Microorganisms associated with uranium bioremediation in a high-salinity subsurface sediment.


:Although stimulation of dissimilatory metal reduction to promote the reductive precipitation of uranium has been shown to successfully remove uranium from some aquifer sediments, the organisms in the family Geobacteraceae that have been found to be associated with metal reduction in previous studies are not known to grow at the high salinities found in some uranium-contaminated groundwaters. Studies with a highly saline uranium-contaminated aquifer sediment demonstrated that the addition of acetate could stimulate the removal of U(VI) from the groundwater. This removal was associated with an enrichment in microorganisms most closely related to Pseudomonas and Desulfosporosinus species.


Appl Environ Microbiol


Nevin KP,Finneran KT,Lovley DR





Has Abstract


2003-06-01 00:00:00












  • Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli.

    abstract::The cyanophycin (CGP) synthetase gene (cphANE1) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum, which exhibits a CGP-leaky phenotype during diazotrophical growth, was cloned and expressed in Escherichia coli strain TOP10. Its amino acid sequence exhibited high similarities to CphA...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hai T,Frey KM,Steinbüchel A

    更新日期:2006-12-01 00:00:00

  • Copper-resistant enteric bacteria from United Kingdom and Australian piggeries.

    abstract::Thirty-three enteric isolates from Australian (Escherichia coli only) and United Kingdom (U.K.) (Salmonella sp., Citrobacter spp., and E. coli) piggeries were characterized with respect to their copper resistance. The copper resistance phenotypes of four new Australian E. coli isolates were comparable with that of the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Williams JR,Morgan AG,Rouch DA,Brown NL,Lee BT

    更新日期:1993-08-01 00:00:00

  • Comprehensive census of bacteria in clean rooms by using DNA microarray and cloning methods.

    abstract::A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: La Duc MT,Osman S,Vaishampayan P,Piceno Y,Andersen G,Spry JA,Venkateswaran K

    更新日期:2009-10-01 00:00:00

  • Microbial fouling of reverse-osmosis membranes used in advanced wastewater treatment technology: chemical, bacteriological, and ultrastructural analyses.

    abstract::Biofouling of reverse-osmosis membranes was investigated at an advanced wastewater treatment facility. Cellulose diacetate membranes operated for approximately 4,000 h became uniformly coated with a mucilaginous fouling layer. The fouling material was approximately 93% water by weight, and nearly 90% of the dehydrated...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ridgway HF,Kelly A,Justice C,Olson BH

    更新日期:1983-03-01 00:00:00

  • Association of azospirillum with grass roots.

    abstract::The association between grass roots and Azospirillum brasilense Sp 7 was investigated by the Fahraeus slide technique, using nitrogen-free medium. Young inoculated roots of pearl millet and guinea grass produced more mucilaginous sheath (mucigel), root hairs, and lateral roots than did uninoculated sterile controls. T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Umali-Garcia M,Hubbell DH,Gaskins MH,Dazzo FB

    更新日期:1980-01-01 00:00:00

  • Characterization of a nitrogen-regulated protein identified by cell surface biotinylation of a marine phytoplankton.

    abstract::The biotinylating reagent succinimidyl 6-(biotinamido)hexanoate was used to label the cell surfaces of the cosmopolitan, marine, eukaryotic microorganism Emiliania huxleyi under different growth conditions. Proteins characteristic of different nutrient conditions could be identified. In particular, a nitrogen-regulate...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Palenik B,Koke JA

    更新日期:1995-09-01 00:00:00

  • Characterization of a foldase, protein disulfide isomerase A, in the protein secretory pathway of Aspergillus niger.

    abstract::Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ngiam C,Jeenes DJ,Punt PJ,Van Den Hondel CA,Archer DB

    更新日期:2000-02-01 00:00:00

  • Molecular indicators used in the development of predictive models for microbial source tracking.

    abstract::A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ballesté E,Bonjoch X,Belanche LA,Blanch AR

    更新日期:2010-03-01 00:00:00

  • Identification of the functionally active methanotroph population in a peat soil microcosm by stable-isotope probing.

    abstract::The active population of low-affinity methanotrophs in a peat soil microcosm was characterized by stable-isotope probing. "Heavy" (13)C-labeled DNA, produced after microbial growth on (13)CH(4), was separated from naturally abundant (12)C-DNA by cesium chloride density gradient centrifugation and used as a template fo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Morris SA,Radajewski S,Willison TW,Murrell JC

    更新日期:2002-03-01 00:00:00

  • Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae.

