Metabolic engineering of Escherichia coli for the synthesis of the plant polyphenol pinosylvin.

abstract：:Escherichia coli ATCC 12806 was exposed to increasing subinhibitory concentrations of three biocides widely used in food industry facilities: trisodium phosphate (TSP), sodium nitrite (SNI), and sodium hypochlorite (SHY). The cultures exhibited an acquired tolerance to biocides (especially to SNI and SHY) after exposu...

journal_title：Applied and environmental microbiology

authors： Capita R,Riesco-Peláez F,Alonso-Hernando A,Alonso-Calleja C

更新日期：2014-02-01 00:00:00

abstract：:rRNA genetic markers were analyzed in 97 isolates of nodule bacteria from six legume species in Chihuahua, Mexico. The most common genotypes were widely shared across host species and had 16S rRNA sequences identical to those of strains from an eastern North American legume (Amphicarpaea) that are closely related to B...

journal_title：Applied and environmental microbiology

authors： Parker MA

更新日期：2002-04-01 00:00:00

abstract：:The haloalkane dehalogenase enzyme DmmA was identified by marine metagenomic screening. Determination of its crystal structure revealed an unusually large active site compared to those of previously characterized haloalkane dehalogenases. Here we present a biochemical characterization of this interesting enzyme with e...

journal_title：Applied and environmental microbiology

authors： Buryska T,Babkova P,Vavra O,Damborsky J,Prokop Z

更新日期：2018-01-02 00:00:00

abstract：:Thermophilic actinomycetes and bacilli were recovered from mushroom compost by conventional dilution plating and sedimentation chamber-Andersen sampler methods. Excessive growth of thermophilic bacilli on dilution plates accounted for the poor recovery and limited diversity of actinomycete colonies, and this result wa...

journal_title：Applied and environmental microbiology

authors： Amner W,McCarthy AJ,Edwards C

更新日期：1988-12-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and ...

journal_title：Applied and environmental microbiology

authors： Shin H,Lee JH,Yoon H,Kang DH,Ryu S

更新日期：2014-01-01 00:00:00

abstract：:Two novel gull-specific quantitative PCR (qPCR) assays were developed using 16S rRNA gene sequences from gull fecal clone libraries: a SYBR green assay targeting Streptococcus spp. (gull3) and a hydrolysis TaqMan assay targeting Catellicoccus marimammalium (gull4). The objectives of this study were to compare the host...

journal_title：Applied and environmental microbiology

authors： Ryu H,Griffith JF,Khan IU,Hill S,Edge TA,Toledo-Hernandez C,Gonzalez-Nieves J,Santo Domingo J

更新日期：2012-03-01 00:00:00

abstract：:Recent outbreaks linked to Salmonella-contaminated produce heightened the need to develop simple, rapid, and accurate detection methods, particularly those capable of determining cell viability. In this study, we examined a novel strategy for the rapid detection and quantification of viable salmonellae in produce by c...

journal_title：Applied and environmental microbiology

authors： Chen S,Wang F,Beaulieu JC,Stein RE,Ge B

更新日期：2011-06-01 00:00:00

abstract：:A new genus and species of a nonmotile gram-negative rod, Syntrophobacter wolinii, is the first bacterium described which degrades propionate only in coculture with an H(2)-using organism and in the absence of light or exogenous electron acceptors such as O(2), sulfate, or nitrate. It was isolated from methanogenic en...

journal_title：Applied and environmental microbiology

authors： Boone DR,Bryant MP

更新日期：1980-09-01 00:00:00

abstract：:Integrated air sampling and PCR-based methods for detecting airborne fungal spores, using Penicillium roqueforti as a model fungus, are described. P. roqueforti spores were collected directly into Eppendorf tubes using a miniature cyclone-type air sampler. They were then suspended in 0.1% Nonidet P-40, and counted usi...

