Retrieval of concentrated and undecompressed microbial populations from the deep sea.

Abstract:

:A device for sampling at depths of up to 6,000 m is described in which 3 liters of seawater is concentrated over a Nucleopore filter to about 13 ml and retrieved under in situ pressure and temperature. Subsamples can be withdrawn into transfer units that are equipped with individual gas accumulators for preventing loss of pressure during prolonged periods of storage. Transfer of samples or sample portions into sterile medium contained in pre-pressurized incubation vessels and continued subsampling therefrom permit time course experiments for the study of natural populations of deep-sea microorganisms in the absence of decompression. A test experiment with a water sample from a depth of 2,600 m supplemented with radioactively labeled Casamino Acids showed reduced rates of substrate incorporation and respiration as compared with data from a decompressed control. The barotolerance observed in this study was characterized by reduced, rather than equal, activities recorded at elevated pressures as compared with 1-atm controls.

journal_name

Appl Environ Microbiol

authors

Jannasch HW,Wirsen CO

doi

10.1128/AEM.33.3.642-646.1977

keywords:

subject

Has Abstract

pub_date

1977-03-01 00:00:00

pages

642-6

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

33

pub_type

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