The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway.

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:Pathogens may reach agricultural soils through application of animal manure and thereby pose a risk of contaminating crops as well as surface and groundwater. Treatment and handling of manure for improved nutrient and odor management may also influence the amount and fate of manure-borne pathogens in the soil. A study...

journal_title：Applied and environmental microbiology

authors： Amin MG,Forslund A,Bui XT,Juhler RK,Petersen SO,Lægdsmand M

更新日期：2013-01-01 00:00:00

abstract：:Bacteria in the genus Rickettsiella (Coxiellaceae), which are mainly known as arthropod pathogens, are emerging as excellent models to study transitions between mutualism and pathogenicity. The current report characterizes a novel Rickettsiella found in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae), a m...

journal_title：Applied and environmental microbiology

authors： Iasur-Kruh L,Weintraub PG,Mozes-Daube N,Robinson WE,Perlman SJ,Zchori-Fein E

更新日期：2013-07-01 00:00:00

abstract：:The sensitivity of different microorganisms, considered as typical representatives of the microflora of soil and water, was established to evaluate the influence of the nonmedical use of antimicrobial agents on bacterial ecology. Only seven strains, six chemoorganotrophs and one chemolithotroph, could be considered as...

journal_title：Applied and environmental microbiology

authors： Van Dijck P,van de Voorde H

更新日期：1976-03-01 00:00:00

abstract：:Here, we report a novel method to produce microencapsulated enzymes using Saccharomyces cerevisiae spores. In sporulating cells, soluble secreted proteins are transported to the spore wall. Previous work has shown that the spore wall is capable of retaining soluble proteins because its outer layers work as a diffusion...

journal_title：Applied and environmental microbiology

authors： Shi L,Li Z,Tachikawa H,Gao XD,Nakanishi H

更新日期：2014-08-01 00:00:00

abstract：:The aerobic intestinal microflora of 2-week-old herring (Clupea harengus) larvae was characterized by using conventional microbiological methods and electron microscopy. Larvae were hatched and kept in filtered seawater or in seawater with penicillin and streptomycin. The gastrointestinal tract of herring larvae is es...

journal_title：Applied and environmental microbiology

authors： Hansen GH,Strøm E,Olafsen JA

更新日期：1992-02-01 00:00:00

abstract：:We report the characterization of the ccpA gene of Lactobacillus plantarum, coding for catabolite control protein A. The gene is linked to the pepQ gene, encoding a proline peptidase, in the order ccpA-pepQ, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. Two ccpA trans...

journal_title：Applied and environmental microbiology

authors： Muscariello L,Marasco R,De Felice M,Sacco M

更新日期：2001-07-01 00:00:00

abstract：:Estimations of the bacterial content of air can be more easily made now than a decade ago, with colony formation the method of choice for enumeration of airborne bacteria. However, plate counts are subject to error because bacteria exposed to the air may remain viable yet lose the ability to form colonies, i.e., they ...

journal_title：Applied and environmental microbiology

authors： Heidelberg JF,Shahamat M,Levin M,Rahman I,Stelma G,Grim C,Colwell RR

更新日期：1997-09-01 00:00:00

abstract：:The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...

journal_title：Applied and environmental microbiology

authors： Dinoto A,Marques TM,Sakamoto K,Fukiya S,Watanabe J,Ito S,Yokota A

更新日期：2006-12-01 00:00:00

abstract：:Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of ...

journal_title：Applied and environmental microbiology

authors： Dunlap PV,Davis KM,Tomiyama S,Fujino M,Fukui A

更新日期：2008-12-01 00:00:00

abstract：:Denitrification is an alternative type of anaerobic respiration in which nitrate is reduced to gaseous products via nitrite. The key step in this process is the reduction of nitrite to nitric oxide, which is catalyzed by two structurally different but functionally equivalent forms of nitrite reductase encoded by the n...

journal_title：Applied and environmental microbiology

authors： Pratscher J,Stichternoth C,Fichtl K,Schleifer KH,Braker G

更新日期：2009-02-01 00:00:00

abstract：:The ammonia-oxidizing prokaryote (AOP) community in three groundwater treatment plants and connected distribution systems was analyzed by quantitative real-time PCR and sequence analysis targeting the amoA gene of ammonia-oxidizing bacteria (AOB) and archaea (AOA). Results demonstrated that AOB and AOA numbers increas...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Voost S,van der Kooij D

更新日期：2009-07-01 00:00:00

abstract：:We examined physiological adaptations which allow the psychrotroph Rhodococcus sp. strain Q15 to assimilate alkanes at a low temperature (alkanes are contaminants which are generally insoluble and/or solid at low temperatures). During growth at 5 degrees C on hexadecane or diesel fuel, strain Q15 produced a cell surfa...

journal_title：Applied and environmental microbiology

authors： Whyte LG,Slagman SJ,Pietrantonio F,Bourbonnière L,Koval SF,Lawrence JR,Inniss WE,Greer CW

更新日期：1999-07-01 00:00:00

abstract：:The alphaproteobacterium Magnetospirillum gryphiswaldense has the intriguing ability to navigate within magnetic fields, a behavior named magnetotaxis, governed by the formation of magnetosomes, intracellular membrane-enveloped crystals of magnetite. Magnetosomes are aligned in chains along the cell's motility axis by...

journal_title：Applied and environmental microbiology

authors： Pfeiffer D,Schüler D

更新日期：2020-01-21 00:00:00

abstract：:A gene that codes for a novel intracellular poly(3-hydroxybutyrate) (PHB) depolymerase, designated PhaZ1, has been identified in the genome of Bacillus megaterium. A native PHB (nPHB) granule-binding assay showed that purified soluble PhaZ1 had strong affinity for nPHB granules. Turbidimetric analyses revealed that Ph...

