Abstract:
:Trichloroethylene (TCE)-transforming aquifer methanotrophs were evaluated for the influence of TCE oxidation toxicity and the effect of reductant availability on TCE transformation rates during methane starvation. TCE oxidation at relatively low (6 mg liter-1) TCE concentrations significantly reduced subsequent methane utilization in mixed and pure cultures tested and reduced the number of viable cells in the pure culture Methylomonas sp. strain MM2 by an order of magnitude. Perchloroethylene, tested at the same concentration, had no effect on the cultures. Neither the TCE itself nor the aqueous intermediates were responsible for the toxic effect, and it is suggested that TCE oxidation toxicity may have resulted from reactive intermediates that attacked cellular macromolecules. During starvation, all methanotrophs tested exhibited a decline in TCE transformation rates, and this decline followed exponential decay. Formate, provided as an exogenous electron donor, increased TCE transformation rates in Methylomonas sp. strain MM2, but not in mixed culture MM1 or unidentified isolate, CSC-1. Mixed culture MM2 did not transform TCE after 15 h of starvation, but mixed cultures MM1 and MM3 did. The methanotrophs in mixed cultures MM1 and MM3, and the unidentified isolate CSC-1 that was isolated from mixed culture MM1 contained lipid inclusions, whereas the methanotrophs of mixed culture MM2 and Methylomonas sp. strain MM2 did not. It is proposed that lipid storage granules serve as an endogenous source of electrons for TCE oxidation during methane starvation.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Henry SM,Grbić-Galić Ddoi
10.1128/AEM.57.1.236-244.1991subject
Has Abstractpub_date
1991-01-01 00:00:00pages
236-44issue
1eissn
0099-2240issn
1098-5336journal_volume
57pub_type
杂志文章abstract::The ability of certain oral biofilm bacteria to moderate pH through arginine metabolism by the arginine deiminase system (ADS) is a deterrent to the development of dental caries. Here, we characterize a novel Streptococcus strain, designated strain A12, isolated from supragingival dental plaque of a caries-free indivi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03887-15
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doi:10.1128/AEM.02848-07
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pub_type: 杂志文章
doi:10.1128/AEM.53.8.1898-1901.1987
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pub_type: 杂志文章
doi:10.1128/AEM.01675-13
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doi:10.1128/AEM.02542-15
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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更新日期:1985-09-01 00:00:00
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更新日期:1984-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.10.2458-2463.1987
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.2.568-572.1991
更新日期:1991-02-01 00:00:00
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pub_type: 杂志文章
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doi:10.1128/AEM.37.3.544-549.1979
更新日期:1979-03-01 00:00:00
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doi:10.1128/AEM.45.2.628-633.1983
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.4.1201-1206.1992
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.31.6.949-958.1976
更新日期:1976-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.5.1291-1294.1989
更新日期:1989-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2018-02-14 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.1.278-283.1995
更新日期:1995-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.11.3939-3947.1996
更新日期:1996-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00829-19
更新日期:2019-09-17 00:00:00