Role of the bga1-encoded extracellular {beta}-galactosidase of Hypocrea jecorina in cellulase induction by lactose.

abstract：:A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 ...

journal_title：Applied and environmental microbiology

authors： van de Guchte M,van der Vossen JM,Kok J,Venema G

更新日期：1989-01-01 00:00:00

abstract：:Secondary sewage effluent containing about 3 X 10(4) plaque-forming units of polio virus type 1 (LSc) per ml was passed through columns 250 cm in length packed with calcareous sand from an area in the Salt River bed used for ground-water recharge of secondary sewage effluent. Viruses were not detected in 1-ml samples ...

journal_title：Applied and environmental microbiology

authors： Lance JC,Gerba CP,Melnick JL

更新日期：1976-10-01 00:00:00

abstract：:Vibrio cholerae O1 strain TSI-4 (El Tor, Ogawa) can shift to a rugose colony morphology from its normal translucent colony morphology in response to nutrient starvation. We have investigated differences between the rugose and translucent forms of V. cholerae O1 strain TSI-4. Electron microscopic examination of the rug...

journal_title：Applied and environmental microbiology

authors： Wai SN,Mizunoe Y,Takade A,Kawabata SI,Yoshida SI

更新日期：1998-10-01 00:00:00

abstract：:Microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by Curvularia lunata (NRRL 2178). The fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its str...

journal_title：Applied and environmental microbiology

authors： Otten S,Rosazza JP

更新日期：1979-08-01 00:00:00

abstract：:Bacterial growth in biofilms is the major cause of recalcitrant biofouling in industrial processes and of persistent infections in clinical settings. The use of bacteriophage treatment to lyse bacteria in biofilms has attracted growing interest. In particular, many natural or engineered phages produce depolymerases to...

journal_title：Applied and environmental microbiology

authors： Pei R,Lamas-Samanamud GR

更新日期：2014-09-01 00:00:00

abstract：:The effect of autoproteolysis of Lactococcus lactis lactocepin III on its specificity towards beta-casein was investigated. beta-Casein degradation was performed by using either an autolysin-defective derivative of L. lactis MG1363 carrying the proteinase genes of L. lactis SK11, which was unable to transport oligopep...

journal_title：Applied and environmental microbiology

authors： Flambard B,Juillard V

更新日期：2000-12-01 00:00:00

abstract：:To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a hig...

journal_title：Applied and environmental microbiology

authors： Yan J,Han XZ,Ji ZJ,Li Y,Wang ET,Xie ZH,Chen WF

更新日期：2014-09-01 00:00:00

abstract：:Nucleic acid-based analytical methods, ranging from species-targeted PCRs to metagenomics, have greatly expanded our understanding of microbiological diversity in natural samples. However, these methods provide only limited information on the activities and physiological states of microorganisms in samples. Even the m...

journal_title：Applied and environmental microbiology

authors： Cangelosi GA,Meschke JS

更新日期：2014-10-01 00:00:00

abstract：:A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was al...

journal_title：Applied and environmental microbiology

authors： Heylen K,Ettwig K,Hu Z,Jetten M,Kartal B

更新日期：2012-04-01 00:00:00

abstract：:Of more than 400 bacteria isolated from turbot (Scophthalmus maximus), 89 have previously been shown to inhibit the in vitro growth of the fish pathogen Vibrio anguillarum. The aim of the present study was to investigate the potential of seven of these strains, as well as of intestinal isolates (four strains) from a c...

journal_title：Applied and environmental microbiology

authors： Olsson JC,Westerdahl A,Conway PL,Kjelleberg S

更新日期：1992-02-01 00:00:00

abstract：:The relationship between the production of reduced oxygen species, hydrogen peroxide (H(2)O(2)), superoxide (O(2)), and hydroxyl radical (.OH), and the oxidation of synthetic lignin to CO(2) was studied in whole cultures of the white-rot fungus Phanerochaete chrysosporium Burds. The kinetics of the synthesis of H(2)O(...

journal_title：Applied and environmental microbiology

authors： Faison BD,Kirk TK

更新日期：1983-11-01 00:00:00

abstract：:A nonsporulating strain of Streptomyces diastaticus producing alpha-L-arabinofuranosidase activity (EC 3.2-1.55) was isolated from soil. Two alpha-L-arabinosidases were purified by ion-exchange chromatography and chromatofocusing. The enzymes had molecular weights of 38,000 (C1) and 60,000 (C2) and pIs of 8.8 and 8.3,...

journal_title：Applied and environmental microbiology

authors： Tajana E,Fiechter A,Zimmermann W

更新日期：1992-05-01 00:00:00

abstract：:Soil microbial community characterization is increasingly being used to determine the responses of soils to stress and disturbances and to assess ecosystem sustainability. However, there is little experimental evidence to indicate that predictable patterns in microbial community structure or composition occur during s...

journal_title：Applied and environmental microbiology

authors： Banning NC,Gleeson DB,Grigg AH,Grant CD,Andersen GL,Brodie EL,Murphy DV

更新日期：2011-09-01 00:00:00

abstract：:Rsan-ver, a strain of Pseudomonas aeruginosa isolated at this department, was used for the development of a continuous process for biosurfactant production. The active compounds were identified as rhamnolipids. A final medium for production was designed in continuous culture by means of medium shifts, since the format...

journal_title：Applied and environmental microbiology

authors： Guerra-Santos L,Käppeli O,Fiechter A

更新日期：1984-08-01 00:00:00

abstract：:Yersinia spp. were isolated from 34 of 500 birds representing nine species. The highest isolation rate, 5 of 21 (23.8%), was found in blue magpies (Cyanopia cyanus), followed by pheasants (Phasianus colchicus tohkaidi), 5 of 33 (15.2%); gray starlings (Sturnus cineraceus), 6 of 57 (10.5%); tree sparrows (Passer montan...

