Nuclear Ribosomal DNA as a Probe for Genetic Variability in the Japanese Pear Pathotype of Alternaria alternata.

abstract：:Cells of Bradyrhizobium japonicum were grown in media containing either 1.0 mM or 0.5 muM phosphorus. In growth pouch experiments, infection of the primary root of soybean (Glycine max (L.) Merr.) by B. japonicum USDA 31, 110, and 142 was significantly delayed when P-limited cells were applied to the root. In a greenh...

journal_title：Applied and environmental microbiology

authors： Mullen MD,Israel DW,Wollum AG

更新日期：1988-10-01 00:00:00

abstract：:A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-microm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-microm-pore-size filters were affiliated with the candidate divisions O...

journal_title：Applied and environmental microbiology

authors： Miyoshi T,Iwatsuki T,Naganuma T

更新日期：2005-02-01 00:00:00

abstract：:Several investigations have identified benzothiophene-2,3-dione in the organic solvent extracts of acidified cultures degrading dibenzothiophene via the Kodama pathway. In solution at neutral pH, the 2,3-dione exists as 2-mercaptophenylglyoxylate, which cyclizes upon acidification and is extracted as the 2,3-dione. Th...

journal_title：Applied and environmental microbiology

authors： Bressler DC,Fedorak PM

更新日期：2001-11-01 00:00:00

abstract：:Pure cultures of Geobacter sulfurreducens and other Fe(III)-reducing bacteria accumulated hydrogen to partial pressures of 5 to 70 Pa with acetate, butyrate, benzoate, ethanol, lactate, or glucose as the electron donor if electron release to an acceptor was limiting. G. sulfurreducens coupled acetate oxidation with el...

journal_title：Applied and environmental microbiology

authors： Cord-Ruwisch R,Lovley DR,Schink B

更新日期：1998-06-01 00:00:00

abstract：:Xenorhabdus nematophila engages in a mutualistic association with the nematode Steinernema carpocapsae. The nematode invades and traverses the gut of susceptible insects. X. nematophila is released in the insect blood (hemolymph), where it suppresses host immune responses and functions as a pathogen. X. nematophila pr...

journal_title：Applied and environmental microbiology

authors： Singh S,Reese JM,Casanova-Torres AM,Goodrich-Blair H,Forst S

更新日期：2014-07-01 00:00:00

abstract：:The effects of pulsed electric field (PEF) treatment and processing factors on the inactivation kinetics of Listeria innocua NCTC 11289 were investigated by using a pilot plant PEF unit with a flow rate of 200 liters/h. The electric field strength, pulse length, number of pulses, and inlet temperature were the most si...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Dutreux N,Smelt JP,Lelieveld HL

更新日期：1999-12-01 00:00:00

abstract：:A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiologi...

journal_title：Applied and environmental microbiology

authors： Payne MS,Hall MR,Sly L,Bourne DG

更新日期：2007-03-01 00:00:00

abstract：:Depending on the moment of cellobiose starvation, Clostridium cellulolyticum cells behave in different ways. Cells starved during the exponential phase of growth sporulate at 30%, whereas exhaustion of the carbon substrate at the beginning of growth does not provoke cell sporulation. Growth in the presence of excess c...

journal_title：Applied and environmental microbiology

authors： Gehin A,Gelhaye E,Raval G,Petitdemange H

更新日期：1995-03-01 00:00:00

abstract：:The moderately halophilic bacterium Halobacillus halophilus carries a homologue of LuxS, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxS of H. halophilus is part of an operon that encodes an S-adenosylmethionine-dependent...

journal_title：Applied and environmental microbiology

authors： Sewald X,Saum SH,Palm P,Pfeiffer F,Oesterhelt D,Müller V

更新日期：2007-01-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:We report the spontaneous formation of a stable mannitol-producing variant of Leuconostoc pseudomesenteroides. The mannitol-producing variant showed mannitol dehydrogenase activity which was absent in the parental strain. It was also able to use fructose and glucose simultaneously, whereas the parental strain showed d...

journal_title：Applied and environmental microbiology

authors： Grobben GJ,Peters SW,Wisselink HW,Weusthuis RA,Hoefnagel MH,Hugenholtz J,Eggink G

更新日期：2001-06-01 00:00:00

abstract：:The insertion sequence IS629, which is highly prevalent in Escherichia coli O157:H7 genomes, was found to be absent in O157:H- strains, which are on a divergent pathway in the emergence of O157:H7. Although O157:H- is deficient in IS629, it permits IS629 transposition, with an excision frequency higher than that of an...

journal_title：Applied and environmental microbiology

authors： Rump LV,Fischer M,González-Escalona N

更新日期：2011-07-01 00:00:00

abstract：:Metabolic engineering and multisubunit protein production necessitate the expression of multiple genes at coordinated levels. In bacteria, genes for multisubunit proteins or metabolic pathways are often expressed in operons under the control of a single promoter; expression of the genes is coordinated by varying trans...

journal_title：Applied and environmental microbiology

authors： Smolke CD,Carrier TA,Keasling JD

更新日期：2000-12-01 00:00:00

abstract：:Some nitrate- and Fe(III)-reducing microorganisms are capable of oxidizing Fe(II) with nitrate as the electron acceptor. This enzymatic pathway may facilitate the development of anaerobic microbial communities that take advantage of the energy available during Fe-N redox oscillations. We examined this phenomenon in sy...

