Role of Pili (Fimbriae) in Attachment of Bradyrhizobium japonicum to Soybean Roots.

Abstract:

:Pili (fimbriae) were observed on cells of each of the five strains of Bradyrhizobium japonicum and the one strain of Rhizobium trifolii examined. Pili on B. japonicum were about 4 nm in diameter and polarly expressed. Piliated cells were estimated by transmission electron microscopy and hydrophobic attachment to polystyrene to constitute only a small percentage of the total population. The proportion of piliated cells in these populations was dependent on culture age in some strains. Piliated B. japonicum cells were selectively and quantitatively removed from suspension when cultures were incubated with either soybean roots or hydrophobic plastic surfaces, indicating that pili were involved in the attachment of the bacteria to these surfaces. Pili from B. japonicum 110 ARS were purified and found to have a subunit molecular weight of approximately 21,000. Treatment of B. japonicum suspensions with antiserum against the isolated pili reduced attachment to soybean roots by about 90% and nodulation by about 80%. Pili appear to be important mediators of attachment of B. japonicum to soybean roots under the conditions examined.

journal_name

Appl Environ Microbiol

authors

Vesper SJ,Bauer WD

doi

10.1128/AEM.52.1.134-141.1986

keywords:

subject

Has Abstract

pub_date

1986-07-01 00:00:00

pages

134-41

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

52

pub_type

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