Development of a reverse genetic system for infectious salmon anemia virus: rescue of recombinant fluorescent virus by using salmon internal transcribed spacer region 1 as a novel promoter.

abstract：:Environmental contamination with compounds containing oxyanions of chlorine, such as perchlorate or chlorate [(per)chlorate] or chlorine dioxide, has been a constantly growing problem over the last 100 years. Although the fact that microbes reduce these compounds has been recognized for more than 50 years, only six or...

journal_title：Applied and environmental microbiology

authors： Coates JD,Michaelidou U,Bruce RA,O'Connor SM,Crespi JN,Achenbach LA

更新日期：1999-12-01 00:00:00

abstract：:We previously showed a pivotal role of the polysaccharide (PS) moiety in the cell wall of the Lactobacillus casei strain Shirota (YIT 9029) as a possible immune modulator (E. Yasuda M. Serata, and T. Sako, Appl. Environ. Microbiol. 74:4746-4755, 2008). To distinguish PS structures on the bacterial cell surface of indi...

journal_title：Applied and environmental microbiology

authors： Yasuda E,Tateno H,Hirabayashi J,Iino T,Sako T

更新日期：2011-07-01 00:00:00

abstract：:Mass spectrometric measurement of carbon dioxide production was used to study malolactic fermentation (MLF) in Lactobacillus collinoides isolated from cider. The kinetics and stereospecificity of the malolactic enzyme (MLE) were studied, and the stoichiometry of the reaction sequence was investigated. The optimum pH f...

journal_title：Applied and environmental microbiology

authors： Arthurs CE,Lloyd D

更新日期：1999-08-01 00:00:00

abstract：:Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial commun...

journal_title：Applied and environmental microbiology

authors： Poppert S,Essig A,Marre R,Wagner M,Horn M

更新日期：2002-08-01 00:00:00

abstract：:Bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (Aulopiformes: Chlorophthalmidae) are considered to belong to the species Photobacterium phosphoreum. The identification of these bacteria as P. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate betwe...

journal_title：Applied and environmental microbiology

authors： Dunlap PV,Ast JC

更新日期：2005-02-01 00:00:00

abstract：:In this study, we used chemostat cultures to analyze the quantitative effects of the specific growth rate and respiration on the metabolism in Lactococcus lactis CHCC2862 and on the downstream robustness of cells after freezing or freeze-drying. Under anaerobic conditions, metabolism remained homofermentative, althoug...

journal_title：Applied and environmental microbiology

authors： Johanson A,Goel A,Olsson L,Franzén CJ

更新日期：2020-03-02 00:00:00

abstract：:A yellow-pigmented marine bacterium, designated strain SD-21, was isolated from surface sediments of San Diego Bay, San Diego, Calif., based on its ability to oxidize soluble Mn(II) to insoluble Mn(III, IV) oxides. 16S rRNA analysis revealed that this organism was most closely related to members of the genus Erythroba...

journal_title：Applied and environmental microbiology

authors： Francis CA,Co EM,Tebo BM

更新日期：2001-09-01 00:00:00

abstract：:A strain of the aerobic anoxygenic photosynthetic bacteria was isolated from a deep-ocean hydrothermal vent plume environment. The in vivo absorption spectra of cells indicate the presence of bacteriochlorophyll a incorporated into light-harvesting complex I and a reaction center. The general morphological and physiol...

journal_title：Applied and environmental microbiology

authors： Yurkov V,Beatty JT

更新日期：1998-01-01 00:00:00

abstract：:Five cases of primary amoebic meningoencephalitis associated with swimming in freshwater lakes have been recorded in Florida over the past 14 years. The present study demonstrated that pathogenic Naegleria, the causative agent, is relatively widespread. Twelve of 26 lakes sampled only once yielded the amoeba. Populati...