journal_title：Applied and environmental microbiology

authors： Kato Y,Ito K,Kubokura Y,Maruyama T,Kaneko K,Ogawa M

更新日期：1985-01-01 00:00:00

abstract：:Gordonia polyisoprenivorans strain VH2, a potent rubber-degrading actinomycete, harbors two latex clearing proteins (Lcps), which are known to be essential for the microbial degradation of rubber. However, biochemical information on the exact role of this protein in the degradation of polyisoprene was lacking. In this...

journal_title：Applied and environmental microbiology

authors： Hiessl S,Böse D,Oetermann S,Eggers J,Pietruszka J,Steinbüchel A

更新日期：2014-09-01 00:00:00

abstract：:Traditional microscope-based estimates of species richness of aquatic hyphomycetes depend upon the ability of the species in the community to sporulate. Molecular techniques which detect DNA from all stages of the life cycle could potentially circumvent the problems associated with traditional methods. Leaf disks from...

journal_title：Applied and environmental microbiology

authors： Nikolcheva LG,Cockshutt AM,Bärlocher F

更新日期：2003-05-01 00:00:00

abstract：:Three cases of Mycobacterium avium complex-related lung disorders were associated with two poorly maintained spa pools by genotypic investigations. Inadequate disinfection of the two spas had reduced the load of environmental bacteria to less than 1 CFU/ml but allowed levels of M. avium complex of 4.3 x 10(4) and 4.5 ...

journal_title：Applied and environmental microbiology

authors： Lumb R,Stapledon R,Scroop A,Bond P,Cunliffe D,Goodwin A,Doyle R,Bastian I

更新日期：2004-08-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:Glutaminase is an enzyme that catalyzes the hydrolysis of l-glutamine to l-glutamate, and it plays an important role in the production of fermented foods by enhancing the umami taste. By using the genome sequence and expressed sequence tag data available for Aspergillus oryzae RIB40, we cloned a novel glutaminase gene...

journal_title：Applied and environmental microbiology

authors： Ito K,Matsushima K,Koyama Y

更新日期：2012-08-01 00:00:00

abstract：:The moderately halophilic bacterium Halobacillus halophilus carries a homologue of LuxS, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxS of H. halophilus is part of an operon that encodes an S-adenosylmethionine-dependent...

journal_title：Applied and environmental microbiology

authors： Sewald X,Saum SH,Palm P,Pfeiffer F,Oesterhelt D,Müller V

更新日期：2007-01-01 00:00:00

abstract：:Botulinum neurotoxin (BoNT) serotype B (BoNT/B) is one of the serotypes of BoNT that causes deadly human botulism, though it is used clinically for treatment of many neuromuscular diseases. BoNT/B is produced by Clostridium botulinum, and it is secreted along with a group of neurotoxin-associated proteins (NAPs) in th...

journal_title：Applied and environmental microbiology

authors： Wang HH,Riding S,Lindo P,Singh BR

更新日期：2010-10-01 00:00:00

abstract：:In a previous study by our group, CH4 oxidation and N2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsib...

journal_title：Applied and environmental microbiology

authors： Bao Z,Okubo T,Kubota K,Kasahara Y,Tsurumaru H,Anda M,Ikeda S,Minamisawa K

更新日期：2014-08-01 00:00:00

abstract：:Moistened barley was inoculated with 1.4 x 10(3) and 1.4 x 10(5) spores, respectively, from ochratoxin A-producing strains of Aspergillus ochraceus and Penicillium varidicatum. To estimate fungal tissue in the barley, the amount of glucosamine was followed for 28 days at 10 and 25 degrees C. Ochratoxin A was also foll...

journal_title：Applied and environmental microbiology

authors： Häggblom P

更新日期：1982-05-01 00:00:00

abstract：:Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid ...

journal_title：Applied and environmental microbiology

authors： Annweiler E,Materna A,Safinowski M,Kappler A,Richnow HH,Michaelis W,Meckenstock RU

更新日期：2000-12-01 00:00:00

abstract：:A method was developed to study the biodegradation and oxidative biodehalogenation of fluorinated phenols by 19F nuclear magnetic resonance (NMR). Characterization of the 19F NMR spectra of metabolite profiles of a series of fluorophenols, converted by purified phenol hydroxylase, catechol 1,2-dioxygenase, and/or by t...

journal_title：Applied and environmental microbiology

authors： Boersma MG,Dinarieva TY,Middelhoven WJ,van Berkel WJ,Doran J,Vervoort J,Rietjens IM

更新日期：1998-04-01 00:00:00

abstract：:Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...

journal_title：Applied and environmental microbiology

authors： Morick D,Faigenbaum O,Smirnov M,Fellig Y,Inbal A,Kotler M

更新日期：2015-05-15 00:00:00

abstract：:An efficient method for the transformation of the methylotrophic bacterium Methylobacterium extorquens NR-2 with a broad-host-range plasmid, pLA2917, by electroporation was examined. Transformants of M. extorquens NR-2 expressing resistance to kanamycin were obtained after electric pulse. These transformants were foun...

journal_title：Applied and environmental microbiology

authors： Ueda S,Matsumoto S,Shimizu S,Yamane T

更新日期：1991-04-01 00:00:00

abstract：:A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi has become a model ...

journal_title：Applied and environmental microbiology

authors： Kannisto M,Aho T,Karp M,Santala V

更新日期：2014-11-01 00:00:00

abstract：:The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2003-06-01 00:00:00