journal_title：Applied and environmental microbiology

authors： Williams RH,Ward E,McCartney HA

更新日期：2001-06-01 00:00:00

abstract：:NisI confers immunity against nisin, with high substrate specificity to prevent a suicidal effect in nisin-producing Lactococcus lactis strains. However, the NisI maturation process as well as its influence on nisin resistance has not been characterized. Here, we report the roles of lipoprotein signal peptidase II (Ls...

journal_title：Applied and environmental microbiology

authors： Liu J,Xiong H,Du Y,Wiwatanaratanabutr I,Wu X,Zhao G,Zhu H,Caiyin Q,Qiao J

更新日期：2020-09-17 00:00:00

abstract：:Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

journal_title：Applied and environmental microbiology

authors： Bergholz TM,Vanaja SK,Whittam TS

更新日期：2009-06-01 00:00:00

abstract：:Large-scale composting facilities are known to cause environmental problems, mainly through pungent air emitted by composting material. In air samples taken above stacks set up to prepare compost used as a substrate in mushroom cultivation, several volatile compounds were identified by means of the coupled techniques ...

journal_title：Applied and environmental microbiology

authors： Derikx PJ,Op Den Camp HJ,van der Drift C,van Griensven LJ,Vogels GD

更新日期：1990-01-01 00:00:00

abstract：:Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of t...

journal_title：Applied and environmental microbiology

authors： Kästner M,Breuer-Jammali M,Mahro B

更新日期：1998-01-01 00:00:00

abstract：:The enzymatic production of 2,5-furandicarboxylic acid (FDCA) from 5-hydroxymethylfurfural (HMF) has gained interest in recent years, as FDCA is a renewable precursor of poly(ethylene-2,5-furandicarboxylate) (PEF). 5-Hydroxymethylfurfural oxidases (HMFOs) form a flavoenzyme family with genes annotated in a dozen bacte...

journal_title：Applied and environmental microbiology

authors： Viñambres M,Espada M,Martínez AT,Serrano A

更新日期：2020-08-03 00:00:00

abstract：:From Bradyrhizobium japonicum highly reiterated sequence-possessing (HRS) strains indigenous to Niigata and Tokachi in Japan with high copy numbers of the repeated sequences RSalpha and RSbeta (K. Minamisawa, T. Isawa, Y. Nakatsuka, and N. Ichikawa, Appl. Environ. Microbiol. 64:1845-1851, 1998), several insertion sequ...

journal_title：Applied and environmental microbiology

authors： Isawa T,Sameshima R,Mitsui H,Minamisawa K

更新日期：1999-08-01 00:00:00

abstract：:The synergistic antimicrobial effects of phytic acid (PA), a natural extract from rice bran, plus sodium chloride against Escherichia coli O157:H7 were examined. Exposure to NaCl alone at concentrations up to 36% (wt/wt) for 5 min did not reduce bacterial populations. The bactericidal effects of PA alone were much gre...

journal_title：Applied and environmental microbiology

authors： Kim NH,Rhee MS

更新日期：2015-12-04 00:00:00

abstract：:Rhodobacter capsulatus E1F1, a phototrophic purple nonsulfur bacterium capable of photoassimilating nitrate or nitrite, grew phototrophically in the presence of mono- and dinitrophenols with acetate as a carbon source, the highest growth levels being obtained under microaerobic conditions. Utilization of 2,4-dinitroph...

journal_title：Applied and environmental microbiology

authors： Blasco R,Castillo F

更新日期：1992-02-01 00:00:00

abstract：:In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica en...

journal_title：Applied and environmental microbiology

authors： Winkelmann N,Jaekel U,Meyer C,Serrano W,Rachel R,Rosselló-Mora R,Harder J

更新日期：2010-02-01 00:00:00

abstract：:Nitrobacter agilis was entrapped in calcium alginate beads and used as a floating bed supplied with a continuous flow of nitrite medium. Complete nitrite oxidation was achieved within 30 h, and the system could be maintained for at least 210 h. The immobilized Nitrobacter system was subjected to sulfur oxyanions, acid...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Schlasner SM,Tuovinen OH

更新日期：1986-12-01 00:00:00

abstract：:Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are...

