Selective and differential medium for detecting Clostridium botulinum.

Abstract:

:A selective and differential growth medium was developed for detection of Clostridium botulinum types A, B, and F. The medium consisted of peptone-glucose-yeast extract agar supplemented with cycloserine, 250 micrograms/ml; sulfamethoxazole, 76 micrograms/ml; and trimethoprim, 4 micrograms/ml as selective inhibitors and various types and levels of botulinal antibodies for type differentiation in the immunodiffusion reaction. Growth of proteolytic types of C. botulinum were not affected by the incorporation of the selective agents, but some nonproteolytic types were suppressed. Cross-reactions between types A and B were visually distinguishable, whereas cross-reactions between type F and Clostridium sporogenes did not occur at the optimum antibody titer. Optimum antibody titer varied with toxin type. The proposed selective differential medium should be valuable in isolating and typing of proteolytic C. botulinum types A, B, and F from samples containing mixed microbial populations.

journal_name

Appl Environ Microbiol

authors

Silas JC,Carpenter JA,Hamdy MK,Harrison MA

doi

10.1128/AEM.50.4.1110-1111.1985

subject

Has Abstract

pub_date

1985-10-01 00:00:00

pages

1110-1

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

50

pub_type

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