-2 exhibited remote homology to proteins belonging to the cutinase family rather than to lipases. This enzyme could effectively degrade the high-molecular-weight compound polylactic acid, as well as other biodegradable plastics, including polybutylene succinate...

journal_title：Applied and environmental microbiology

authors： Masaki K,Kamini NR,Ikeda H,Iefuji H

更新日期：2005-11-01 00:00:00

abstract：:We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

更新日期：2005-12-01 00:00:00

abstract：:The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity....

journal_title：Applied and environmental microbiology

authors： Zappe H,Jones WA,Jones DT,Woods DR

更新日期：1988-05-01 00:00:00

abstract：:Legionella pneumophila proliferates in freshwater environments at temperatures ranging from 25 to 45°C. To investigate the preference of different sequence types (ST) for a specific temperature range, growth of L. pneumophila serogroup 1 (SG1) ST1 (environmental strains), ST47, and ST62 (disease-associated strains) wa...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Brouwer-Hanzens AJ,Veenendaal HR,Wullings BA

更新日期：2016-10-27 00:00:00

abstract：:Fluctuations in ciliated protozoan concentrations were monitored in 40 individually fed crossbred heifers that were stepped up to an 85% concentrate diet either slowly (12 days) or rapidly (3 days), with or without monensin (30 ppm). Ruminal fluid was withdrawn from all animals by stomach tube at the start of the stud...

journal_title：Applied and environmental microbiology

authors： Towne G,Nagaraja TG,Brandt RT Jr,Kemp KE

更新日期：1990-10-01 00:00:00

abstract：:A collection of 300 isolates of fluorescent pseudomonads was established from Douglas fir-Laccaria bicolor mycorrhizas and mycorrhizosphere and from adjacent bulk soil. These isolates were first phenotypically characterized with the Biolog method. Taxonomic identification assigned 90% of the isolates to the different ...

journal_title：Applied and environmental microbiology

authors： Frey P,Frey-Klett P,Garbaye J,Berge O,Heulin T

更新日期：1997-05-01 00:00:00

abstract：:A glycosyltransferase, involved in the synthesis of cyclic maltosylmaltose [CMM; cyclo-{-->6)-alpha-D-Glcp(1-->4)-alpha-D-Glcp(1-->6)-alpha-D-Glcp(1-->4)-alpha-D-Glcp(1-->}] from starch, was purified to homogeneity from the culture supernatant of Arthrobacter globiformis M6. The CMM-forming enzyme had a molecular mass...

journal_title：Applied and environmental microbiology

authors： Mukai K,Watanabe H,Kubota M,Chaen H,Fukuda S,Kurimoto M

更新日期：2006-02-01 00:00:00

abstract：:Mn exerted various effects on the growth of Leptothrix discophora strain SS-1 in batch cultures depending on the concentration added to the medium. Concentrations of 0.55 to 5.5 muM Mn, comparable to those in the environment from which strain SS-1 was isolated, decreased cell yield and prolonged stationary-phase survi...

journal_title：Applied and environmental microbiology

authors： Adams LF,Ghiorse WC

更新日期：1985-03-01 00:00:00

abstract：:Enterotoxigenic Escherichia coli (ETEC) strains that produce immunologically heterogeneous fimbriae and enterotoxins are the primary cause of neonatal diarrhea and postweaning diarrhea in young pigs. A multivalent vaccine inducing protective immunity against ideally all ETEC fimbriae and enterotoxins could be effectiv...

journal_title：Applied and environmental microbiology

authors： Lu T,Moxley RA,Zhang W

更新日期：2019-05-16 00:00:00

abstract：:Cholesterol and albumin are limiting factors in the growth of Mycoplasma species. These nutrients are usually supplied in the culture medium by the addition of serum. The growth of M. pneumoniae in a serum-free medium containing an ethanolic cholesterol suspension and albumin was about one-half the level attained in s...

journal_title：Applied and environmental microbiology

authors： Cluss RG,Johnson JK,Somerson NL

更新日期：1983-08-01 00:00:00

abstract：:Iron(III)-reducing bacteria have been demonstrated to rapidly catalyze the reduction and immobilization of uranium(VI) from contaminated subsurface sediments. Thus, these organisms may aid in the development of bioremediation strategies for uranium contamination, which is prevalent in acidic subsurface sediments at U....

journal_title：Applied and environmental microbiology

authors： Petrie L,North NN,Dollhopf SL,Balkwill DL,Kostka JE

更新日期：2003-12-01 00:00:00

abstract：:This study aimed at investigating the antifungal activity of Wickerhamomyces anomalus and sourdough lactic acid bacteria to extend the shelf life of wheat flour bread. The antifungal activity was assayed by agar diffusion, growth rate inhibition, and conidial germination assays, using Penicillium roqueforti DPPMAF1 as...

journal_title：Applied and environmental microbiology

authors： Coda R,Cassone A,Rizzello CG,Nionelli L,Cardinali G,Gobbetti M

更新日期：2011-05-01 00:00:00

abstract：:In this study, multilocus sequence typing (MLST) was combined with the genetic detection of six genetic markers, ansB, dmsA, ggt, cj1585c, cjj81176-1367/71 (cj1365c), and the two-gene marker tlp7 (cj0951c plus cj0952c), to assess if their presence correlated with different C. jejuni clonal groups. Using a collection o...

journal_title：Applied and environmental microbiology

authors： Zautner AE,Herrmann S,Corso J,Tareen AM,Alter T,Gross U

更新日期：2011-04-01 00:00:00

abstract：:Root-colonizing, saprophytic fluorescent pseudomonads of the Pseudomonas putida-P. fluorescens group express similar levels of catalase and superoxide dismutase activities during growth on a sucrose- and amino acid-rich medium. Increased specific activities of catalase but not superoxide dismutase were observed during...

journal_title：Applied and environmental microbiology

authors： Katsuwon J,Anderson AJ

更新日期：1990-11-01 00:00:00

abstract：:The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was clos...

journal_title：Applied and environmental microbiology

authors： Dedysh SN,Pankratov TA,Belova SE,Kulichevskaya IS,Liesack W

更新日期：2006-03-01 00:00:00

abstract：:We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an ...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Lewis DA,Ocampo-Sosa AA,Fogarty U,Makrai L,Navas J,Scortti M,Hernández M,Vázquez-Boland JA

更新日期：2006-06-01 00:00:00

abstract：:The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed t...

journal_title：Applied and environmental microbiology

authors： Lemaître JP,Duroux A,Pimpie R,Duez JM,Milat ML

更新日期：2015-03-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00

abstract：:For simultaneous identification of members of the betaproteobacterial order "Rhodocyclales" in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting of 79 probes was developed. Probe design was based on phylogenetic analysis of available 16S rRNA sequences from all cultu...

journal_title：Applied and environmental microbiology

authors： Loy A,Schulz C,Lücker S,Schöpfer-Wendels A,Stoecker K,Baranyi C,Lehner A,Wagner M

更新日期：2005-03-01 00:00:00