journal_title：Applied and environmental microbiology

authors： Wellings FM,Amuso PT,Chang SL,Lewis AL

更新日期：1977-12-01 00:00:00

abstract：:Acid-adapted strains of Escherichia coli K-12 W3110 were obtained by serial culture in medium buffered at pH 4.6 (M. M. Harden, A. He, K. Creamer, M. W. Clark, I. Hamdallah, K. A. Martinez, R. L. Kresslein, S. P. Bush, and J. L. Slonczewski, Appl Environ Microbiol 81:1932-1941, 2015, https://doi.org/10.1128/AEM.03494-...

journal_title：Applied and environmental microbiology

authors： He A,Penix SR,Basting PJ,Griffith JM,Creamer KE,Camperchioli D,Clark MW,Gonzales AS,Chávez Erazo JS,George NS,Bhagwat AA,Slonczewski JL

更新日期：2017-05-31 00:00:00

abstract：:Lignocellulose digestion by wood-feeding termites depends on the mutualistic interaction of unusual, flagellate protists located in their hindgut. Most of the flagellates harbor numerous prokaryotic endosymbionts of so-far-unknown identity and function. Using a full-cycle molecular approach, we show here that the endo...

journal_title：Applied and environmental microbiology

authors： Stingl U,Radek R,Yang H,Brune A

更新日期：2005-03-01 00:00:00

abstract：:Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of...

journal_title：Applied and environmental microbiology

authors： Klein PG,Juneja VK

更新日期：1997-11-01 00:00:00

abstract：:Trichloroethylene (TCE)-transforming aquifer methanotrophs were evaluated for the influence of TCE oxidation toxicity and the effect of reductant availability on TCE transformation rates during methane starvation. TCE oxidation at relatively low (6 mg liter-1) TCE concentrations significantly reduced subsequent methan...

journal_title：Applied and environmental microbiology

authors： Henry SM,Grbić-Galić D

更新日期：1991-01-01 00:00:00

abstract：:The fate and impact of Pseudomonas aureofaciens TX-1 following application as a biocontrol agent for fungi in turfgrass were studied. The organism was applied with a modified irrigation system by using a preparation containing 1 x 10(6) P. aureofaciens TX-1 CFU ml(-1) about 100 times between May and August. We examine...

journal_title：Applied and environmental microbiology

authors： Sigler WV,Nakatsu CH,Reicher ZJ,Turco RF

更新日期：2001-08-01 00:00:00

abstract：:The physiology of filamentous fungi at growth rates approaching zero has been subject to limited study and exploitation. With the aim of uncoupling product formation from growth, we have revisited and improved the retentostat cultivation method for Aspergillus niger. A new retention device was designed allowing reliab...

journal_title：Applied and environmental microbiology

authors： Jørgensen TR,Nitsche BM,Lamers GE,Arentshorst M,van den Hondel CA,Ram AF

更新日期：2010-08-01 00:00:00

abstract：:Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

journal_title：Applied and environmental microbiology

authors： Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

更新日期：2017-05-17 00:00:00

abstract：:To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a hig...

journal_title：Applied and environmental microbiology

authors： Yan J,Han XZ,Ji ZJ,Li Y,Wang ET,Xie ZH,Chen WF

更新日期：2014-09-01 00:00:00

abstract：:Citrate metabolism by Enterococcus faecium FAIR-E 198, an isolate from Greek Feta cheese, was studied in modified MRS (mMRS) medium under different pH conditions and glucose and citrate concentrations. In the absence of glucose, this strain was able to metabolize citrate in a pH range from constant pH 5.0 to 7.0. At a...

journal_title：Applied and environmental microbiology

authors： Vaningelgem F,Ghijsels V,Tsakalidou E,De Vuyst L

更新日期：2006-01-01 00:00:00

abstract：:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...

journal_title：Applied and environmental microbiology

authors： De Oliveira VC,Maeda I,Delessert S,Poirier Y

更新日期：2004-09-01 00:00:00

abstract：:Current methods for detection of Cryptosporidium parvum oocysts in water are time-consuming and difficult. We have developed a reverse transcription (RT)-PCR which can detect the presence of a single viable oocyst spiked into concentrated environmental water samples. The test is based on the detection of mRNA from a C...

