Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants.

Abstract:

:A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute toward the elucidation of the role of such mutants in baculovirus populations.

journal_name

Appl Environ Microbiol

authors

Serrano A,Williams T,Simón O,López-Ferber M,Caballero P,Muñoz D

doi

10.1128/AEM.03021-12

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

1118-25

issue

4

eissn

0099-2240

issn

1098-5336

pii

AEM.03021-12

journal_volume

79

pub_type

杂志文章
  • Function of native OmtA in vivo and expression and distribution of this protein in colonies of Aspergillus parasiticus.

    abstract::The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.11.5718-5727.2002

    authors: Lee LW,Chiou CH,Linz JE

    更新日期:2002-11-01 00:00:00

  • Transformation of mercuric chloride and methylmercury by the rumen microflora.

    abstract::The microflora in strained rumen fluid did not methylate or volatilize 203Hg2+ at detectable rates. However, there was an exponential decay in the concentration of added CH3Hg+, which was attributed to demethylation. The major product of demethylation was metallic mercury (Hg0), and it was released as a volatile produ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.4.626-636.1979

    authors: Kozak S,Forsberg CW

    更新日期:1979-10-01 00:00:00

  • Novel forms of structural integration between microbes and a hydrothermal vent gastropod from the Indian Ocean.

    abstract::Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of gamma-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting diff...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.5.3082-3090.2004

    authors: Goffredi SK,Warén A,Orphan VJ,Van Dover CL,Vrijenhoek RC

    更新日期:2004-05-01 00:00:00

  • Extremely acidophilic protists from acid mine drainage host Rickettsiales-lineage endosymbionts that have intervening sequences in their 16S rRNA genes.

    abstract::During a molecular phylogenetic survey of extremely acidic (pH < 1), metal-rich acid mine drainage habitats in the Richmond Mine at Iron Mountain, Calif., we detected 16S rRNA gene sequences of a novel bacterial group belonging to the order Rickettsiales in the Alphaproteobacteria. The closest known relatives of this ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5512-5518.2003

    authors: Baker BJ,Hugenholtz P,Dawson SC,Banfield JF

    更新日期:2003-09-01 00:00:00

  • Low-temperature decontamination with hydrogen peroxide or chlorine dioxide for space applications.

    abstract::The currently used microbial decontamination method for spacecraft and components uses dry-heat microbial reduction at temperatures of >110°C for extended periods to prevent the contamination of extraplanetary destinations. This process is effective and reproducible, but it is also long and costly and precludes the us...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07948-11

    authors: Pottage T,Macken S,Giri K,Walker JT,Bennett AM

    更新日期:2012-06-01 00:00:00

  • Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN.

    abstract::Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA (PsJN::gusA11) genes w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.4.1685-1693.2005

    authors: Compant S,Reiter B,Sessitsch A,Nowak J,Clément C,Ait Barka E

    更新日期:2005-04-01 00:00:00

  • Low pore connectivity increases bacterial diversity in soil.

    abstract::One of soil microbiology's most intriguing puzzles is how so many different bacterial species can coexist in small volumes of soil when competition theory predicts that less competitive species should decline and eventually disappear. We provide evidence supporting the theory that low pore connectivity caused by low w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03085-09

    authors: Carson JK,Gonzalez-Quiñones V,Murphy DV,Hinz C,Shaw JA,Gleeson DB

    更新日期:2010-06-01 00:00:00

  • Evidence for coexistence of distinct Escherichia coli populations in various aquatic environments and their survival in estuary water.

    abstract::Escherichia coli, a commensal bacterium from the intestinal tracts of humans and vertebrate animals, has been used as one of two bacterial indicators of fecal contamination, along with intestinal enterococci, to monitor the microbiological quality of water. However, water environments are now recognized as a secondary...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00698-13

    authors: Berthe T,Ratajczak M,Clermont O,Denamur E,Petit F

    更新日期:2013-08-01 00:00:00

  • Suitability of quantitative real-time PCR to estimate the biomass of fungal root endophytes.

    abstract::A nested single-copy locus-based quantitative PCR (qPCR) assay and a multicopy locus-based qPCR assay were developed to estimate endophytic biomass of fungal root symbionts belonging to the Phialocephala fortinii sensu lato-Acephala applanata species complex (PAC). Both assays were suitable for estimation of endophyti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00907-10

    authors: Tellenbach C,Grünig CR,Sieber TN

    更新日期:2010-09-01 00:00:00

  • Formation of 4-hydroxy-2,5-dimethyl-3[2H]-furanone by Zygosaccharomyces rouxii: identification of an intermediate.

    abstract::The formation of the important flavor compound 4-hydroxy-2,5-dimethyl-3[2H]-furanone (HDMF; Furaneol) from D-fructose-1,6-bisphosphate by the yeast Zygosaccharomyces rouxii was studied with regard to the identification of intermediates present in the culture medium. Addition of o-phenylenediamine, a trapping reagent f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.3911-3918.2003

    authors: Hauck T,Brühlmann F,Schwab W

    更新日期:2003-07-01 00:00:00

  • Endosymbiotic microbiota of the bamboo pseudococcid Antonina crawii (Insecta, Homoptera).

