Abstract:
:Natural denitrification rates and activities of denitrifying enzymes were measured in an agricultural soil which had a 20-year past history of low pH (pH ca. 4) due to fertilization with acid-generating ammonium salts. The soil adjacent to this site had been limed and had a pH of ca. 6.0. Natural denitrification rates of these areas were of similar magnitude: 158 ng of N g of soil day for the acid soil and 390 ng of N g of soil day at the neutral site. Estimates of in situ denitrifying enzyme activity were higher in the neutral soil, but substantial enzyme activity was also detected in the acid soil. Rates of nitrous oxide reduction were very low, even when NO(3) and NO(2) were undetectable, and were ca. 400 times lower than the rates of N(2)O production from NO(3). Denitrification rates measured in slurries of the acid and neutral soil showed distinctly different pH optima (pH 3.9 and pH 6.3) which were near the pH values of the two soils. This suggests that an acid-tolerant denitrifying population had been selected during the 20-year period of low pH.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Parkin TB,Sexstone AJ,Tiedje JMdoi
10.1128/AEM.49.5.1053-1056.1985keywords:
subject
Has Abstractpub_date
1985-05-01 00:00:00pages
1053-6issue
5eissn
0099-2240issn
1098-5336journal_volume
49pub_type
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