journal_title：Applied and environmental microbiology

authors： McCabe PM,Van Alfen NK

更新日期：1999-12-01 00:00:00

abstract：:Bacillus subtilis induces expression of the gene ytnP in the presence of the antimicrobial streptomycin, produced by the Gram-positive bacterium Streptomyces griseus. ytnP encodes a lactonase-homologous protein that is able to inhibit the signaling pathway required for the streptomycin production and development of ae...

journal_title：Applied and environmental microbiology

authors： Schneider J,Yepes A,Garcia-Betancur JC,Westedt I,Mielich B,López D

更新日期：2012-01-01 00:00:00

abstract：:Primary cell cultures, a continuous cell line, and a diploid cell line were grown on an artificial capillary system. The cells were subsequently infected with Venezuelan equine encephalitis virus, and viral replication was studied. Extracellular fluids harvested from this system contained high titers of virus and were...

journal_title：Applied and environmental microbiology

authors： Johnson AD,Eddy GA,Gangemi JD,Ramsburg HH,Metzger JF

更新日期：1978-02-01 00:00:00

abstract：:The flagellate Caduceia versatilis in the gut of the termite Cryptotermes cavifrons reportedly propels itself not by its own flagella but solely by the flagella of ectosymbiotic bacteria. Previous microscopic observations have revealed that the motility symbionts are flagellated rods partially embedded in the host cel...

journal_title：Applied and environmental microbiology

authors： Hongoh Y,Sato T,Dolan MF,Noda S,Ui S,Kudo T,Ohkuma M

更新日期：2007-10-01 00:00:00

abstract：:1-Hydroxybenzotriazole, violuric acid, and N-hydroxyacetanilide are three N-OH compounds capable of mediating a range of laccase-catalyzed biotransformations, such as paper pulp delignification and degradation of polycyclic hydrocarbons. The mechanism of their enzymatic oxidation was studied with seven fungal laccases...

journal_title：Applied and environmental microbiology

authors： Xu F,Kulys JJ,Duke K,Li K,Krikstopaitis K,Deussen HJ,Abbate E,Galinyte V,Schneider P

更新日期：2000-05-01 00:00:00

abstract：:We have analyzed the impact of surface-to-volume ratio on final bacterial concentrations after batch growth. We examined six bottle sizes (20 to 1,000 ml) using three independent enumeration methods to quantify growth. We found no evidence of a so-called volumetric bottle effect, thus contradicting numerous previous r...

journal_title：Applied and environmental microbiology

authors： Hammes F,Vital M,Egli T

更新日期：2010-02-01 00:00:00

abstract：:Norovirus is the leading cause of food-related illness in the United States, and contamination of ready-to-eat items by food handlers poses a high risk for disease. This study reports the in vitro (suspension test) and in vivo (fingerpad protocol) assessments of a new ethanol-based hand sanitizer containing a synergis...

journal_title：Applied and environmental microbiology

authors： Macinga DR,Sattar SA,Jaykus LA,Arbogast JW

更新日期：2008-08-01 00:00:00

abstract：:A study of six child care centers was conducted to assess the microbiological quality of three food contact surfaces (one food serving surface and two food preparation surfaces) and one non-food contact surface (diaper changing surface) to determine the effectiveness of cleaning and sanitization procedures within the ...

journal_title：Applied and environmental microbiology

authors： Cosby CM,Costello CA,Morris WC,Haughton B,Devereaux MJ,Harte F,Davidson PM

更新日期：2008-11-01 00:00:00

abstract：:Two yellow pigments were isolated for the first time from the entomopathogenic bacterium Photorhabdus luminescens in liquid culture and were identified as the anthraquinone derivatives 3,8-dimethoxy-1-hydroxy-9,10-anthraquinone (minor) and 1,3-dimethoxy-8-hydroxy-9,10-anthraquinone (major). A known antibiotic, 3,5-dih...

journal_title：Applied and environmental microbiology

authors： Li J,Chen G,Wu H,Webster JM

更新日期：1995-12-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00