    abstract::We characterized the intracellular symbiotic microbiota of the bamboo pseudococcid Antonina crawii by performing a molecular phylogenetic analysis in combination with in situ hybridization. Almost the entire length of the bacterial 16S rRNA gene was amplified and cloned from A. crawii whole DNA. Restriction fragment l...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.2.643-650.2000

    authors: Fukatsu T,Nikoh N

    更新日期:2000-02-01 00:00:00

  • Degradation of 3-chlorobenzoate under low-oxygen conditions in pure and mixed cultures of the anoxygenic photoheterotroph Rhodopseudomonas palustris DCP3 and an aerobic Alcaligenes species.

    abstract::The presence or absence of molecular oxygen has been shown to play a crucial role in the degradability of haloaromatic compounds. In the present study, it was shown that anaerobic phototrophic 3-chlorobenzoate (3CBA) metabolism by Rhodopseudomonas palustris DCP3 is oxygen tolerant up to a concentration of 3 microM O2....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.1.131-137.1999

    authors: Krooneman J,van den Akker S,Pedro Gomes TM,Forney LJ,Gottschal JC

    更新日期:1999-01-01 00:00:00

  • Mathermycin, a Lantibiotic from the Marine Actinomycete Marinactinospora thermotolerans SCSIO 00652.

    abstract::Lantibiotics are antimicrobial peptides belonging to the family of ribosomally synthesized and posttranslationally modified peptides (RiPPs) and feature thioether linkages in their structures. In this study, we identified the biosynthetic gene cluster of a cinnamycin analog, named mathermycin, from Marinactinospora th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00926-17

    authors: Chen E,Chen Q,Chen S,Xu B,Ju J,Wang H

    更新日期:2017-07-17 00:00:00

  • Evaluation of data transformations and validation of a model for the effect of temperature on bacterial growth.

    abstract::The temperature of chilled foods is an important variable for controlling microbial growth in a production and distribution chain. Therefore, it is essential to model growth as a function of temperature in order to predict the number of organisms as a function of temperature and time. This article deals with the corre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.1.195-203.1994

    authors: Zwietering MH,Cuppers HG,de Wit JC,van 't Riet K

    更新日期:1994-01-01 00:00:00

  • Nonlogarithmic death rate calculations for Byssochlamys fulva and other microorganisms.

    abstract::Survivor curves for heat-resistant ascospores of Byssochlamys fulva exposed to lethal heat were nonlogarithmic. At lower heating temperatures, the log survivor curves were characterized by a shoulder plus an accelerating death rate; with increased temperatures, the rate approached logarithmic death. The formula (log N...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.3.596-600.1979

    authors: King AD Jr,Bayne HG,Alderton G

    更新日期:1979-03-01 00:00:00

  • Global regulation of the response to sulfur availability in the cheese-related bacterium Brevibacterium aurantiacum.

    abstract::In this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum. In agreement with the growth of B. aurantiacum in the presence of sulfate and c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01708-10

    authors: Forquin MP,Hébert A,Roux A,Aubert J,Proux C,Heilier JF,Landaud S,Junot C,Bonnarme P,Martin-Verstraete I

    更新日期:2011-02-01 00:00:00

  • Induction of Shiga Toxin-Encoding Prophage by Abiotic Environmental Stress in Food.

    abstract::The prophage-encoded Shiga toxin is a major virulence factor in Stx-producing Escherichia coli (STEC). Toxin production and phage production are linked and occur after induction of the RecA-dependent SOS response. However, food-related stress and Stx-prophage induction have not been studied at the single-cell level. T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01378-17

    authors: Fang Y,Mercer RG,McMullen LM,Gänzle MG

    更新日期:2017-09-15 00:00:00

  • Manipulating the Expression of Small Secreted Protein 1 (Ssp1) Alters Patterns of Development and Metabolism in the White-Rot Fungus Pleurotus ostreatus.

    abstract::The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom Pleurotus ostreatus produces considerable amounts of SSPs at the onset of secondary metabolism, during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00761-19

    authors: Feldman D,Amedi N,Carmeli S,Yarden O,Hadar Y

    更新日期:2019-07-18 00:00:00

  • Role of the arginine deiminase system in protecting oral bacteria and an enzymatic basis for acid tolerance.

    abstract::The arginine deiminase system was found to function in protecting bacterial cells against the damaging effects of acid environments. For example, as little as 2.9 mM arginine added to acidified suspensions of Streptococcus sanguis at a pH of 4.0 resulted in ammonia production and protection against killing. The argini...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1318-1324.1988

    authors: Casiano-Colón A,Marquis RE

    更新日期:1988-06-01 00:00:00

  • Spectrum of the reductive dehalogenation activity of desulfitobacterium frappieri PCP-1

    abstract::Desulfitobacterium frappieri PCP-1 was induced for ortho- and para-dechlorinating activities by different chlorophenols. Dehalogenation rates ranging from 25 to 1,158 nmol/min/mg of cell protein were observed according to the chlorophenol tested and the position of the chlorine removed. D. frappieri shows a broad subs...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.11.4603-4606.1998

    authors: Dennie D,Gladu I I,Lepine F,Villemur R,Bisaillon J,Beaudet R

    更新日期:1998-11-01 00:00:00

  • Brown pigments produced by Yarrowia lipolytica result from extracellular accumulation of homogentisic acid.

