Molecular characterization of multidrug-resistant Escherichia coli isolates from Irish cattle farms.

abstract：:Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

journal_title：Applied and environmental microbiology

authors： Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

更新日期：2012-07-01 00:00:00

abstract：:During growth on xylan and xylose Thermoanaerobacterium saccharolyticum B6A-RI produced endoxylanase, beta-xylosidase, arabinofuranosidase, and acetyl esterase, and the first three activities appeared to be produced coordinately. During nonlimiting growth on xylan, these enzyme activities were predominantly cell assoc...

journal_title：Applied and environmental microbiology

authors： Lee YE,Lowe SE,Zeikus JG

更新日期：1993-03-01 00:00:00

abstract：:Since 2006, Geomyces destructans, the causative agent of white nose syndrome (WNS), has killed over 5.7 million bats in North America. The current hypothesis suggests that this novel fungus is an invasive species from Europe, but little is known about the diversity within the genus Geomyces and its distribution on bat...

journal_title：Applied and environmental microbiology

authors： Johnson LJ,Miller AN,McCleery RA,McClanahan R,Kath JA,Lueschow S,Porras-Alfaro A

更新日期：2013-09-01 00:00:00

abstract：:"Caldocellum saccharolyticum" is an obligatory anaerobic thermophilic bacterium. A gene from this organism, designated celB, has been cloned in Escherichia coli as part of a bacteriophage lambda gene library. This gene produces a thermostable cellulase that shows both endoglucanase and exoglucanase activities on test ...

journal_title：Applied and environmental microbiology

authors： Saul DJ,Williams LC,Grayling RA,Chamley LW,Love DR,Bergquist PL

更新日期：1990-10-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:Cattle are a known reservoir of Shiga toxin-producing Escherichia coli. The prevalence and stability of Shiga toxin and/or Shiga toxin genes among native wild ungulates in Idaho were investigated. The frequency of both Shiga genes and toxin was similar to that reported for Idaho cattle ( approximately 19%). ...

journal_title：Applied and environmental microbiology

authors： Gilbreath JJ,Shields MS,Smith RL,Farrell LD,Sheridan PP,Spiegel KM

更新日期：2009-02-01 00:00:00

abstract：:Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chicken...

journal_title：Applied and environmental microbiology

authors： Nothaft H,Perez-Muñoz ME,Gouveia GJ,Duar RM,Wanford JJ,Lango-Scholey L,Panagos CG,Srithayakumar V,Plastow GS,Coros C,Bayliss CD,Edison AS,Walter J,Szymanski CM

更新日期：2017-11-16 00:00:00

abstract：:A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands co...

journal_title：Applied and environmental microbiology

authors： Royer JC,Nakas JP

更新日期：1990-06-01 00:00:00

abstract：:Efficient enrichment of staphylococcal cells displaying specific heterologous affinity ligands on their cell surfaces was demonstrated by using fluorescence-activated cell sorting. Using bacterial surface display of peptide or protein libraries for the purpose of combinatorial protein engineering has previously been i...

journal_title：Applied and environmental microbiology

authors： Wernérus H,Samuelson P,Ståhl S

更新日期：2003-09-01 00:00:00

abstract：:Listeria app, isolated from 13 of 129 (10%) corn silage samples, 21 of 76 (28%) hay silage samples, and 3 of 5 (60%) grass silage samples during a previous Vermont survey were subjected to automated ribotype (RT) analysis. The 13 positive corn silage samples contained 3 Listeria monocytogenes isolated (three RTs, incl...

journal_title：Applied and environmental microbiology

authors： Ryser ET,Arimi SM,Donnelly CW

更新日期：1997-09-01 00:00:00

abstract：:The pathway of electrons required for the reduction of sulfate in sulfate-reducing bacteria (SRB) is not yet fully characterized. In order to determine the role of a transmembrane protein complex suggested to be involved in this process, a deletion in Desulfovibrio vulgaris Hildenborough was created by marker exchange...

