Abstract:
:Eighteen strains of Agrobacterium tumefaciens isolated from crown galls were tested for agrocin production. Of six agrocin-producing strains, one (D286) produced a broad-host-range agrocin active against strains carrying nopaline, octopine, and agropine type Ti plasmids. Sensitivity to agrocin D286 was found to map in the 11- to 18-megadalton region of the nopaline Ti plasmid pTiC58. The agrocin was partially purified, and its physical characteristics were consistent with its being a nucleotide, as is agrocin 84. Agrocin D286 was shown to inhibit DNA, RNA, and protein syntheses. Strain D286 spontaneously lost its pathogenicity, and its potential for use in the biological control of crown gall is discussed.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Hendson M,Askjaer L,Thomson JA,van Montagu Mdoi
10.1128/AEM.45.5.1526-1532.1983keywords:
subject
Has Abstractpub_date
1983-05-01 00:00:00pages
1526-32issue
5eissn
0099-2240issn
1098-5336journal_volume
45pub_type
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