Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds.

abstract：:Three expedition huts in the Ross Sea region of Antarctica, built between 1901 and 1911 by Robert F. Scott and Ernest Shackleton, sheltered and stored the supplies for up to 48 men for 3 years during their explorations and scientific investigation in the South Pole region. The huts, built with wood taken to Antarctica...

journal_title：Applied and environmental microbiology

authors： Blanchette RA,Held BW,Jurgens JA,McNew DL,Harrington TC,Duncan SM,Farrell RL

更新日期：2004-03-01 00:00:00

abstract：:Of 199 fungal cultures isolated from some of the common cereals collected from different parts of Uttar Pradesh and Madhya Pradesh, 70 produced toxic metabolites. Of the 70 fungi isolated, 59 produced toxins which caused visible lesions in livers, kidneys, and spleens but did not cause mortality. Toxicity, graded in t...

journal_title：Applied and environmental microbiology

authors： Gupta J,Pathak B,Sethi N,Vora VC

更新日期：1981-03-01 00:00:00

abstract：:The viability of starved or dying Saccharomyces cerevisiae Y5 cells was extended for up to 1,500 h by adding osmotic supports (0.6 M sorbitol or mannitol) to the starvation media. Replacement of these polyols with 0.3 M KCl, 0.5 M MgSO4, or 0.2 M MgCl2 postponed the death of cells incubated in N-free medium, but accel...

journal_title：Applied and environmental microbiology

authors： Peled ON

更新日期：1985-09-01 00:00:00

abstract：:Deletion of chromosomally inserted gene sequences from Clavibacter xyli subsp. cynodontis, a xylem-inhabiting endophyte, was studied in vitro and in planta. We found that nonreplicating plasmid pCG610, which conferred resistance to kanamycin and tetracycline and contained segments of C. xyli subsp. cynodontis genomic ...

journal_title：Applied and environmental microbiology

authors： Turner JT,Lampel JS,Stearman RS,Sundin GW,Gunyuzlu P,Anderson JJ

更新日期：1991-12-01 00:00:00

abstract：:Xanthomonas citri subsp. citri is the causal agent of citrus canker, which has a significant impact on citrus production. In this study, we characterized the galU gene of X. citri subsp. citri. Two galU mutants (F6 and D12) were identified in an X. citri subsp. citri EZ-Tn5 Tnp transposon library. ...

journal_title：Applied and environmental microbiology

authors： Guo Y,Sagaram US,Kim JS,Wang N

更新日期：2010-04-01 00:00:00

abstract：:This study measured the environmental variability which exists in a commercial aerobic composting process. The specific process studied is carried out in six decomposition cells which present six different phases of the process. Temperature, pH, and moisture content were determined in several randomly chosen sample si...

journal_title：Applied and environmental microbiology

authors： Atchley SH,Clark JB

更新日期：1979-12-01 00:00:00

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:Cyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogen-limiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand ...

journal_title：Applied and environmental microbiology

authors： Jackson SA,Eaton-Rye JJ,Bryant DA,Posewitz MC,Davies FK

更新日期：2015-09-01 00:00:00

abstract：:Campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. This procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of C. jejuni in milk from bulk tanks. ...

journal_title：Applied and environmental microbiology

authors： Lovett J,Francis DW,Hunt JM

更新日期：1983-08-01 00:00:00

abstract：:A new thermostable dipeptidase gene was cloned from the thermophile Brevibacillus borstelensis BCS-1 by genetic complementation of the D-Glu auxotroph Escherichia coli WM335 on a plate containing D-Ala-D-Glu. Nucleotide sequence analysis revealed that the gene included an open reading frame coding for a 307-amino-acid...

journal_title：Applied and environmental microbiology

authors： Baek DH,Song JJ,Kwon SJ,Park C,Jung CM,Sung MH

更新日期：2004-03-01 00:00:00

abstract：:Although at least 13 antigenically distinct serotypes of Rhizobium leguminosarum bv. trifolii exist in an Abiqua silty clay loam soil, one serotype, AS6, occupies >/=50% of the root nodules formed on field-grown subclover and between 33 and 78% of the nodules formed on five annual clover species grown in the same soil...

