Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes.

abstract：:The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...

journal_title：Applied and environmental microbiology

authors： Dinoto A,Marques TM,Sakamoto K,Fukiya S,Watanabe J,Ito S,Yokota A

更新日期：2006-12-01 00:00:00

abstract：:Several transposable elements were isolated from the genome of Xanthomonas oryzae pv. oryzae. These elements and an avirulence gene isolated from X. oryzae pv. oryzae were used as hybridization probes for a collection of X. oryzae pv. oryzae strains from the Philippines. Each of the sequences was present in multiple c...

journal_title：Applied and environmental microbiology

authors： Nelson RJ,Baraoidan MR,Cruz CM,Yap IV,Leach JE,Mew TW,Leung H

更新日期：1994-09-01 00:00:00

abstract：:The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus bre...

journal_title：Applied and environmental microbiology

authors： Macfarlane S,Woodmansey EJ,Macfarlane GT

更新日期：2005-11-01 00:00:00

abstract：:Five cases of primary amoebic meningoencephalitis associated with swimming in freshwater lakes have been recorded in Florida over the past 14 years. The present study demonstrated that pathogenic Naegleria, the causative agent, is relatively widespread. Twelve of 26 lakes sampled only once yielded the amoeba. Populati...

journal_title：Applied and environmental microbiology

authors： Wellings FM,Amuso PT,Chang SL,Lewis AL

更新日期：1977-12-01 00:00:00

abstract：:Xenorhabdus nematophila engages in a mutualistic association with the nematode Steinernema carpocapsae. The nematode invades and traverses the gut of susceptible insects. X. nematophila is released in the insect blood (hemolymph), where it suppresses host immune responses and functions as a pathogen. X. nematophila pr...

journal_title：Applied and environmental microbiology

authors： Singh S,Reese JM,Casanova-Torres AM,Goodrich-Blair H,Forst S

更新日期：2014-07-01 00:00:00

abstract：:Three particulate methane monooxygenase PCR primer sets (A189-A682, A189-A650, and A189-mb661) were investigated for their ability to assess methanotroph diversity in soils from three sites, i.e., heath, oak, and sitka, each of which was capable of oxidizing atmospheric concentrations of methane. Each PCR primer set w...

journal_title：Applied and environmental microbiology

authors： Bourne DG,McDonald IR,Murrell JC

更新日期：2001-09-01 00:00:00

abstract：:In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...

journal_title：Applied and environmental microbiology

authors： Pontes MH,Dale C

更新日期：2011-03-01 00:00:00

abstract：:When carbon sources are changed, Saccharomyces cerevisiae transcriptional patterns drastically change. To identify genes whose transcription can be used to quantitatively measure sugar concentrations, we searched genomic expression databases for a set of genes that are highly induced during the diauxic shift, and we u...

journal_title：Applied and environmental microbiology

authors： Chambers P,Issaka A,Palecek SP

更新日期：2004-01-01 00:00:00

abstract：:Diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the United States, was studied to determine its potential role as a vehicle of Salmonella enterica serotype Poona, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogen...

journal_title：Applied and environmental microbiology

authors： Gibbs DS,Anderson GL,Beuchat LR,Carta LK,Williams PL

更新日期：2005-05-01 00:00:00

abstract：:Fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with beta(2-6) and beta(2-1) linkages, respectively. The probiotic bacterium Lactobacillus johnsonii strain NCC 533 possesses a single fructansucrase gene (open reading frame AAS08734) annotated as a putative levansucrase p...

journal_title：Applied and environmental microbiology

authors： Anwar MA,Kralj S,van der Maarel MJ,Dijkhuizen L

更新日期：2008-06-01 00:00:00

abstract：:Thirty-eight cultures of Legionella pneumophila isolated from surface waters were characterized by their morphological, tinctorial, biochemical, and serological properties and by their ability to produce disease in guinea pigs. Their susceptibility to antimicrobial agents also was tested. When they were compared with...

journal_title：Applied and environmental microbiology

authors： Orrison LH,Cherry WB,Fliermans CB,Dees SB,McDougal LK,Dodd DJ

更新日期：1981-07-01 00:00:00

abstract：:Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days,...

journal_title：Applied and environmental microbiology

authors： Bérubé A,Trudel M,Payment P

更新日期：1989-06-01 00:00:00

abstract：:This study examined the inactivation of human norovirus (HuNoV) GI.1 and GII.4 by chlorine under conditions mimicking sewage treatment. Using a porcine gastric mucin-magnetic bead (PGM-MB) assay, no statistically significant loss in HuNoV binding (inactivation) was observed for secondary effluent treatments of ≤25 ppm...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Fay JP,Calci K,Pouillot R,Woods J,Chen H,Niemira BA,Van Doren JM

更新日期：2017-11-16 00:00:00

abstract：:The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bul...

journal_title：Applied and environmental microbiology

authors： He R,Zeng J,Zhao D,Huang R,Yu Z,Wu QL

更新日期：2020-07-02 00:00:00

abstract：:Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many drinking water utilities in the United States now use monochloramine as a secondary disinfectant to minimize disinfectant by-product formation and biofilm growth. The inactivation of human adenoviruses 2, 40...

