Molecular assays for detecting Aphanomyces invadans in ulcerative mycotic fish lesions.

abstract：:Sakacin A is an antilisterial bacteriocin produced by Lactobacillus sake Lb706. In order to identify genes involved in sakacin A production and immunity, the plasmid fraction of L. sake Lb706 was shotgun cloned directly into a sakacin A-nonproducing and -sensitive variant, L. sake Lb706-B, by using the broad-host-rang...

journal_title：Applied and environmental microbiology

authors： Axelsson L,Holck A,Birkeland SE,Aukrust T,Blom H

更新日期：1993-09-01 00:00:00

abstract：:Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

journal_title：Applied and environmental microbiology

authors： Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

更新日期：2003-02-01 00:00:00

abstract：:Bottlenecks in protein expression and secretion often limit the development of industrial processes. By manipulating chaperone and foldase levels, improvements in yeast secretion were found for a number of proteins. Recently, sustained endoplasmic reticulum stress, occurring due to recombinant protein production, was ...

journal_title：Applied and environmental microbiology

authors： Raimondi S,Zanni E,Talora C,Rossi M,Palleschi C,Uccelletti D

更新日期：2008-12-01 00:00:00

abstract：:The bacterial succession on the skin of broiler chicken carcasses stored at 2 degrees C was traced, and the ability of representative isolates to produce off-odors was determined by using sterile leg and breast muscle sections. Off-odors were identified by olfactory and chemical means. The inability of peptone-iron ag...

journal_title：Applied and environmental microbiology

authors： Daud HB,McMeekin TA,Thomas CJ

更新日期：1979-03-01 00:00:00

abstract：:Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We ...

journal_title：Applied and environmental microbiology

authors： Thar R,Kühl M,Holst G

更新日期：2001-06-01 00:00:00

abstract：:Haa1 is a transcription factor that adapts Saccharomyces cerevisiae cells to weak organic acid stresses by activating the expression of various genes. Many of these genes encode membrane proteins, such as TPO2 and YRO2 How Haa1 is activated by weak acids is not clear. Here, we show that casein kinase I isoform Hrr25 i...

journal_title：Applied and environmental microbiology

authors： Collins ME,Black JJ,Liu Z

更新日期：2017-06-16 00:00:00

abstract：:The objective of the present study was to develop a mathematical model of pathogenic bacterial inactivation kinetics in a gastric environment in order to further understand a part of the infectious dose-response mechanism. The major bacterial pathogens Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella s...

journal_title：Applied and environmental microbiology

authors： Koseki S,Mizuno Y,Sotome I

更新日期：2011-02-01 00:00:00

abstract：:Strains of Ascochyta rabiei which are pathogenic to chickpea (Cicer arietinum L.) readily catabolized the main chickpea isoflavone biochanin A (5,7-dihydroxy-4'-methoxyisoflavone). 3'-Hydroxylation and O-demethylation reactions led to the isoflavones pratensein, genistein, and orobol, which were rapidly further degrad...

journal_title：Applied and environmental microbiology

authors： Kraft B,Barz W

更新日期：1985-07-01 00:00:00

abstract：:Green mold disease (causal agent, Trichoderma) has resulted in severe crop losses on mushroom farms worldwide in recent years. We analyzed 160 isolates of Trichoderma from mushroom farms for morphological, cultural, and molecular characteristics and classified these isolates into phenotypic groups. The most common gro...

journal_title：Applied and environmental microbiology

authors： Castle A,Speranzini D,Rghei N,Alm G,Rinker D,Bissett J

更新日期：1998-01-01 00:00:00

abstract：:Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could...

journal_title：Applied and environmental microbiology

authors： Santangelo TJ,Cubonová L,Reeve JN

更新日期：2008-05-01 00:00:00

abstract：:A rare strain of Clostridium botulinum subtype Ab was isolated from a canned macrobiotic food suspected of being linked to a fatal case of food-borne botulism. The strain was recovered and identified by conventional methods modified by the inclusion of a PCR assay (G. Franciosa, J.L. Ferreira, and C.L. Hatheway, J. Cl...

journal_title：Applied and environmental microbiology

authors： Franciosa G,Fenicia L,Pourshaban M,Aureli P

更新日期：1997-03-01 00:00:00

abstract：:Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozy...

journal_title：Applied and environmental microbiology

authors： Vanden Wymelenberg A,Covert S,Cullen D

更新日期：1993-10-01 00:00:00

abstract：:An immunolatex reagent was developed from antiserum to Fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, Periplaneta americana L. A new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microsc...

journal_title：Applied and environmental microbiology

authors： Bracke JW,Markovetz AJ

更新日期：1978-01-01 00:00:00

abstract：:The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...

journal_title：Applied and environmental microbiology

authors： Nies L,Vogel TM

更新日期：1990-09-01 00:00:00

abstract：:Total coliforms and total coliforms resistant to streptomycin, tetracycline, or chloramphenicol were isolated from filtered activated sludge effluents before and after UV light irradiation. Although the UV irradiation effectively disinfected the wastewater effluent, the percentage of the total surviving coliform popul...

journal_title：Applied and environmental microbiology

authors： Meckes MC

更新日期：1982-02-01 00:00:00

abstract：:Electrophoretic karyotypes (EKs) of 83 isolates were variable within agricultural and natural populations of Sclerotinia sclerotiorum, as well as among S. sclerotiorum, Sclerotinia minor, and Sclerotinia trifoliorum. Variation in EKs was not observed within six mitotic or three meiotic lineages of isolates. EKs of 8 t...