journal_title：Applied and environmental microbiology

authors： Leung K,Yap K,Dashti N,Bottomley PJ

更新日期：1994-02-01 00:00:00

abstract：:The colonization of apple blossoms and leaves by Pseudomonas fluorescens EPS62e was monitored in greenhouse and field trials using cultivable cell counting and real-time PCR. The real-time PCR provided a specific quantitative method for the detection of strain EPS62e. The detection level was around 10(2) cells g (fres...

journal_title：Applied and environmental microbiology

authors： Pujol M,Badosa E,Manceau C,Montesinos E

更新日期：2006-04-01 00:00:00

abstract：:In the luminal contents of metronidazole-treated rats, there was a dominant Bifidobacterium species. A strain has been isolated, its 16S rRNA gene has been sequenced, and the strain has been named Bifidobacterium pseudolongum strain Patronus. In this study, using an experimental model of healthy rats, the effects of m...

journal_title：Applied and environmental microbiology

authors： Vasquez N,Suau A,Magne F,Pochart P,Pélissier MA

更新日期：2009-01-01 00:00:00

abstract：:DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific prob...

journal_title：Applied and environmental microbiology

authors： Shin BS,Park SH,Choi SK,Koo BT,Lee ST,Kim JI

更新日期：1995-06-01 00:00:00

abstract：:Dibenzothiophene is degraded to 3-hydroxy-2-formyl benzothiophene by various bacteria, including a strain of Pseudomonas putida that also forms dibenzothiophene sulfone via an alternate pathway. By using these end products as substrates, mixed enrichment cultures that could degrade 3-hydroxy-2-formyl benzothiophene an...

journal_title：Applied and environmental microbiology

authors： Mormile MR,Atlas RM

更新日期：1988-12-01 00:00:00

abstract：:To observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (Danio rerio), a series of plasmids was constructed for the red fluorescent protein (RFP) labeling of a variety of fish and human pathogens. The aim of this study was to create a collection o...

journal_title：Applied and environmental microbiology

authors： Singer JT,Phennicie RT,Sullivan MJ,Porter LA,Shaffer VJ,Kim CH

更新日期：2010-06-01 00:00:00

abstract：:A gene coding for an aminopeptidase (PepC) from Lactococcus lactis subsp. cremoris AM2 was cloned by complementation of an Escherichia coli mutant lacking aminopeptidase activity. The nucleotide sequence was determined. A portion of the predicted amino acid sequence of PepC (436 amino acids) showed strong homology to ...

journal_title：Applied and environmental microbiology

authors： Chapot-Chartier MP,Nardi M,Chopin MC,Chopin A,Gripon JC

更新日期：1993-01-01 00:00:00

abstract：:Research on the microbial communities that colonize stone monuments may provide a new understanding of stone biodeterioration and microbe-induced carbonate precipitation. This work investigated the seasonal variation of microbial communities in 2016 and 2017, as well as its effects on stone monuments. We determined th...

journal_title：Applied and environmental microbiology

authors： Li Q,Zhang B,Yang X,Ge Q

更新日期：2018-03-19 00:00:00

abstract：:This study describes the genotypic characteristics of a collection of 100 multidrug-resistant (MDR) Escherichia coli strains recovered from cattle and the farm environment in Ireland in 2007. The most prevalent antimicrobial resistance identified was to streptomycin (100%), followed by tetracycline (99%), sulfonamides...

journal_title：Applied and environmental microbiology

authors： Karczmarczyk M,Walsh C,Slowey R,Leonard N,Fanning S

更新日期：2011-10-01 00:00:00

abstract：:In this work we investigated the antibacterial properties of differently shaped silver nanoparticles against the gram-negative bacterium Escherichia coli, both in liquid systems and on agar plates. Energy-filtering transmission electron microscopy images revealed considerable changes in the cell membranes upon treatme...