    abstract::Haa1 is a transcription factor that adapts Saccharomyces cerevisiae cells to weak organic acid stresses by activating the expression of various genes. Many of these genes encode membrane proteins, such as TPO2 and YRO2 How Haa1 is activated by weak acids is not clear. Here, we show that casein kinase I isoform Hrr25 i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Collins ME,Black JJ,Liu Z

    更新日期:2017-06-16 00:00:00

  • Horizontal variation of bacterioplankton in the baltic sea.

    abstract::Variations in the thymidine incorporation rate, bacterial abundance, and mean cell volumes in the surface water (0.5 m) of the Baltic Sea in spring and summer were compared in studies with different spatial scales (570 nautical miles [nmi] [ca. 1056 km], 220 nmi [ca. 407 km], 24 nmi [ca. 44 km], 12 nmi [ca. 22 km], an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Heinänen A,Kuparinen J

    更新日期:1991-11-01 00:00:00

  • BTI1, an azoreductase with pH-dependent substrate specificity.

    abstract::The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Johansson HE,Johansson MK,Wong AC,Armstrong ES,Peterson EJ,Grant RE,Roy MA,Reddington MV,Cook RM

    更新日期:2011-06-01 00:00:00

  • Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli.

    abstract::Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold inc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Millard CS,Chao YP,Liao JC,Donnelly MI

    更新日期:1996-05-01 00:00:00

  • Evidence Regarding the UV Sunscreen Role of a Mycosporine-Like Compound in the Cyanobacterium Gloeocapsa sp.

    abstract::The UV sunscreen role commonly ascribed to mycosporine-like amino acids (MAAs) was investigated with an isolate of the terrestrial cyanobacterium Gloeocapsa sp. strain C-90-Cal-G.(2), which accumulates intracellularly an MAA with absorbance maximum at 326 nm but produces no extracellular sunscreen compound (i.e., scyt...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Garcia-Pichel F,Wingard CE,Castenholz RW

    更新日期:1993-01-01 00:00:00

  • In Situ Hydrogen Dynamics in a Hot Spring Microbial Mat during a Diel Cycle.

    abstract:UNLABELLED:Microbes can produce molecular hydrogen (H2) via fermentation, dinitrogen fixation, or direct photolysis, yet the H2 dynamics in cyanobacterial communities has only been explored in a few natural systems and mostly in the laboratory. In this study, we investigated the diel in situ H2 dynamics in a hot spring...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Revsbech NP,Trampe E,Lichtenberg M,Ward DM,Kühl M

    更新日期:2016-06-30 00:00:00

  • Conditions for growing Mycoplasma canadense and Mycoplasma verecundum in a serum-free medium.

    abstract::Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Muñoz G,Sotomayor P

    更新日期:1990-07-01 00:00:00

  • Involvement of acyl coenzyme A oxidase isozymes in biotransformation of methyl ricinoleate into gamma-decalactone by Yarrowia lipolytica.

    abstract::We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lacto...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Waché Y,Laroche C,Bergmark K,Møller-Andersen C,Aguedo M,Le Dall MT,Wang H,Nicaud JM,Belin JM

    更新日期:2000-03-01 00:00:00

  • Multiplex reverse transcription-PCR for simultaneous detection of beet necrotic yellow vein virus, Beet soilborne virus, and Beet virus Q and their vector Polymyxa betae KESKIN on sugar beet.

    abstract::Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Meunier A,Schmit JF,Stas A,Kutluk N,Bragard C

    更新日期:2003-04-01 00:00:00

  • Chromosomal location plays a role in regulation of aflatoxin gene expression in Aspergillus parasiticus.

    abstract::The nor-1 gene in the filamentous fungus Aspergillus parasiticus encodes a ketoreductase involved in aflatoxin biosynthesis. To study environmental influences on nor-1 expression, we generated plasmid pAPGUSNNB containing a nor-1 promoter-beta-glucuronidase (GUS) (encoded by uidA) reporter fusion with niaD (encodes ni...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Chiou CH,Miller M,Wilson DL,Trail F,Linz JE

    更新日期:2002-01-01 00:00:00

  • Detection of Clostridium proteoclasticum and closely related strains in the rumen by competitive PCR.

    abstract::A competitive PCR technique was used to enumerate the proteolytic bacterium Clostridium proteoclasticum from the rumen. A PCR primer, which circumscribes this organism and several closely related strains, was designed for a variable region within their 16S rRNA genes and was used in conjunction with a universal forwar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Reilly K,Attwood GT

    更新日期:1998-03-01 00:00:00

  • Production of penicillic acid and patulin by an atypical Penicillium roqueforti isolate.