journal_title：Applied and environmental microbiology

authors： Coby AJ,Picardal F,Shelobolina E,Xu H,Roden EE

更新日期：2011-09-01 00:00:00

abstract：:Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

更新日期：2002-02-01 00:00:00

abstract：:Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and o...

journal_title：Applied and environmental microbiology

authors： Zhou Z,Raskin L,Zilles JL

更新日期：2010-04-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00

abstract：:Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We ...

journal_title：Applied and environmental microbiology

authors： Thar R,Kühl M,Holst G

更新日期：2001-06-01 00:00:00

abstract：UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...

journal_title：Applied and environmental microbiology

authors： Figueroa-Cuilan W,Daniel JJ,Howell M,Sulaiman A,Brown PJ

更新日期：2016-07-29 00:00:00

abstract：:A molecular analysis of betaproteobacterial ammonia oxidizers and a N(2)O isotopomer analysis were conducted to study the sources of N(2)O emissions during the cow manure composting process. Much NO(2)(-)-N and NO(3)(-)-N and the Nitrosomonas europaea-like amoA gene were detected at the surface, especially at the top ...

journal_title：Applied and environmental microbiology

authors： Maeda K,Toyoda S,Shimojima R,Osada T,Hanajima D,Morioka R,Yoshida N

更新日期：2010-03-01 00:00:00

abstract：:The composition of phospholipids from Mycobacterium convolutum R22 was determined after growth at two temperatures (20 and 30 degrees C) with 1-chlorohexadecane as the substrate. Comparisons were made with the phospholipids of cells grown on n-hexadecane. Phosphatidylinositolmannosides and phosphatidylethanolamine (PE...

journal_title：Applied and environmental microbiology

authors： Murphy GL,Perry JJ

更新日期：1987-01-01 00:00:00

abstract：:Trench leachate samples collected anoxically from shallow-land, low-level radioactive waste disposal sites were analyzed for total aerobic and anaerobic populations, sulfate reducers, denitrifiers, and methanogens. Among the several aerobic and anaerobic bacteria isolated, only Bacillus sp., Pseudomonas sp., Citrobact...

journal_title：Applied and environmental microbiology

authors： Francis AJ,Dobbs S,Nine BJ

更新日期：1980-07-01 00:00:00

abstract：:Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...

journal_title：Applied and environmental microbiology

authors： Draggan S

更新日期：1976-02-01 00:00:00

abstract：:Evaluating different swabbing materials for spore recovery efficiency (RE) from steel surfaces, we recorded the maximum RE (71%) of 10(7) Bacillus subtilis spores with Tulips cotton buds, followed by Johnson's cotton buds and standard Hi-Media cotton, polyester, nylon, and foam (23%) swabs. Among cotton swabs, instant...

journal_title：Applied and environmental microbiology

authors： Thomas P,Mujawar MM,Upreti R,Sekhar AC

更新日期：2013-01-01 00:00:00

abstract：:A predictive and feedback proportional control algorithm, developed for fed-batch fermentations and described in a companion paper (G. L. Kleman, J. J. Chalmers, G. W. Luli, and W. R. Strohl, Appl. Environ. Microbiol. 57:910-917, 1991), was used in this work to control a continuous culture on the basis of the soluble-...

journal_title：Applied and environmental microbiology

authors： Kleman GL,Chalmers JJ,Luli GW,Strohl WR

更新日期：1991-04-01 00:00:00

abstract：:To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three s...

journal_title：Applied and environmental microbiology

authors： Van Rie J,Jansens S,Höfte H,Degheele D,Van Mellaert H

更新日期：1990-05-01 00:00:00

abstract：:Identification and classification of Vibrio species have relied upon band pattern methods (e.g., amplified fragment length polymorphism) and DNA-DNA hybridization. However, data generated by these methods cannot be used to build an online electronic taxonomy. In order to overcome these limitations, we developed the fi...

journal_title：Applied and environmental microbiology

authors： Thompson FL,Gomez-Gil B,Vasconcelos AT,Sawabe T

更新日期：2007-07-01 00:00:00

abstract：:Coumarin concentrations that inhibited growth of Pythium sp. rapidly decreased the rate of incorporation of [14C]glucose into the mycelium. Coumarin also reduced drastically the carbohydrate and protein content of the cytoplasm and, to a lesser extent, the amino acid content and cell wall fraction. Enzymes related to ...

journal_title：Applied and environmental microbiology

authors： Neto FC,Dietrich SM

更新日期：1977-09-01 00:00:00

abstract：:The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed t...

journal_title：Applied and environmental microbiology

authors： Lemaître JP,Duroux A,Pimpie R,Duez JM,Milat ML

更新日期：2015-03-01 00:00:00

abstract：:The plant-pathogenic fungus Fusarium oxysporum was successfully transformed with the beta-D-glucuronidase gene from Escherichia coli (gusA) (GUS system) in combination with the gene for nitrate reductase (niaD) as the selectable marker. The frequency of cotransformation, as determined by GUS expression on plates conta...

journal_title：Applied and environmental microbiology

authors： Couteaudier Y,Daboussi MJ,Eparvier A,Langin T,Orcival J

更新日期：1993-06-01 00:00:00