journal_title：Applied and environmental microbiology

authors： Chen HJ,Pan SC,Shaw GC

更新日期：2009-08-01 00:00:00

abstract：:Pyrobaculum islandicum uses iron, thiosulfate, and elemental sulfur for anaerobic respiration, while Pyrobaculum aerophilum uses iron and nitrate; however, the constraints on these processes and their physiological mechanisms for iron and sulfur reduction are not well understood. Growth rates on sulfur compounds are h...

journal_title：Applied and environmental microbiology

authors： Feinberg LF,Srikanth R,Vachet RW,Holden JF

更新日期：2008-01-01 00:00:00

abstract：:ω-Transaminase (ω-TA) is a promising enzyme for use in the production of unnatural amino acids from keto acids using cheap amino donors such as isopropylamine. The small substrate-binding pocket of most ω-TAs permits entry of substituents no larger than an ethyl group, which presents a significant challenge to the pre...

journal_title：Applied and environmental microbiology

authors： Han SW,Park ES,Dong JY,Shin JS

更新日期：2015-10-01 00:00:00

abstract：:Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit. Isolates that had low levels of resistance (hereafter referred to as LR isolates) and high levels of resist...

journal_title：Applied and environmental microbiology

authors： Ma Z,Yoshimura MA,Michailides TJ

更新日期：2003-12-01 00:00:00

abstract：UNLABELLED:Salmonella is recognized as one of the most significant enteric foodborne bacterial pathogens. In recent years, the resistance of pathogens to biocides and other environmental stresses, especially when they are embedded in biofilm structures, has led to the search for and development of novel antimicrobial s...

journal_title：Applied and environmental microbiology

authors： Karampoula F,Giaouris E,Deschamps J,Doulgeraki AI,Nychas GJ,Dubois-Brissonnet F

更新日期：2016-08-15 00:00:00

abstract：:The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid o...

journal_title：Applied and environmental microbiology

authors： Koyama Y,Okamoto S,Furukawa K

更新日期：1990-07-01 00:00:00

abstract：:Chlorhexidine (CHX) is a broad-spectrum antiseptic widely used in community and clinical contexts for many years that has recently acquired higher relevance in nosocomial infection control worldwide. Despite this, CHX tolerance among Enterococcus faecium bacteria, representing one of the leading agents causing nosocom...

journal_title：Applied and environmental microbiology

authors： Duarte B,Pereira AP,Freitas AR,Coque TM,Hammerum AM,Hasman H,Antunes P,Peixe L,Novais C

更新日期：2019-11-14 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00

abstract：:Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electri...

journal_title：Applied and environmental microbiology

authors： Zhang W,Hughes J,Chen Y

更新日期：2012-06-01 00:00:00

abstract：:A database was probed with artificial neural network (ANN) and multivariate logistic regression (MLR) models to investigate the efficacy of predicting PCR-identified human adenovirus (ADV), Norwalk-like virus (NLV), and enterovirus (EV) presence or absence in shellfish harvested from diverse countries in Europe (Spain...

journal_title：Applied and environmental microbiology

authors： Brion G,Viswanathan C,Neelakantan TR,Lingireddy S,Girones R,Lees D,Allard A,Vantarakis A

更新日期：2005-09-01 00:00:00

abstract：:The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...

journal_title：Applied and environmental microbiology

authors： Garneau MÈ,Schnetzer A,Countway PD,Jones AC,Seubert EL,Caron DA

更新日期：2011-11-01 00:00:00

abstract：:This study examined the inactivation of human norovirus (HuNoV) GI.1 and GII.4 by chlorine under conditions mimicking sewage treatment. Using a porcine gastric mucin-magnetic bead (PGM-MB) assay, no statistically significant loss in HuNoV binding (inactivation) was observed for secondary effluent treatments of ≤25 ppm...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Fay JP,Calci K,Pouillot R,Woods J,Chen H,Niemira BA,Van Doren JM

更新日期：2017-11-16 00:00:00

abstract：:The gut microbiota plays important roles in its host. However, how each microbiota member contributes to the behavior of the whole population is not known. In this study, we therefore determined protein expression in the cecal microbiota in chickens of selected ages and in 7-day-old chickens inoculated with different ...

journal_title：Applied and environmental microbiology

authors： Polansky O,Sekelova Z,Faldynova M,Sebkova A,Sisak F,Rychlik I

更新日期：2015-12-28 00:00:00

abstract：:Sodium salicylate and ammonium sulfate were applied to leaf surfaces along with suspensions of the biological control agents Pseudomonas syringae Cit7(pNAH7), which catabolizes salicylate, and Cit7, which does not catabolize salicylate, to determine whether enhanced biological control of bacterial speck of tomato coul...

journal_title：Applied and environmental microbiology

authors： Ji P,Wilson M

更新日期：2003-02-01 00:00:00

abstract：:Although it is widely believed that horizontal patchiness exists in microbial sediment communities, determining the extent of variability or the particular members of the bacterial community which account for the observed differences among sites at various scales has not been routinely demonstrated. In this study, hor...

journal_title：Applied and environmental microbiology

authors： Scala DJ,Kerkhof LJ

更新日期：2000-05-01 00:00:00

abstract：:Indirect pathogenicity (IP), the commensal protection of antibiotic-sensitive pathogens by resistant microorganisms of low intrinsic virulence, can prevent the eradication of polymicrobial infections. The contributions of antibiotic resistance mechanisms and biofilm structure to IP within polymicrobial biofilms were i...

journal_title：Applied and environmental microbiology

authors： O'Connell HA,Kottkamp GS,Eppelbaum JL,Stubblefield BA,Gilbert SE,Gilbert ES

更新日期：2006-07-01 00:00:00