Isolation of a single-stranded plasmid from Clostridium acetobutylicum NCIB 6444.

abstract：:The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with d...

journal_title：Applied and environmental microbiology

authors： Germida JJ

更新日期：1988-01-01 00:00:00

abstract：:An aminopeptidase was purified from cell extracts of Lactococcus lactis subsp. cremoris AM2 by ion-exchange chromatography. After electrophoresis of the purified enzyme in the presence or absence of sodium dodecyl sulfate, one protein band was detected. The enzyme was a 300-kilodalton hexamer composed of identical sub...

journal_title：Applied and environmental microbiology

authors： Neviani E,Boquien CY,Monnet V,Thanh LP,Gripon JC

更新日期：1989-09-01 00:00:00

abstract：:The use of Lactococcus lactis (the most extensively characterized lactic acid bacterium) as a delivery organism for heterologous proteins is, in some cases, limited by low production levels and poor-quality products due to surface proteolysis. In this study, we combined in one L. lactis strain use of the nisin-inducib...

journal_title：Applied and environmental microbiology

authors： Miyoshi A,Poquet I,Azevedo V,Commissaire J,Bermudez-Humaran L,Domakova E,Le Loir Y,Oliveira SC,Gruss A,Langella P

更新日期：2002-06-01 00:00:00

abstract：:Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two co...

journal_title：Applied and environmental microbiology

authors： Berk SG,Ting RS,Turner GW,Ashburn RJ

更新日期：1998-01-01 00:00:00

abstract：:Thiosulfate dehydrogenase is known to play a significant role in thiosulfate oxidation in the acidophilic, obligately chemolithoautotroph, Acidithiobacillus ferrooxidans. Enzyme activity measured using ferricyanide as the electron acceptor was detected in cell extracts of A. ferrooxidans ATCC 23270 grown on tetrathion...

journal_title：Applied and environmental microbiology

authors： Kikumoto M,Nogami S,Kanao T,Takada J,Kamimura K

更新日期：2013-01-01 00:00:00

abstract：:A selective and differential growth medium was developed for detection of Clostridium botulinum types A, B, and F. The medium consisted of peptone-glucose-yeast extract agar supplemented with cycloserine, 250 micrograms/ml; sulfamethoxazole, 76 micrograms/ml; and trimethoprim, 4 micrograms/ml as selective inhibitors a...

journal_title：Applied and environmental microbiology

authors： Silas JC,Carpenter JA,Hamdy MK,Harrison MA

更新日期：1985-10-01 00:00:00

abstract：:Two types of mesophilic, methanogenic bacteria were isolated in pure culture from anaerobic freshwater and marine mud with 2-propanol as the hydrogen donor. The freshwater strain (SK) was a Methanospirillum species, the marine, salt-requiring strain (CV), which had irregular coccoid cells, resembled Methanogenium sp. ...

journal_title：Applied and environmental microbiology

authors： Widdel F

更新日期：1986-05-01 00:00:00

abstract：:Bacterial plasmids are fragments of extrachromosomal double-stranded DNA that can contain a variety of genes that are beneficial to the host organism, like those responsible for antimicrobial resistance. The objective of this study was to characterize a collection of 437 Salmonella enterica isolates from different ani...

journal_title：Applied and environmental microbiology

authors： Lindsey RL,Fedorka-Cray PJ,Frye JG,Meinersmann RJ

更新日期：2009-04-01 00:00:00

abstract：:Throughout four field trials, compost heaps covered with finished compost maintained temperatures under the physical covering that were ca. 7 to 15.5°C higher, resulting in rapid Escherichia coli O157:H7 reduction, than those of the heaps covered with fresh straw or left uncovered. Our results validated recommendation...

journal_title：Applied and environmental microbiology

authors： Shepherd MW Jr,Kim J,Jiang X,Doyle MP,Erickson MC

更新日期：2011-07-01 00:00:00

abstract：:A unique lipolytic bacterium was isolated in a selective growth system consisting of 99% triglycerides and a 1% water phase. The bacterium, termed Pseudomonas aeruginosa YS-7, was able to grow in an environment of low water content and could also survive amphipathic, osmotic, and matrical water stress in a triglycerid...

journal_title：Applied and environmental microbiology

authors： Shabtai Y

更新日期：1991-06-01 00:00:00

abstract：:Ceftiofur, a third-generation cephalosporin antimicrobial, was used in Canadian hatcheries for many years to prevent early mortality in chicks, leading to a high prevalence of cephalosporin resistance in Escherichia coli in chickens. Preventive use of ceftiofur in hatcheries ceased in 2014. We examined the effect of c...

journal_title：Applied and environmental microbiology

authors： Verrette L,Fairbrother JM,Boulianne M

更新日期：2019-06-17 00:00:00

abstract：:Lactobacillus species normally found in the intestinal tract of humans varied in the ability to deconjugate bile acids, whereas laboratory strains of Lactobacillus acidophilus deconjugated both glycocholate and taurocholate. All isolates of L. acidophilus from human feces deconjugated taurocholate, whereas only one of...

journal_title：Applied and environmental microbiology

authors： Gilliland SE,Speck ML

更新日期：1977-01-01 00:00:00

abstract：:Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...

journal_title：Applied and environmental microbiology

authors： Ayele WY,Svastova P,Roubal P,Bartos M,Pavlik I

更新日期：2005-03-01 00:00:00

abstract：:Submerged cultures of Penicillium urticae (NRRL 2159A) produced the antibiotics patulin and griseofulvin when grown in a glucose-nitrate medium. A high concentration of calcium (i.e., 68 mM) inhibited the production of both antibiotics while stimulating conidiogenesis. Conidial mutants that were defective in an early ...