journal_title：Applied and environmental microbiology

authors： Stinear T,Matusan A,Hines K,Sandery M

更新日期：1996-09-01 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:Microbial communities from a subseafloor sediment core from the southwestern Sea of Okhotsk were evaluated by performing both cultivation-dependent and cultivation-independent (molecular) analyses. The core, which extended 58.1 m below the seafloor, was composed of pelagic clays with several volcanic ash layers contai...

journal_title：Applied and environmental microbiology

authors： Inagaki F,Suzuki M,Takai K,Oida H,Sakamoto T,Aoki K,Nealson KH,Horikoshi K

更新日期：2003-12-01 00:00:00

abstract：:Cultures of Anabaena variabilis were exposed to different light intensities, and the time course of photoadaptation was measured by photosynthetic rate and changes in pigmentation. A shift down in intensity of 284 muEin . m . sec caused a temporary decrease in the photosynthetic response followed by gradual adaptation...

journal_title：Applied and environmental microbiology

authors： Collins CD,Boylen CW

更新日期：1982-07-01 00:00:00

abstract：:Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other ty...

journal_title：Applied and environmental microbiology

authors： Rezzonico F,Défago G,Moënne-Loccoz Y

更新日期：2004-09-01 00:00:00

abstract：:Fifty-one isolates of Rhizobium leguminosarum biovar phaseoli from various geographic and ecological sources, largely in Mexico, were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 46 distinctive multilocus genotypes (electrophoretic types [ETs]) were distinguished on the basis of allele ...

journal_title：Applied and environmental microbiology

authors： Pinero D,Martinez E,Selander RK

更新日期：1988-11-01 00:00:00

abstract：:The siderophore synthetic system in Shewanella species is able to synthesize dozens of macrocyclic siderophores in vitro with synthetic precursors. In vivo, however, although three siderophores are produced naturally in Shewanella algae B516, which carries a lysine decarboxylase (AvbA) specific for siderophore synthes...

journal_title：Applied and environmental microbiology

authors： Wang S,Liang H,Liu L,Jiang X,Wu S,Gao H

更新日期：2020-03-18 00:00:00

abstract：:Mean pH values in pooled samples of foregut, midgut, and hindgut from adult Melanoplus sanguinipes, which had been raised in the laboratory on barley shoots and wheat bran, were 5.15, 6.39, and 5.98, respectively. Homogenates of midgut/hindgut sections and frass (feces) yielded colony counts of bacteria by the spread ...

journal_title：Applied and environmental microbiology

authors： Mead LJ,Khachatourians GG,Jones GA

更新日期：1988-05-01 00:00:00

abstract：:This is the first study to characterize the environmental conditions which contribute to the presence and proliferation of environmental mycobacteria in a major freshwater river. Over 20 different species of environmental mycobacteria were isolated, including the pathogenic M. avium and M. kansasii. Species of the rap...

journal_title：Applied and environmental microbiology

authors： Bland CS,Ireland JM,Lozano E,Alvarez ME,Primm TP

更新日期：2005-10-01 00:00:00

abstract：:A gram-negative bacterium which hydrolyzed aryl N-methylcarbamate insecticides was isolated from an agricultural soil which quickly degraded these pesticides. This organism, designated strain ER2, grew on carbofuran as a sole source of carbon and nitrogen with a doubling time of 3 h in a mineral salts medium. The arom...

journal_title：Applied and environmental microbiology

authors： Topp E,Hanson RS,Ringelberg DB,White DC,Wheatcroft R

更新日期：1993-10-01 00:00:00

abstract：:The release rate of dissolved organic carbon (DOC) by unialgal cultures and natural phytoplankton assemblages was constant over a wide range of dissolved inorganic carbon concentrations. DOC release was not proportional to the particulate organic carbon production rate. We postulate that intracellular DOC, fated for r...

journal_title：Applied and environmental microbiology

authors： Smith DF,Wiebe WJ

更新日期：1976-07-01 00:00:00