    abstract::Simultaneous production of penicillic acid and patulin by an atypical strain of Penicillium roqueforti isolated from cheddar cheese is reported. Mycotoxin production was confirmed by thin-layer and gas-liquid chromatography and by ultraviolet, infrared, and mass spectral analyses. Culture extracts were toxic to Bacill...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Olivigni FJ,Bullerman LB

    更新日期:1978-02-01 00:00:00

  • Inhibition of Listeria innocua in cheddar cheese by addition of nisin Z in liposomes or by in situ production in mixed culture.

    abstract::The effect of addition of purified nisin Z in liposomes to cheese milk and of in situ production of nisin Z by Lactococcus lactis subsp. lactis biovar diacetylactis UL719 in the mixed starter on the inhibition of Listeria innocua in cheddar cheese was evaluated during 6 months of ripening. A cheese mixed starter cultu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Benech RO,Kheadr EE,Laridi R,Lacroix C,Fliss I

    更新日期:2002-08-01 00:00:00

  • Expression of rhizobial nitrogenase: influence of plant cell-conditioned medium.

    abstract::Conditioned medium was obtained from suspension cultures of soybean (Glycine max L. Merrit) cells after incubating them for 4 to 8 days with rhizobia which were separated from the soybean cells by two dialysis bags, one within another. This conditioned medium from the plant cell side (PCM) of the two membranes was use...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bednarski MA,Reporter M

    更新日期:1978-07-01 00:00:00

  • The nuclear ribosomal DNA intergenic spacer as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on pinus root systems.

    abstract::Polymorphism of the nuclear ribosomal DNA intergenic spacer (IGS) of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was studied to evaluate whether this sequence could be used in field studies to estimate the diversity of strains forming mycorrhizas on individual Pinus pinaster root systems. This sequence w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Guidot A,Lumini E,Debaud JC,Marmeisse R

    更新日期:1999-03-01 00:00:00

  • Fate of DNA encoding hygromycin resistance after meiosis in transformed strains of Gibberella fujikuroi (Fusarium moniliforme).

    abstract::Stability of foreign DNA transformed into a novel host is an important parameter in decisions to permit the release of genetically engineered microorganisms into the environment. Meiotic instability of transformed DNA has been reported in fungi such as Ascobolus, Aspergillus, and Neurospora. We used strains of Gibbere...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Leslie JF,Dickman MB

    更新日期:1991-05-01 00:00:00

  • Novel Modifications of Nonribosomal Peptides from Brevibacillus laterosporus MG64 and Investigation of Their Mode of Action.

    abstract::Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications, including biocontrol and pharmacy. Brevibacillus spp. are a rich source of NRPs yet have received little attention. In this study, we characterize four novel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Li Z,de Vries RH,Chakraborty P,Song C,Zhao X,Scheffers DJ,Roelfes G,Kuipers OP

    更新日期:2020-11-24 00:00:00

  • Association of constitutive hyperphosphorylation of Hsf1p with a defective ethanol stress response in Saccharomyces cerevisiae sake yeast strains.

    abstract::Modern sake yeast strains, which produce high concentrations of ethanol, are unexpectedly sensitive to environmental stress during sake brewing. To reveal the underlying mechanism, we investigated a well-characterized yeast stress response mediated by a heat shock element (HSE) and heat shock transcription factor Hsf1...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Noguchi C,Watanabe D,Zhou Y,Akao T,Shimoi H

    更新日期:2012-01-01 00:00:00

  • Lepidopteran-active variable-region sequence imparts coleopteran activity in eCry3.1Ab, an engineered Bacillus thuringiensis hybrid insecticidal protein.

    abstract::A unique, coleopteran-active protein, termed eCry3.1Ab, was generated following variable-region exchange of a Bacillus thuringiensis lepidopteran-active protein, Cry1Ab, with a Cry3A region. Our results support the hypothesis that this variable-region exchange is responsible for imparting strong bioactivity against th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Walters FS,deFontes CM,Hart H,Warren GW,Chen JS

    更新日期:2010-05-01 00:00:00

  • GlcNAc De-N-Acetylase from the Hyperthermophilic Archaeon Sulfolobus solfataricus.

    abstract::Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

    更新日期:2019-01-09 00:00:00

  • Use of controlled luciferase expression to monitor chemicals affecting protein synthesis.

    abstract::In this article, we present a new bioluminescent test system for the screening of chemical compounds with an inhibitory effect on protein synthesis. The test is based on the measurement of real-time in vivo light production by Escherichia coli strains expressing different luciferase genes. The eukaryotic lucGR gene fr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lampinen J,Virta M,Karp M

    更新日期:1995-08-01 00:00:00