journal_title：Applied and environmental microbiology

authors： Zane GM,Yen HC,Wall JD

更新日期：2010-08-01 00:00:00

abstract：:Thermoanaerobacter sp. strain X514 has great potential in biotechnology due to its capacity to ferment a range of C(5) and C(6) sugars to ethanol and other metabolites under thermophilic conditions. This study investigated the central metabolism of strain X514 via (13)C-labeled tracer experiments using either glucose ...

journal_title：Applied and environmental microbiology

authors： Feng X,Mouttaki H,Lin L,Huang R,Wu B,Hemme CL,He Z,Zhang B,Hicks LM,Xu J,Zhou J,Tang YJ

更新日期：2009-08-01 00:00:00

abstract：:Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO2 (n-TiO2) on the average thicknesses of Bacillus subtilis biofilm and on bacterial attachment on different surfaces were i...

journal_title：Applied and environmental microbiology

authors： Zhang P,Guo JS,Yan P,Chen YP,Wang W,Dai YZ,Fang F,Wang GX,Shen Y

更新日期：2018-04-16 00:00:00

abstract：:The effective flux between phospholipids and neutral lipids is critical for a high level of biosynthesis and accumulation of very-long-chain polyunsaturated fatty acids (VLCPUFAs), such as arachidonic acid (ARA; 20:4n-6), eicosapentaenoic acid (EPA; 20:5n-3), and docosahexaenoic acid (DHA; 22:6n-3). Here we describe a...

journal_title：Applied and environmental microbiology

authors： Chen Y,Chi HY,Meesapyodsuk D,Qiu X

更新日期：2013-03-01 00:00:00

abstract：:The frequency of plasmids in chemically stressed bacterial populations was investigated by individually adding various concentration of kanamycin, ampicillin, and mercuric chloride to soil samples. Viable bacterial populations were enumerated, soil respiration was monitored for up to 6 weeks as an indicator of physiol...

journal_title：Applied and environmental microbiology

authors： Wickham GS,Atlas RM

更新日期：1988-09-01 00:00:00

abstract：:Strains of Ascochyta rabiei which are pathogenic to chickpea (Cicer arietinum L.) readily catabolized the main chickpea isoflavone biochanin A (5,7-dihydroxy-4'-methoxyisoflavone). 3'-Hydroxylation and O-demethylation reactions led to the isoflavones pratensein, genistein, and orobol, which were rapidly further degrad...

journal_title：Applied and environmental microbiology

authors： Kraft B,Barz W

更新日期：1985-07-01 00:00:00

abstract：:Here, we report simultaneous surface profile measurements of several bacterial species involved in microbially influenced corrosion and their solid-surface interfaces by using vertical scanning interferometry. The capacity to nondestructively quantify microscale topographic changes beneath a single bacterium without i...

journal_title：Applied and environmental microbiology

authors： Waters MS,El-Naggar MY,Hsu L,Sturm CA,Luttge A,Udwadia FE,Cvitkovitch DG,Goodman SD,Nealson KH

更新日期：2009-03-01 00:00:00

abstract：:Expression of genes encoding polyamine transporters from plasmids and polyamine supplements increased furfural tolerance (growth and ethanol production) in ethanologenic Escherichia coli LY180 (in AM1 mineral salts medium containing xylose). This represents a new approach to increase furfural tolerance and may be usef...

journal_title：Applied and environmental microbiology

authors： Geddes RD,Wang X,Yomano LP,Miller EN,Zheng H,Shanmugam KT,Ingram LO

更新日期：2014-10-01 00:00:00

abstract：:Surfaces made of copper or its alloys have strong antimicrobial properties against a wide variety of microorganisms. However, the molecular mode of action responsible for the antimicrobial efficacy of metallic copper is not known. Here, we show that dry copper surfaces inactivate Candida albicans and Saccharomyces cer...