journal_title：Applied and environmental microbiology

authors： Cromeans TL,Kahler AM,Hill VR

更新日期：2010-02-01 00:00:00

abstract：:Disruption of the aflatoxin biosynthesis cluster gene aflY (hypA) gave Aspergillus parasiticus transformants that accumulated versicolorin A. This gene is predicted to encode the Baeyer-Villiger oxidase necessary for formation of the xanthone ring of the aflatoxin precursor demethylsterigmatocystin. ...

journal_title：Applied and environmental microbiology

authors： Ehrlich KC,Montalbano B,Boué SM,Bhatnagar D

更新日期：2005-12-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：:Pseudomonas syringae pv. phaseolicola NPS3121 hrp sequences were used as hybridization probes in a restriction fragment length polymorphism (RFLP) analysis of 24 P. syringae pv. tabaci strains as a means to evaluate the genetic and taxonomic relationship of pathovars of P. syringae. Southern blot analyses of genomic r...

journal_title：Applied and environmental microbiology

authors： Scholz BK,Jakobek JL,Lindgren PB

更新日期：1994-04-01 00:00:00

abstract：:Across evolution, N-glycosylation involves oligosaccharyltransferases that transfer lipid-linked glycans to selected Asn residues of target proteins. While these enzymes catalyze similar reactions in each domain, differences exist in terms of the chemical composition, length and degree of phosphorylation of the lipid ...

journal_title：Applied and environmental microbiology

authors： Cohen-Rosenzweig C,Guan Z,Shaanan B,Eichler J

更新日期：2014-01-01 00:00:00

abstract：:The pathogenic oomycete Aphanomyces invadans is the primary etiological agent in ulcerative mycosis, an ulcerative skin disease caused by a fungus-like agent of wild and cultured fish. We developed sensitive PCR and fluorescent peptide nucleic acid in situ hybridization (FISH) assays to detect A. invadans. Laboratory-...

journal_title：Applied and environmental microbiology

authors： Vandersea MW,Litaker RW,Yonnish B,Sosa E,Landsberg JH,Pullinger C,Moon-Butzin P,Green J,Morris JA,Kator H,Noga EJ,Tester PA

更新日期：2006-02-01 00:00:00

abstract：:Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stabil...

journal_title：Applied and environmental microbiology

authors： Teachey ME,McDonald JM,Ottesen EA

更新日期：2019-05-16 00:00:00

abstract：:In light of recent proposals that iron (Fe) availability may play an important role in controlling oceanic primary production and nutrient flux, its regulatory impact on N(2) fixation and production dynamics was investigated in the widespread and biogeochemically important diazotrophic, planktonic cyanobacteria Tricho...

journal_title：Applied and environmental microbiology

authors： Paerl HW,Prufert-Bebout LE,Guo C

更新日期：1994-03-01 00:00:00

abstract：:Binding of three Bacillus thuringiensis insecticidal crystal proteins (ICPs) to the midgut epithelium of Ostrinia nubilalis larvae was characterized by performing binding experiments with both isolated brush border membrane vesicles and gut tissue sections. Our results demonstrate that two independent ICP receptors ar...

journal_title：Applied and environmental microbiology

authors： Denolf P,Jansens S,Peferoen M,Degheele D,Van Rie J

更新日期：1993-06-01 00:00:00

abstract：:We developed an improved method for cultivation-independent sorting of bacterial cells. The technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. Due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the c...

journal_title：Applied and environmental microbiology

authors： Zwirglmaier K,Ludwig W,Schleifer KH

更新日期：2004-01-01 00:00:00

abstract：:A growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. Using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that Peptostreptococcus productus is often the most numerous urease-forming species in human feces. Also, some fecal strains ...

journal_title：Applied and environmental microbiology

authors： Wozny MA,Bryant MP,Holdeman LV,Moore WE

更新日期：1977-05-01 00:00:00

abstract：:The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with d...

journal_title：Applied and environmental microbiology

authors： Germida JJ

更新日期：1988-01-01 00:00:00

abstract：:We have analyzed the impact of surface-to-volume ratio on final bacterial concentrations after batch growth. We examined six bottle sizes (20 to 1,000 ml) using three independent enumeration methods to quantify growth. We found no evidence of a so-called volumetric bottle effect, thus contradicting numerous previous r...

journal_title：Applied and environmental microbiology

authors： Hammes F,Vital M,Egli T

更新日期：2010-02-01 00:00:00

abstract：:A restriction fragment length polymorphism analysis of nuclear ribosomal RNA genes (rDNA) was used to measure the amount and distribution of genetic variability in populations of the Japanese pear pathotype of Alternaria alternata on both micro- and macrogeographical scales. A total of 322 isolates were obtained from ...

journal_title：Applied and environmental microbiology

authors： Adachi Y,Watanabe H,Tanabe K,Doke N,Nishimura S,Tsuge T

更新日期：1993-10-01 00:00:00

abstract：:Interactions of soybean with Bradyrhizobium japonicum 123 (serogroup 123) and 138 (serogroup c1) were used to examine the relationship between early infection rates, competition for nodulation, and patterns of nodule occupancy. Both strains formed more infections in autoclaved soil (sterile soil) than in untreated soi...

journal_title：Applied and environmental microbiology

authors： Zdor RE,Pueppke SG

更新日期：1988-08-01 00:00:00

abstract：:Six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. After 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. Fifty colonies were randomly selected from each fecal ...

journal_title：Applied and environmental microbiology

authors： Moore WE,Moore LV,Cato EP,Wilkins TD,Kornegay ET

更新日期：1987-07-01 00:00:00