Production of respirable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp.

Abstract:

:Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two cooling tower biocides for 24 h. Clusters of bacteria in vesicles were not dispersed by freeze-thawing and sonication. Such vesicles may be agents for the transmission of legionellosis associated with cooling towers, and the risk may be underestimated by plate count methods.

journal_name

Appl Environ Microbiol

authors

Berk SG,Ting RS,Turner GW,Ashburn RJ

doi

10.1128/AEM.64.1.279-286.1998

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

279-86

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

64

pub_type

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