Transformation of a Methylotrophic Bacterium, Methylobacterium extorquens, with a Broad-Host-Range Plasmid by Electroporation.

abstract：:Bacteriophage resistance mechanisms which are derived from a bacteriophage genome are termed Per (phage-encoded resistance). When present in trans in Lactococcus lactis NCK203, Per50, the cloned origin of replication from phage phi50, interferes with phi50 replication. The per50 fragment was found to afford negligible...

journal_title：Applied and environmental microbiology

authors： O'sullivan DJ,Hill C,Klaenhammer TR

更新日期：1993-08-01 00:00:00

abstract：:The industrially important organism Corynebacterium glutamicum has been characterized in recent years for its robust ability to assimilate aromatic compounds. In this study, C. glutamicum strain AS 1.542 was investigated for its ability to catabolize phenylacetic acid (PAA). The paa genes were identified; they are org...

journal_title：Applied and environmental microbiology

authors： Chen X,Kohl TA,Rückert C,Rodionov DA,Li LH,Ding JY,Kalinowski J,Liu SJ

更新日期：2012-08-01 00:00:00

abstract：:The chlorine resistance of cysts of the flagellate protozoan Giardia muris was examined. This organism, which is pathogenic to mice, is being considered as a model for the inactivation of the human pathogen Giardia lamblia. Excystation was used as the criterion for cyst viability. Experiments were performed at pH 5, 7...

journal_title：Applied and environmental microbiology

authors： Leahy JG,Rubin AJ,Sproul OJ

更新日期：1987-07-01 00:00:00

abstract：:The occurrence and distribution of magnetotactic bacteria (MB) were studied as a function of the physical and chemical conditions in meromictic Salt Pond, Falmouth, Mass., throughout summer 2002. Three dominant MB morphotypes were observed to occur within the chemocline. Small microaerophilic magnetite-producing cocci...

journal_title：Applied and environmental microbiology

authors： Simmons SL,Sievert SM,Frankel RB,Bazylinski DA,Edwards KJ

更新日期：2004-10-01 00:00:00

abstract：:The survival of microorganisms can be predicted through the use of active biological containment systems. We have constructed contained Pseudomonas putida strains that degrade alkylbenzoates. The modified strain carries a fusion of the Plac promoter to the gef gene, which encodes a killing protein. Expression from Pla...

journal_title：Applied and environmental microbiology

authors： Ronchel MC,Ramos C,Jensen LB,Molin S,Ramos JL

更新日期：1995-08-01 00:00:00

abstract：:Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...

journal_title：Applied and environmental microbiology

authors： Morick D,Faigenbaum O,Smirnov M,Fellig Y,Inbal A,Kotler M

更新日期：2015-05-15 00:00:00

abstract：:"Viili," a fermented milk product, has a firm but viscous consistency. It is produced with traditional mesophilic mixed-strain starters, which have various stabilities in dairy practice. Thirteen morphologically different types of phages were found in 90 viili samples studied by electron microscopy. Ten of the phage t...

journal_title：Applied and environmental microbiology

authors： Saxelin ML,Nurmiaho-Lassila EL,Meriläinen VT,Forsén RI

更新日期：1986-10-01 00:00:00

abstract：:Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA (PsJN::gusA11) genes w...

journal_title：Applied and environmental microbiology

authors： Compant S,Reiter B,Sessitsch A,Nowak J,Clément C,Ait Barka E

更新日期：2005-04-01 00:00:00

abstract：:Gram-positive rubber degraders such as Streptomyces sp. strain K30 cleave rubber [poly(cis-1,4-isoprene)] to low-molecular-mass oligoisoprenoid products with terminal keto and aldehyde groups by the secretion of a latex clearing protein (Lcp) designated rubber oxygenase. LcpK30 is a heme b cytochrome and has a domain ...

journal_title：Applied and environmental microbiology

authors： Röther W,Austen S,Birke J,Jendrossek D

更新日期：2016-10-27 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:Dairy propionic acid bacteria, particularly the species Propionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lys...