journal_title：Applied and environmental microbiology

authors： Errampalli D,Kohn LM

更新日期：1996-11-01 00:00:00

abstract：:The metabolism of aflatoxin B1 to aflatoxins M1 and Q1 by rat liver microsomes from animals pretreated with polychlorinated or polybrominated biphenyl congeners depended on the structure of the halogenated biphenyl inducers. Microsomes from rats treated with phenobarbital (PB) or halogenated biphenyls that exhibit PB-...

journal_title：Applied and environmental microbiology

authors： Halvorson MR,Phillips TD,Safe SH,Robertson LW

更新日期：1985-04-01 00:00:00

abstract：:The diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near Comodoro Rivadavia in Patagonia (Argentina) were investigated. Community shifts during long-term incubation with diesel fuel at four salinities between 0 and 20% NaCl were monitored by single-...

journal_title：Applied and environmental microbiology

authors： Kleinsteuber S,Riis V,Fetzer I,Harms H,Müller S

更新日期：2006-05-01 00:00:00

abstract：:A simple and efficient process for the production of a ruminant feed supplement, rich in crude protein (defined as total N X 6.25), by bacterial fermentation of cheese whey has been developed. The lactose in unpasteurized whey is fermented to lactate acid by Lactobacillus bulgaricus at a temperature of 43 degrees C an...

journal_title：Applied and environmental microbiology

authors： Reddy CA,Henderson HE,Erdman MD

更新日期：1976-12-01 00:00:00

abstract：:The physiology of filamentous fungi at growth rates approaching zero has been subject to limited study and exploitation. With the aim of uncoupling product formation from growth, we have revisited and improved the retentostat cultivation method for Aspergillus niger. A new retention device was designed allowing reliab...

journal_title：Applied and environmental microbiology

authors： Jørgensen TR,Nitsche BM,Lamers GE,Arentshorst M,van den Hondel CA,Ram AF

更新日期：2010-08-01 00:00:00

abstract：:Resistance to vancomycin permitted detection, in a culture of Streptococcus cremoris 290PC, of a contaminant gram-positive coccus. Morphological and physiological characteristics indicated that this bacterium was a strain of Leuconostoc sp., designated PO184. This strain contained four plasmid species, which were dist...

journal_title：Applied and environmental microbiology

authors： Orberg PK,Sandine WE

更新日期：1984-12-01 00:00:00

abstract：:Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the opti...

journal_title：Applied and environmental microbiology

authors： Muñoz-Rojas J,Bernal P,Duque E,Godoy P,Segura A,Ramos JL

更新日期：2006-01-01 00:00:00

abstract：:Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to...

journal_title：Applied and environmental microbiology

authors： Upton ME,Fogarty WM

更新日期：1977-01-01 00:00:00

abstract：:The type III secretion system (T3SS) is a specialized secretion apparatus that is commonly used by many plant and animal pathogenic bacteria to deliver proteins, termed effectors, to the interior of the host cells. These effectors suppress host defenses and interfere with signal transduction pathways to promote infect...

journal_title：Applied and environmental microbiology

authors： Jiménez-Guerrero I,Pérez-Montaño F,Medina C,Ollero FJ,López-Baena FJ

更新日期：2017-02-15 00:00:00

abstract：:1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been consider...

journal_title：Applied and environmental microbiology

authors： Monincová M,Prokop Z,Vévodová J,Nagata Y,Damborsky J

更新日期：2007-03-01 00:00:00

abstract：:The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influenc...

journal_title：Applied and environmental microbiology

authors： Aranda-Olmedo I,Marín P,Ramos JL,Marqués S

更新日期：2006-11-01 00:00:00

abstract：:Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

journal_title：Applied and environmental microbiology

authors： Camper AK,McFeters GA

更新日期：1979-03-01 00:00:00

abstract：:The objective of this study was to determine the effect of high pressure (HP) on the inactivation of microbial contaminants in Cheddar cheese (Escherichia coli K-12, Staphylococcus aureus ATCC 6538, and Penicillium roqueforti IMI 297987). Initially, cheese slurries inoculated with E. coli, S. aureus, and P. roqueforti...

journal_title：Applied and environmental microbiology

authors： O'Reilly CE,O'Connor PM,Kelly AL,Beresford TP,Murphy PM

更新日期：2000-11-01 00:00:00

abstract：:One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector co...

journal_title：Applied and environmental microbiology

authors： Gonella E,Negri I,Marzorati M,Mandrioli M,Sacchi L,Pajoro M,Crotti E,Rizzi A,Clementi E,Tedeschi R,Bandi C,Alma A,Daffonchio D

更新日期：2011-02-01 00:00:00

abstract：:DysI is identified as the protein that confers specific immunity to dysgalacticin, a plasmid-encoded streptococcal bacteriocin. dysI is transcribed as part of the copG-repB-dysI replication-associated operon. DysI appears to function at the membrane level to prevent the inhibitory effects of dysgalacticin on glucose t...

journal_title：Applied and environmental microbiology

authors： Swe PM,Heng NC,Cook GM,Tagg JR,Jack RW

更新日期：2010-12-01 00:00:00