journal_title：Applied and environmental microbiology

authors： Sekiguchi J,Gaucher GM

更新日期：1977-01-01 00:00:00

abstract：:β-Aminopeptidases have the unique capability to hydrolyze N-terminal β-amino acids, with varied preferences for the nature of β-amino acid side chains. This unique capability makes them useful as biocatalysts for synthesis of β-peptides and to kinetically resolve β-peptides and amides for the production of enantiopure...

journal_title：Applied and environmental microbiology

authors： John-White M,Gardiner J,Johanesen P,Lyras D,Dumsday G

更新日期：2019-07-18 00:00:00

abstract：:The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow th...

journal_title：Applied and environmental microbiology

authors： Sandu C,Chiribau CB,Sachelaru P,Brandsch R

更新日期：2005-12-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored t...

journal_title：Applied and environmental microbiology

authors： Guimarães VD,Innocentin S,Lefèvre F,Azevedo V,Wal JM,Langella P,Chatel JM

更新日期：2006-11-01 00:00:00

abstract：:Lantibiotics are antimicrobial peptides belonging to the family of ribosomally synthesized and posttranslationally modified peptides (RiPPs) and feature thioether linkages in their structures. In this study, we identified the biosynthetic gene cluster of a cinnamycin analog, named mathermycin, from Marinactinospora th...

journal_title：Applied and environmental microbiology

authors： Chen E,Chen Q,Chen S,Xu B,Ju J,Wang H

更新日期：2017-07-17 00:00:00

abstract：:Predicting the presence of enteric viruses in surface waters is a complex modeling problem. Multiple water quality parameters that indicate the presence of human fecal material, the load of fecal material, and the amount of time fecal material has been in the environment are needed. This paper presents the results of ...

journal_title：Applied and environmental microbiology

authors： Black LE,Brion GM,Freitas SJ

更新日期：2007-06-01 00:00:00

abstract：:Tn5 insertion mutants of a soil isolate, Pseudomonas fluorescens Pf0-1, were selected for decreased ability to adhere to quartz sand in a column assay. Three adhesion-deficient mutants that differed in the location of the Tn5 insertion in the chromosome were isolated and compared with the wild-type strain. One mutant,...

journal_title：Applied and environmental microbiology

authors： Deflaun MF,Marshall BM,Kulle EP,Levy SB

更新日期：1994-07-01 00:00:00

abstract：:Xanthobacter flavus 14p1 was isolated from sludge of the river Mulde by selective enrichment with 1,4-dichlorobenzene as the sole source of carbon and energy. The bacterium did not use other aromatic or chloroaromatic compounds as growth substrates. During growth on 1,4-dichlorobenzene, stoichiometric amounts of chlor...

journal_title：Applied and environmental microbiology

authors： Spiess E,Sommer C,Görisch H

更新日期：1995-11-01 00:00:00

abstract：:Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of CD. The aim of this study was to characterize the composition and diversity of the cult...

journal_title：Applied and environmental microbiology

authors： Sánchez E,Donat E,Ribes-Koninckx C,Fernández-Murga ML,Sanz Y

更新日期：2013-09-01 00:00:00

abstract：:The subunit composition of the extracellular complex from Clostridium thermocellum was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-six bands, representing proteins with apparent molecular sizes ranging from 37,500 to 185,000 Da, could be detected by silver staining. Cultiva...

journal_title：Applied and environmental microbiology

authors： Kohring S,Wiegel J,Mayer F

更新日期：1990-12-01 00:00:00

abstract：:A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy s...

journal_title：Applied and environmental microbiology

authors： Marks TS,Smith AR,Quirk AV

更新日期：1984-11-01 00:00:00

abstract：:Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while...

journal_title：Applied and environmental microbiology

authors： Deng MQ,Cliver DO

更新日期：1998-05-01 00:00:00

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that ...

journal_title：Applied and environmental microbiology

authors： Huber H,Stetter KO

更新日期：1990-02-01 00:00:00

abstract：:Phage therapy is being reexamined as a strategy for bacterial control in medical and other environments. As microorganisms often live in mixed populations, we examined the effect of Escherichia coli bacteriophage λW60 and Pseudomonas aeruginosa bacteriophage PB-1 infection on the viability of monoculture and mixed-spe...

journal_title：Applied and environmental microbiology

authors： Kay MK,Erwin TC,McLean RJ,Aron GM

更新日期：2011-02-01 00:00:00

abstract：:Hartmannella vermiformis, a common amoebal inhabitant of potable-water systems, supports intracellular multiplication of Legionella pneumophila and is probably important in the transportation and amplification of legionellae within these systems. To provide a practical guide for decontamination of potable-water system...

journal_title：Applied and environmental microbiology

authors： Kuchta JM,Navratil JS,Shepherd ME,Wadowsky RM,Dowling JN,States SJ,Yee RB

更新日期：1993-12-01 00:00:00

abstract：:Streptococcus suis is an important pathogen of swine which occasionally infects humans as well. There are 35 serotypes known for this organism, and it would be desirable to develop rapid methods methods to identify and differentiate the strains of this species. To that effect, partial chaperonin 60 gene sequences were...

journal_title：Applied and environmental microbiology

authors： Brousseau R,Hill JE,Préfontaine G,Goh SH,Harel J,Hemmingsen SM

更新日期：2001-10-01 00:00:00