journal_title：Applied and environmental microbiology

authors： Pal S,Tak YK,Song JM

更新日期：2007-03-01 00:00:00

abstract：:Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

journal_title：Applied and environmental microbiology

authors： Tompkin RB,Christiansen LN,Shaparis AB

更新日期：1978-01-01 00:00:00

abstract：:Vibrio vulnificus is a human and animal pathogen that carries the highest death rate of any food-borne disease agent. It colonizes shellfish and forms biofilms on the surfaces of plankton, algae, fish, and eels. Greater understanding of biofilm formation by the organism could provide insight into approaches to decreas...

journal_title：Applied and environmental microbiology

authors： Nakhamchik A,Wilde C,Rowe-Magnus DA

更新日期：2008-07-01 00:00:00

abstract：:Based on results from eight experiments, no overall difference was found between roll tube and three- and five-tube most-probable-number (MPN) methods for estimating total numbers of ruminal bacteria. However, standard errors for the replicate means within an experiment were higher with the MPN procedures. Visual grow...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA,Grifo AP Jr

更新日期：1989-11-01 00:00:00

abstract：:Human noroviruses are excreted in feces from infected individuals and included in wastewater. It is critical to remove/inactivate them in wastewater treatment processes, particularly in the disinfection step, before release to aquatic environments. However, the high mutation rates of human noroviruses raise concerns a...

journal_title：Applied and environmental microbiology

authors： Rachmadi AT,Kitajima M,Watanabe K,Yaegashi S,Serrana J,Nakamura A,Nakagomi T,Nakagomi O,Katayama K,Okabe S,Sano D

更新日期：2018-06-18 00:00:00

abstract：:Healthy calves (n = 96, 1 to 9 weeks old) from a dairy herd in central Pennsylvania were examined each month over a five-month period for fecal shedding of ceftiofur-resistant gram-negative bacteria. Ceftiofur-resistant Escherichia coli isolates (n = 122) were characterized by antimicrobial resistance (disk diffusion ...

journal_title：Applied and environmental microbiology

authors： Donaldson SC,Straley BA,Hegde NV,Sawant AA,DebRoy C,Jayarao BM

更新日期：2006-06-01 00:00:00

abstract：:A longstanding awareness in generating resistance to common antimicrobial therapies by Gram-negative bacteria has made them a major threat to global health. The application of antimicrobial peptides as a therapeutic agent would be a great opportunity to combat bacterial diseases. Here, we introduce a new antimicrobial...

journal_title：Applied and environmental microbiology

authors： Modiri S,Kasra Kermanshahi R,Soudi MR,Arab SS,Khammari A,Cousineau B,Vali H,Zahiri HS,Noghabi KA

更新日期：2020-05-05 00:00:00

abstract：:DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...

journal_title：Applied and environmental microbiology

authors： Huys G,D'Haene K,Van Eldere J,von Holy A,Swings J

更新日期：2005-01-01 00:00:00

abstract：:The potential for aerobic methyl tert-butyl ether (MTBE) degradation was investigated with microcosms containing aquifer sediment and groundwater from four MTBE-contaminated sites characterized by oxygen-limited in situ conditions. MTBE depletion was observed for sediments from two sites (e.g., 4.5 mg/liter degraded i...

journal_title：Applied and environmental microbiology

authors： Kane SR,Beller HR,Legler TC,Koester CJ,Pinkart HC,Halden RU,Happel AM

更新日期：2001-12-01 00:00:00

abstract：:Detection of psychrotrophic strains (those able to grow at or below 7 degreesC) of the Bacillus cereus group (Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides) in food products is at present extremely slow with conventional microbiology. This is due to an inability to discriminate these cold-adapted stra...

journal_title：Applied and environmental microbiology

authors： Francis KP,Mayr R,von Stetten F,Stewart GS,Scherer S

更新日期：1998-09-01 00:00:00

abstract：:The consumption of acetate in soils taken from a nutrient gradient in the northern Florida Everglades was studied by stable isotope probing. Bacterial and archaeal 16S rRNA gene clone libraries from eutrophic and oligotrophic soil microcosms strongly suggest that a significant amount of acetate is consumed by syntroph...

journal_title：Applied and environmental microbiology

authors： Chauhan A,Ogram A

更新日期：2006-10-01 00:00:00