    abstract::Yarrowia lipolytica produces brown extracellular pigments that correlate with tyrosine catabolism. During tyrosine depletion, the yeast accumulated homogentisic acid, p-hydroxyphenylethanol, and p-hydroxyphenylacetic acid in the medium. Homogentisic acid accumulated under all aeration conditions tested, but its concen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3463-3468.2001

    authors: Carreira A,Ferreira LM,Loureiro V

    更新日期:2001-08-01 00:00:00

  • Diffusion of macromolecules in model oral biofilms.

    abstract::The diffusive penetration of fluorescently tagged macromolecular solutes into model oral biofilms was visualized by time-lapse microscopy. All of the solutes tested, including dextrans, proteases, green fluorescent protein, and immunoglobulin G, accessed the interior of cell clusters 100 to 200 microm in diameter with...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02279-08

    authors: Takenaka S,Pitts B,Trivedi HM,Stewart PS

    更新日期:2009-03-01 00:00:00

  • Effects of pH on distribution of Listeria ribotypes in corn, hay, and grass silage.

    abstract::Listeria app, isolated from 13 of 129 (10%) corn silage samples, 21 of 76 (28%) hay silage samples, and 3 of 5 (60%) grass silage samples during a previous Vermont survey were subjected to automated ribotype (RT) analysis. The 13 positive corn silage samples contained 3 Listeria monocytogenes isolated (three RTs, incl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.9.3695-3697.1997

    authors: Ryser ET,Arimi SM,Donnelly CW

    更新日期:1997-09-01 00:00:00

  • Influence of alternative electron acceptors on the anaerobic biodegradability of chlorinated phenols and benzoic acids.

    abstract::Nitrate, sulfate, and carbonate were used as electron acceptors to examine the anaerobic biodegradability of chlorinated aromatic compounds in estuarine and freshwater sediments. The respective denitrifying, sulfidogenic, and methanogenic enrichment cultures were established on each of the monochlorinated phenol and m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.4.1162-1167.1993

    authors: Häggblom MM,Rivera MD,Young LY

    更新日期:1993-04-01 00:00:00

  • Growth of Pseudomonas aeruginosa in tap water in relation to utilization of substrates at concentrations of a few micrograms per liter.

    abstract::Five Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.5.1086-1095.1982

    authors: van der Kooij D,Oranje JP,Hijnen WA

    更新日期:1982-11-01 00:00:00

  • Model organisms retain an "ecological memory" of complex ecologically relevant environmental variation.

    abstract::Although tractable model organisms are essential to characterize the molecular mechanisms of evolution and adaptation, the ecological relevance of their behavior is not always clear because certain traits are easily lost during long-term laboratory culturing. Here, we demonstrate that despite their long tenure in the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03280-13

    authors: Beer KD,Wurtmann EJ,Pinel N,Baliga NS

    更新日期:2014-03-01 00:00:00

  • Prevalence and characterization of non-O157 Shiga toxin-producing Escherichia coli on carcasses in commercial beef cattle processing plants.

    abstract::Beef carcass sponge samples collected from July to August 1999 at four large processing plants in the United States were surveyed for the presence of non-O157 Shiga toxin-producing Escherichia coli (STEC). Twenty-eight (93%) of 30 single-source lots surveyed included at least one sample containing non-O157 STEC. Of 33...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.10.4847-4852.2002

    authors: Arthur TM,Barkocy-Gallagher GA,Rivera-Betancourt M,Koohmaraie M

    更新日期:2002-10-01 00:00:00

  • Metabolism of trimethylamine, choline, and glycine betaine by sulfate-reducing and methanogenic bacteria in marine sediments.

    abstract::The response of methanogenesis and sulfate reduction to trimethylamine, choline, and glycine betaine was examined in surface sediments from the intertidal region of Lowes Cove, Maine. Addition of these substrates markedly stimulated methanogenesis in the presence of active sulfate reduction, whereas addition of other ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.4.719-725.1984

    authors: King GM

    更新日期:1984-10-01 00:00:00

  • Changes in Resident Bacteria, pH, Sucrose, and Invert Sugar Levels in Sugarbeet Roots During Storage.

    abstract::Stored sugarbeet roots began fermenting within 24 h after oxygen was depleted at 26 C when the resident bacterial populations increased dramatically. Most of the bacteria present after anaerobic storage for 7 days at 26 C could hydrolyze sucrose in vitro. Although pH and sucrose levels decreased and invert sugar level...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.5.754-757.1976

    authors: Cole DF,Bugbee WM

    更新日期:1976-05-01 00:00:00

  • Improved specimen coating technique for scanning electron microscope observation of decomposer microorganisms.

    abstract::Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.2.313-315.1976

    authors: Draggan S

    更新日期:1976-02-01 00:00:00