journal_title：Applied and environmental microbiology

authors： Hervé C,Coste A,Rouault A,Fraslin JM,Gautier M

更新日期：2001-01-01 00:00:00

abstract：:Aureobasidium pullulans, a cosmopolitan yeast-like fungus, colonizes leaf surfaces and has potential as a biocontrol agent of pathogens. To assess the feasibility of rRNA as a target for A. pullulans-specific oligonucleotide probes, we compared the nucleotide sequences of the small-subunit rRNA (18S) genes of 12 geogr...

journal_title：Applied and environmental microbiology

authors： Li S,Cullen D,Hjort M,Spear R,Andrews JH

更新日期：1996-05-01 00:00:00

abstract：:Elevated winter losses of managed honeybee colonies are a major concern, but the underlying mechanisms remain controversial. Among the suspects are the parasitic mite Varroa destructor, the microsporidian Nosema ceranae, and associated viruses. Here we hypothesize that pathogens reduce the life expectancy of winter be...

journal_title：Applied and environmental microbiology

authors： Dainat B,Evans JD,Chen YP,Gauthier L,Neumann P

更新日期：2012-02-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, is an invasive maize pest that has spread from the Americas into Africa and Asia and causes severe crop damage worldwide. Most populations of S. frugiperda show low susceptibility to Bacillus thuringiensis (Bt) Cry1Ab or Cry1Ac toxins, which have been proved to be effective ag...

journal_title：Applied and environmental microbiology

authors： Gómez I,Ocelotl J,Sánchez J,Aguilar-Medel S,Peña-Chora G,Lina-Garcia L,Bravo A,Soberón M

更新日期：2020-10-28 00:00:00

abstract：:An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...

journal_title：Applied and environmental microbiology

authors： Russell EG

更新日期：1979-02-01 00:00:00

abstract：:Microscopic eukaryotes are known to have important ecosystem functions, but their diversity in most environments remains vastly unexplored. Here we analyzed an 18S rRNA gene library from a subsurface iron- and sulfur-oxidizing microbial community growing in highly acidic (pH < 0.9) runoff within the Richmond Mine at I...

journal_title：Applied and environmental microbiology

authors： Baker BJ,Tyson GW,Goosherst L,Banfield JF

更新日期：2009-04-01 00:00:00

abstract：:Agmatine, a significant polyamine in bacteria and plants, mostly arises from the decarboxylation of arginine. The functional importance of agmatine in fungi is poorly understood. The metabolism of agmatine and related guanidinium group-containing compounds in Aspergillus niger was explored through growth, metabolite, ...

journal_title：Applied and environmental microbiology

authors： Kumar S,Saragadam T,Punekar NS

更新日期：2015-08-15 00:00:00

abstract：:Although research has increasingly focused on the pathogenesis of avian pathogenic Escherichia coli (APEC) infections and the "APEC pathotype" itself, little is known about the reservoirs of these bacteria. We therefore compared outbreak strains isolated from diseased chickens (n = 121) with nonoutbreak strains, inclu...

journal_title：Applied and environmental microbiology

authors： Ewers C,Antão EM,Diehl I,Philipp HC,Wieler LH

更新日期：2009-01-01 00:00:00

abstract：:Plant polyphenols are of great interest for drug discovery and drug development since many of these compounds have health-promoting activities as treatments against various diseases, such as diabetes, cancer, or heart diseases. However, the limited availability of polyphenols represents a major obstacle to clinical ap...

journal_title：Applied and environmental microbiology

authors： van Summeren-Wesenhagen PV,Marienhagen J

更新日期：2015-02-01 00:00:00

abstract：:This study measured the environmental variability which exists in a commercial aerobic composting process. The specific process studied is carried out in six decomposition cells which present six different phases of the process. Temperature, pH, and moisture content were determined in several randomly chosen sample si...