journal_title：Applied and environmental microbiology

authors： Quaranta D,Krans T,Espírito Santo C,Elowsky CG,Domaille DW,Chang CJ,Grass G

更新日期：2011-01-01 00:00:00

abstract：:In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...

journal_title：Applied and environmental microbiology

authors： Heller LC,Davis CR,Peak KK,Wingfield D,Cannons AC,Amuso PT,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides kill...

journal_title：Applied and environmental microbiology

authors： Ohsawa M,Tanaka M,Moriyama K,Shimazu M,Asano S,Miyamoto K,Haginoya K,Mitsui T,Kouya T,Taniguchi M,Hori H

更新日期：2012-07-01 00:00:00

abstract：:Genetic optimizations to achieve high-level production of three different proteins of medical importance for humans, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha 2b (IFN-alpha2b), and single-chain antibody variable fragment (scFv-phOx), were investigated during high-cell-density cultivat...

journal_title：Applied and environmental microbiology

authors： Sletta H,Tøndervik A,Hakvåg S,Aune TE,Nedal A,Aune R,Evensen G,Valla S,Ellingsen TE,Brautaset T

更新日期：2007-02-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis that grows to a higher cell density (SE-HCD) than wild type while retaining O-chain lipopolysaccharide was isolated by transforming wild type serovar Enteritidis with the cell density sensor plasmid pSB402 and selecting for bioluminescence. A luminescent strain, SE-HCD, that emi...

journal_title：Applied and environmental microbiology

authors： Guard-Petter J

更新日期：1998-06-01 00:00:00

abstract：:Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...

journal_title：Applied and environmental microbiology

authors： Olsson-Francis K,de la Torre R,Cockell CS

更新日期：2010-04-01 00:00:00

abstract：:Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the...

journal_title：Applied and environmental microbiology

authors： Monecke S,Mariani-Kurkdjian P,Bingen E,Weill FX,Balière C,Slickers P,Ehricht R

更新日期：2011-12-01 00:00:00

abstract：:We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydro...

journal_title：Applied and environmental microbiology

authors： Prado Acosta M,Mercedes Palomino M,Allievi MC,Sanchez Rivas C,Ruzal SM

更新日期：2008-12-01 00:00:00

abstract：:The growth of Vibrio parahaemolyticus in a liquid medium was compared with that of human fecal flora and estuarine flora. No marked differences were noted between growth at 25 and 37 degrees C for V. parahaemolyticus. However, the marine organisms were strongly inhibited when incubated at 37 degrees C. Incubation for ...

journal_title：Applied and environmental microbiology

authors： Dupray E,Cormier M

更新日期：1983-11-01 00:00:00

abstract：:Organic acids derived from engineered microbes can replace fossil-derived chemicals in many applications. Fungal hosts are preferred for organic acid production because they tolerate lignocellulosic hydrolysates and low pH, allowing economic production and recovery of the free acid. However, cell death caused by cytos...

journal_title：Applied and environmental microbiology

authors： Zdraljevic S,Wagner D,Cheng K,Ruohonen L,Jäntti J,Penttilä M,Resnekov O,Pesce CG

更新日期：2013-12-01 00:00:00

abstract：:The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

journal_title：Applied and environmental microbiology

authors： Sugio T,White KJ,Shute E,Choate D,Blake RC

更新日期：1992-01-01 00:00:00

abstract：:Wild-type Bacillus subtilis ferments 20 g/liter glucose in 48 h, producing lactate and butanediol, but not ethanol or acetate. To construct an ethanologenic B. subtilis strain, homologous recombination was used to disrupt the native lactate dehydrogenase (LDH) gene (ldh) by chromosomal insertion of the Zymomonas mobil...

journal_title：Applied and environmental microbiology

authors： Romero S,Merino E,Bolívar F,Gosset G,Martinez A

更新日期：2007-08-01 00:00:00