journal_title：Applied and environmental microbiology

authors： Atchley SH,Clark JB

更新日期：1979-12-01 00:00:00

abstract：:A Pseudomonas putida strain (MC4) that can utilize 2,3-dichloro-1-propanol (DCP) and several aliphatic haloacids and haloalcohols as sole carbon and energy source for growth was isolated from contaminated soil. Degradation of DCP was found to start with oxidation and concomitant dehalogenation catalyzed by a 72-kDa mo...

journal_title：Applied and environmental microbiology

authors： Arif MI,Samin G,van Leeuwen JG,Oppentocht J,Janssen DB

更新日期：2012-09-01 00:00:00

abstract：:For PCR detection of Coxiella burnetii in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, hea...

journal_title：Applied and environmental microbiology

authors： Lorenz H,Jäger C,Willems H,Baljer G

更新日期：1998-11-01 00:00:00

abstract：:Rates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73 °C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the...

journal_title：Applied and environmental microbiology

authors： Urschel MR,Kubo MD,Hoehler TM,Peters JW,Boyd ES

更新日期：2015-06-01 00:00:00

abstract：:Streptomyces coelicolor and Lemna minor were used as a model to study the modulation of bacterial gene expression during plant-streptomycete interactions. S. coelicolor was grown in minimal medium with and without L. minor fronds. Bacterial proteomes were analyzed by two-dimensional gel electrophoresis, and a comparis...

journal_title：Applied and environmental microbiology

authors： Langlois P,Bourassa S,Poirier GG,Beaulieu C

更新日期：2003-04-01 00:00:00

abstract：:O-antigen biosynthetic (wbf) regions for Vibrio cholerae serogroups O5, O8, and O108 were isolated and sequenced. Sequences were compared to those of other published V. cholerae O-antigen regions. These wbf regions showed a high degree of heterogeneity both in gene content and in gene order. Genes identified frequentl...

journal_title：Applied and environmental microbiology

authors： Aydanian A,Tang L,Morris JG,Johnson JA,Stine OC

更新日期：2011-04-01 00:00:00

abstract：UNLABELLED:High-throughput sequencing (HTS) is revolutionizing environmental surveys of microbial diversity in the three domains of life by providing detailed information on which taxa are present in microbial assemblages. However, it is still unclear how the relative abundance of specific taxa gathered by HTS correlat...

journal_title：Applied and environmental microbiology

authors： Giner CR,Forn I,Romac S,Logares R,de Vargas C,Massana R

更新日期：2016-07-15 00:00:00

abstract：:The facultative anaerobe Thauera aromatica strain AR-1 uses 3,5-dihydroxybenzoate (3,5-DHB) as a sole carbon and energy source under anoxic conditions using an unusual oxidative strategy to overcome aromatic ring stability. A 25-kb gene cluster organized in four main operons encodes the anaerobic degradation pathway f...

journal_title：Applied and environmental microbiology

authors： Pacheco-Sánchez D,Molina-Fuentes Á,Marín P,Díaz-Romero A,Marqués S

更新日期：2019-01-09 00:00:00

abstract：:A major taxon of obligate marine bacteria within the order Actinomycetales has been discovered from ocean sediments. Populations of these bacteria (designated MAR 1) are persistent and widespread, spanning at least three distinct ocean systems. In this study, 212 actinomycete isolates possessing MAR 1 morphologies wer...

journal_title：Applied and environmental microbiology

authors： Mincer TJ,Jensen PR,Kauffman CA,Fenical W

更新日期：2002-10-01 00:00:00

abstract：:Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...

journal_title：Applied and environmental microbiology

authors： He R,Wooller MJ,Pohlman JW,Quensen J,Tiedje JM,Leigh MB

更新日期：2012-07-01 00:00:00

abstract：:The microbial production of biofuels and other added-value chemicals is often limited by the intrinsic toxicity of these compounds. The phasin PhaP from the soil bacterium Azotobacter sp. strain FA8 is a polyhydroxyalkanoate granule-associated protein that protects recombinant Escherichia coli against several kinds of...

journal_title：Applied and environmental microbiology

authors： Mezzina MP,Álvarez DS,Egoburo DE,Díaz Peña R,Nikel PI,Pettinari MJ

更新日期：2017-06-30 00:00:00